Cover Image

Optimization of milk-based medium for efficient cultivation of Bifidobacerium pseudocatenulatum G4 using face-centered central composite-response surface methodology

This study was undertaken to optimize skim milk and yeast extract concentration as a cultivation medium for optimal Bifidobacteria pseudocatenulatum G4 (G4) biomass and β-galactosidase production as well as lactose and free amino nitrogen (FAN) balance after cultivation period. Optimization process...

Full description

Saved in:
Bibliographic Details
Main Authors: Abdul Khalil, Khalilah, Mustafa, Shuhaimi, Mohammad, Rosfarizan, Ariff, Arbakariya, Shaari, Yamin, Abdul Manap, Mohd. Yazid, Ahmad, Siti Aqlima, Dahalan, Farrah Aini
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2014
Online Access:http://psasir.upm.edu.my/id/eprint/34776/1/Optimization%20of%20Milk-Based%20Medium%20for%20Efficient%20Cultivation.pdf
http://psasir.upm.edu.my/id/eprint/34776/
http://www.hindawi.com/journals/bmri/2014/787989/abs/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.34776
record_format eprints
spelling my.upm.eprints.347762015-12-23T01:04:47Z http://psasir.upm.edu.my/id/eprint/34776/ Optimization of milk-based medium for efficient cultivation of Bifidobacerium pseudocatenulatum G4 using face-centered central composite-response surface methodology Abdul Khalil, Khalilah Mustafa, Shuhaimi Mohammad, Rosfarizan Ariff, Arbakariya Shaari, Yamin Abdul Manap, Mohd. Yazid Ahmad, Siti Aqlima Dahalan, Farrah Aini This study was undertaken to optimize skim milk and yeast extract concentration as a cultivation medium for optimal Bifidobacteria pseudocatenulatum G4 (G4) biomass and β-galactosidase production as well as lactose and free amino nitrogen (FAN) balance after cultivation period. Optimization process in this study involved four steps: screening for significant factors using 23 full factorial design, steepest ascent, optimization using FCCD-RSM, and verification. From screening steps, skim milk and yeast extract showed significant influence on the biomass production and, based on the steepest ascent step, middle points of skim milk (6% wt/vol) and yeast extract (1.89% wt/vol) were obtained. A polynomial regression model in FCCD-RSM revealed that both factors were found significant and the strongest influence was given by skim milk concentration. Optimum concentrations of skim milk and yeast extract for maximum biomass G4 and β-galactosidase production meanwhile low in lactose and FAN balance after cultivation period were 5.89% (wt/vol) and 2.31% (wt/vol), respectively. The validation experiments showed that the predicted and experimental values are not significantly different, indicating that the FCCD-RSM model developed is sufficient to describe the cultivation process of G4 using skim-milk-based medium with the addition of yeast extract. Hindawi Publishing Corporation 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34776/1/Optimization%20of%20Milk-Based%20Medium%20for%20Efficient%20Cultivation.pdf Abdul Khalil, Khalilah and Mustafa, Shuhaimi and Mohammad, Rosfarizan and Ariff, Arbakariya and Shaari, Yamin and Abdul Manap, Mohd. Yazid and Ahmad, Siti Aqlima and Dahalan, Farrah Aini (2014) Optimization of milk-based medium for efficient cultivation of Bifidobacerium pseudocatenulatum G4 using face-centered central composite-response surface methodology. BioMed Research International, 2014. art. no. 787989. pp. 1-10. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2014/787989/abs/ 10.1155/2014/787989
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This study was undertaken to optimize skim milk and yeast extract concentration as a cultivation medium for optimal Bifidobacteria pseudocatenulatum G4 (G4) biomass and β-galactosidase production as well as lactose and free amino nitrogen (FAN) balance after cultivation period. Optimization process in this study involved four steps: screening for significant factors using 23 full factorial design, steepest ascent, optimization using FCCD-RSM, and verification. From screening steps, skim milk and yeast extract showed significant influence on the biomass production and, based on the steepest ascent step, middle points of skim milk (6% wt/vol) and yeast extract (1.89% wt/vol) were obtained. A polynomial regression model in FCCD-RSM revealed that both factors were found significant and the strongest influence was given by skim milk concentration. Optimum concentrations of skim milk and yeast extract for maximum biomass G4 and β-galactosidase production meanwhile low in lactose and FAN balance after cultivation period were 5.89% (wt/vol) and 2.31% (wt/vol), respectively. The validation experiments showed that the predicted and experimental values are not significantly different, indicating that the FCCD-RSM model developed is sufficient to describe the cultivation process of G4 using skim-milk-based medium with the addition of yeast extract.
format Article
author Abdul Khalil, Khalilah
Mustafa, Shuhaimi
Mohammad, Rosfarizan
Ariff, Arbakariya
Shaari, Yamin
Abdul Manap, Mohd. Yazid
Ahmad, Siti Aqlima
Dahalan, Farrah Aini
spellingShingle Abdul Khalil, Khalilah
Mustafa, Shuhaimi
Mohammad, Rosfarizan
Ariff, Arbakariya
Shaari, Yamin
Abdul Manap, Mohd. Yazid
Ahmad, Siti Aqlima
Dahalan, Farrah Aini
Optimization of milk-based medium for efficient cultivation of Bifidobacerium pseudocatenulatum G4 using face-centered central composite-response surface methodology
author_facet Abdul Khalil, Khalilah
Mustafa, Shuhaimi
Mohammad, Rosfarizan
Ariff, Arbakariya
Shaari, Yamin
Abdul Manap, Mohd. Yazid
Ahmad, Siti Aqlima
Dahalan, Farrah Aini
author_sort Abdul Khalil, Khalilah
title Optimization of milk-based medium for efficient cultivation of Bifidobacerium pseudocatenulatum G4 using face-centered central composite-response surface methodology
title_short Optimization of milk-based medium for efficient cultivation of Bifidobacerium pseudocatenulatum G4 using face-centered central composite-response surface methodology
title_full Optimization of milk-based medium for efficient cultivation of Bifidobacerium pseudocatenulatum G4 using face-centered central composite-response surface methodology
title_fullStr Optimization of milk-based medium for efficient cultivation of Bifidobacerium pseudocatenulatum G4 using face-centered central composite-response surface methodology
title_full_unstemmed Optimization of milk-based medium for efficient cultivation of Bifidobacerium pseudocatenulatum G4 using face-centered central composite-response surface methodology
title_sort optimization of milk-based medium for efficient cultivation of bifidobacerium pseudocatenulatum g4 using face-centered central composite-response surface methodology
publisher Hindawi Publishing Corporation
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/34776/1/Optimization%20of%20Milk-Based%20Medium%20for%20Efficient%20Cultivation.pdf
http://psasir.upm.edu.my/id/eprint/34776/
http://www.hindawi.com/journals/bmri/2014/787989/abs/
_version_ 1643831255681204224
score 13.232389

Similar Items