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Morpho-molecular analysis as a prognostic model for repulsive feedback of the medicinal plant "Andrographis paniculata" to allogamy

Andrographis paniculata Nees. (AP) is a self-pollinated medicinal herb with a wide range of pharmaceutical properties, facing a low diversity in Malaysia. Cross-pollination of AP accessions leads to considerable rates of heterosis in the agro-morphological characteristics and anticancer phytochemica...

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Main Authors: Valdiani, Alireza, Talei, Daryush, Javanmard, Arash, Tan, Soon Guan, Abdul Kadir, Mihdzar, Mahmood, Maziah
Format: Article
Published: Elsevier 2014
Online Access:http://psasir.upm.edu.my/id/eprint/34605/
http://www.sciencedirect.com/science/article/pii/S0378111914003503
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spelling my.upm.eprints.346052015-12-16T03:36:00Z http://psasir.upm.edu.my/id/eprint/34605/ Morpho-molecular analysis as a prognostic model for repulsive feedback of the medicinal plant "Andrographis paniculata" to allogamy Valdiani, Alireza Talei, Daryush Javanmard, Arash Tan, Soon Guan Abdul Kadir, Mihdzar Mahmood, Maziah Andrographis paniculata Nees. (AP) is a self-pollinated medicinal herb with a wide range of pharmaceutical properties, facing a low diversity in Malaysia. Cross-pollination of AP accessions leads to considerable rates of heterosis in the agro-morphological characteristics and anticancer phytochemicals of this eminent medicinal herb. However, the poor crossability of the plant at the interpopulation or intraspecific levels is an obstacle from the evolutionary and breeding points of view as an average of 4.56% crossability was recorded for AP in this study. Hence, this research aimed to elicit the impact of parental genetic distances (GDs) on the rate of crossability of AP using seven accessions in 21 possible cross combinations. To this end, a set of 55 randomly amplified polymorphic DNA (RAPD) primers and a total of 13 agro-morphological markers were employed to test the hypothesis. Twenty-two out of the 55 RAPD primers amplified a total of 257 bands of which 107 bands were found to be polymorphic. The principal component analysis (PCA) based on the RAPD markers revealed that the studied AP accessions were distributed to three distinct groups. Furthermore, it was noticed that even a minor increase in GD between two parents can cause a decline in their crossability. Unlike, the morphological-based GDs acted neutrally to crossability. This finding suggests that, despite the low genetic diversity among the Malaysian APs, a population prescreening using RAPD markers would be useful to enhance the rate of fruit set through selecting the genetically adjacent parents. Elsevier 2014-06-01 Article PeerReviewed Valdiani, Alireza and Talei, Daryush and Javanmard, Arash and Tan, Soon Guan and Abdul Kadir, Mihdzar and Mahmood, Maziah (2014) Morpho-molecular analysis as a prognostic model for repulsive feedback of the medicinal plant "Andrographis paniculata" to allogamy. Gene, 542 (2). pp. 156-167. ISSN 0378-1119; ESSN: 1879-0038 http://www.sciencedirect.com/science/article/pii/S0378111914003503 10.1016/j.gene.2014.03.039
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description Andrographis paniculata Nees. (AP) is a self-pollinated medicinal herb with a wide range of pharmaceutical properties, facing a low diversity in Malaysia. Cross-pollination of AP accessions leads to considerable rates of heterosis in the agro-morphological characteristics and anticancer phytochemicals of this eminent medicinal herb. However, the poor crossability of the plant at the interpopulation or intraspecific levels is an obstacle from the evolutionary and breeding points of view as an average of 4.56% crossability was recorded for AP in this study. Hence, this research aimed to elicit the impact of parental genetic distances (GDs) on the rate of crossability of AP using seven accessions in 21 possible cross combinations. To this end, a set of 55 randomly amplified polymorphic DNA (RAPD) primers and a total of 13 agro-morphological markers were employed to test the hypothesis. Twenty-two out of the 55 RAPD primers amplified a total of 257 bands of which 107 bands were found to be polymorphic. The principal component analysis (PCA) based on the RAPD markers revealed that the studied AP accessions were distributed to three distinct groups. Furthermore, it was noticed that even a minor increase in GD between two parents can cause a decline in their crossability. Unlike, the morphological-based GDs acted neutrally to crossability. This finding suggests that, despite the low genetic diversity among the Malaysian APs, a population prescreening using RAPD markers would be useful to enhance the rate of fruit set through selecting the genetically adjacent parents.
format Article
author Valdiani, Alireza
Talei, Daryush
Javanmard, Arash
Tan, Soon Guan
Abdul Kadir, Mihdzar
Mahmood, Maziah
spellingShingle Valdiani, Alireza
Talei, Daryush
Javanmard, Arash
Tan, Soon Guan
Abdul Kadir, Mihdzar
Mahmood, Maziah
Morpho-molecular analysis as a prognostic model for repulsive feedback of the medicinal plant "Andrographis paniculata" to allogamy
author_facet Valdiani, Alireza
Talei, Daryush
Javanmard, Arash
Tan, Soon Guan
Abdul Kadir, Mihdzar
Mahmood, Maziah
author_sort Valdiani, Alireza
title Morpho-molecular analysis as a prognostic model for repulsive feedback of the medicinal plant "Andrographis paniculata" to allogamy
title_short Morpho-molecular analysis as a prognostic model for repulsive feedback of the medicinal plant "Andrographis paniculata" to allogamy
title_full Morpho-molecular analysis as a prognostic model for repulsive feedback of the medicinal plant "Andrographis paniculata" to allogamy
title_fullStr Morpho-molecular analysis as a prognostic model for repulsive feedback of the medicinal plant "Andrographis paniculata" to allogamy
title_full_unstemmed Morpho-molecular analysis as a prognostic model for repulsive feedback of the medicinal plant "Andrographis paniculata" to allogamy
title_sort morpho-molecular analysis as a prognostic model for repulsive feedback of the medicinal plant "andrographis paniculata" to allogamy
publisher Elsevier
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/34605/
http://www.sciencedirect.com/science/article/pii/S0378111914003503
_version_ 1643831204245405696
score 13.211869

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