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A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain)

An efficient shoot bud differentiation and multiple shoot induction from leaf derived callus of Lablab bean (Lablab purpureus var. lignosus) have been obtained. Callus induction and shoot multiplication at various frequencies were observed using different concentrations and combinations of auxins (I...

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Main Authors: Thiruvengadam, Muthu, Jayabalan, Narayanasamypillai
Format: Article
Language:English
Published: Universiti Putra Malaysia Press 2003
Online Access:http://psasir.upm.edu.my/id/eprint/3460/1/Protocol_for_Efficient_Plantlet_Regeneration_from_Leaf_Derived_Callus.pdf
http://psasir.upm.edu.my/id/eprint/3460/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2026%20(1)%20Apr.%202003/06%20JTAS%20Vol.26%20(1)%202003%20(Pg%2035-40).pdf
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spelling my.upm.eprints.34602015-09-11T02:54:56Z http://psasir.upm.edu.my/id/eprint/3460/ A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain) Thiruvengadam, Muthu Jayabalan, Narayanasamypillai An efficient shoot bud differentiation and multiple shoot induction from leaf derived callus of Lablab bean (Lablab purpureus var. lignosus) have been obtained. Callus induction and shoot multiplication at various frequencies were observed using different concentrations and combinations of auxins (IAA, 2, 4-D and NAA) and cytokinin (RAP). The highest frequency of callus induction was observed on MS medium containing 2, 4-D (3.0 mg/1) and RAP (0.5 mg/1). The green compact nodular calli occurred on NAA (3.0 mg/1) and BAP (0.5 mg/1). Highest percentage of shoot bud formation and multiplication was obtained from a combination of RAP (2.0 mg/1) and NAA (0.5 mg/1). The regenerated shoots were transferred to MS medium containing IBA (1.5 mg/1) for the induction of roots. Rooted plants were transferred to plastic cups and subsequently these were successfully transferred to fields. Universiti Putra Malaysia Press 2003 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/3460/1/Protocol_for_Efficient_Plantlet_Regeneration_from_Leaf_Derived_Callus.pdf Thiruvengadam, Muthu and Jayabalan, Narayanasamypillai (2003) A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain). Pertanika Journal of Tropical Agricultural Science, 26 (1). pp. 35-40. ISSN 1511-3701 http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2026%20(1)%20Apr.%202003/06%20JTAS%20Vol.26%20(1)%202003%20(Pg%2035-40).pdf
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description An efficient shoot bud differentiation and multiple shoot induction from leaf derived callus of Lablab bean (Lablab purpureus var. lignosus) have been obtained. Callus induction and shoot multiplication at various frequencies were observed using different concentrations and combinations of auxins (IAA, 2, 4-D and NAA) and cytokinin (RAP). The highest frequency of callus induction was observed on MS medium containing 2, 4-D (3.0 mg/1) and RAP (0.5 mg/1). The green compact nodular calli occurred on NAA (3.0 mg/1) and BAP (0.5 mg/1). Highest percentage of shoot bud formation and multiplication was obtained from a combination of RAP (2.0 mg/1) and NAA (0.5 mg/1). The regenerated shoots were transferred to MS medium containing IBA (1.5 mg/1) for the induction of roots. Rooted plants were transferred to plastic cups and subsequently these were successfully transferred to fields.
format Article
author Thiruvengadam, Muthu
Jayabalan, Narayanasamypillai
spellingShingle Thiruvengadam, Muthu
Jayabalan, Narayanasamypillai
A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain)
author_facet Thiruvengadam, Muthu
Jayabalan, Narayanasamypillai
author_sort Thiruvengadam, Muthu
title A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain)
title_short A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain)
title_full A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain)
title_fullStr A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain)
title_full_unstemmed A protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (Lablab purpureus var. lignosus (L) prain)
title_sort protocol for efficient plantlet regeneration from leaf derived callus of lablab bean (lablab purpureus var. lignosus (l) prain)
publisher Universiti Putra Malaysia Press
publishDate 2003
url http://psasir.upm.edu.my/id/eprint/3460/1/Protocol_for_Efficient_Plantlet_Regeneration_from_Leaf_Derived_Callus.pdf
http://psasir.upm.edu.my/id/eprint/3460/
http://www.pertanika.upm.edu.my/Pertanika%20PAPERS/JTAS%20Vol.%2026%20(1)%20Apr.%202003/06%20JTAS%20Vol.26%20(1)%202003%20(Pg%2035-40).pdf
_version_ 1643822617492193280
score 13.211869

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