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Molecular cloning of a putative Acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (AeNCED) and its overexpression in rice

9-Cis-epoxycarotenoid dioxygenase (NCED) is an important enzyme which catalyzes the oxidative cleavage of abscisic acid (ABA), a plant growth regulator which plays a vital role in stress tolerance. In this study, a cDNA sequence encoding NCED from a mangrove plant (Acanthus ebracteatus) was analyzed...

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Main Authors: Sultana, Shahanaz, Turečková, Veronika, Ho, Chai Ling, Napis, Suhaimi, Namasivayam, Parameswari
Format: Article
Language:English
Published: Korean Society of Crop Science 2014
Online Access:http://psasir.upm.edu.my/id/eprint/34576/1/Molecular%20cloning%20of%20a%20putative%20Acanthus%20ebracteatus.pdf
http://psasir.upm.edu.my/id/eprint/34576/
http://link.springer.com/article/10.1007%2Fs12892-014-0006-4
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spelling my.upm.eprints.345762016-01-22T03:12:14Z http://psasir.upm.edu.my/id/eprint/34576/ Molecular cloning of a putative Acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (AeNCED) and its overexpression in rice Sultana, Shahanaz Turečková, Veronika Ho, Chai Ling Napis, Suhaimi Namasivayam, Parameswari 9-Cis-epoxycarotenoid dioxygenase (NCED) is an important enzyme which catalyzes the oxidative cleavage of abscisic acid (ABA), a plant growth regulator which plays a vital role in stress tolerance. In this study, a cDNA sequence encoding NCED from a mangrove plant (Acanthus ebracteatus) was analyzed and overexpressed in rice. Analysis of the deduced amino acid sequence of AeNCED revealed an open reading frame of 1,638 bp encoding a protein of 545 amino acids, with a 123 bp 5’-untranslated region (UTR) and a 259 bp 3’-UTR. The deduced amino acid sequence of AeNCED is more than 80% identical to the amino acid sequences of carotenoid cleavage dioxygenase from carrot, tomato, and coffee. The RNA encoding AeNCED was detected in transgenic rice (Oryza sativa cv. BRRI dhan29) plants overexpressing this cDNA. These plants only showed significantly higher tolerance to salinity at germination and better performance at seedling stages. The levels of ABA in transgenic rice seedlings overexpressing AeNCED treated with 100 mM NaCl for 24 hours were higher than those of untransformed plants. However, a higher level of dihydrophaseic acid (DPA) and ABA glucose ester (ABA-GE) were also observed in these transgenic plants suggesting that rapid degradation of ABA through a self-regulation mechanism. Korean Society of Crop Science 2014-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34576/1/Molecular%20cloning%20of%20a%20putative%20Acanthus%20ebracteatus.pdf Sultana, Shahanaz and Turečková, Veronika and Ho, Chai Ling and Napis, Suhaimi and Namasivayam, Parameswari (2014) Molecular cloning of a putative Acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (AeNCED) and its overexpression in rice. Journal of Crop Science and Biotechnology, 17 (4). pp. 239-246. ISSN 1975-9479; ESSN: 2005-8276 http://link.springer.com/article/10.1007%2Fs12892-014-0006-4 10.1007/s12892-014-0006-4
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description 9-Cis-epoxycarotenoid dioxygenase (NCED) is an important enzyme which catalyzes the oxidative cleavage of abscisic acid (ABA), a plant growth regulator which plays a vital role in stress tolerance. In this study, a cDNA sequence encoding NCED from a mangrove plant (Acanthus ebracteatus) was analyzed and overexpressed in rice. Analysis of the deduced amino acid sequence of AeNCED revealed an open reading frame of 1,638 bp encoding a protein of 545 amino acids, with a 123 bp 5’-untranslated region (UTR) and a 259 bp 3’-UTR. The deduced amino acid sequence of AeNCED is more than 80% identical to the amino acid sequences of carotenoid cleavage dioxygenase from carrot, tomato, and coffee. The RNA encoding AeNCED was detected in transgenic rice (Oryza sativa cv. BRRI dhan29) plants overexpressing this cDNA. These plants only showed significantly higher tolerance to salinity at germination and better performance at seedling stages. The levels of ABA in transgenic rice seedlings overexpressing AeNCED treated with 100 mM NaCl for 24 hours were higher than those of untransformed plants. However, a higher level of dihydrophaseic acid (DPA) and ABA glucose ester (ABA-GE) were also observed in these transgenic plants suggesting that rapid degradation of ABA through a self-regulation mechanism.
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author Sultana, Shahanaz
Turečková, Veronika
Ho, Chai Ling
Napis, Suhaimi
Namasivayam, Parameswari
spellingShingle Sultana, Shahanaz
Turečková, Veronika
Ho, Chai Ling
Napis, Suhaimi
Namasivayam, Parameswari
Molecular cloning of a putative Acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (AeNCED) and its overexpression in rice
author_facet Sultana, Shahanaz
Turečková, Veronika
Ho, Chai Ling
Napis, Suhaimi
Namasivayam, Parameswari
author_sort Sultana, Shahanaz
title Molecular cloning of a putative Acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (AeNCED) and its overexpression in rice
title_short Molecular cloning of a putative Acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (AeNCED) and its overexpression in rice
title_full Molecular cloning of a putative Acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (AeNCED) and its overexpression in rice
title_fullStr Molecular cloning of a putative Acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (AeNCED) and its overexpression in rice
title_full_unstemmed Molecular cloning of a putative Acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (AeNCED) and its overexpression in rice
title_sort molecular cloning of a putative acanthus ebracteatus- 9-cis-epoxycarotenoid deoxygenase (aenced) and its overexpression in rice
publisher Korean Society of Crop Science
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/34576/1/Molecular%20cloning%20of%20a%20putative%20Acanthus%20ebracteatus.pdf
http://psasir.upm.edu.my/id/eprint/34576/
http://link.springer.com/article/10.1007%2Fs12892-014-0006-4
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score 13.211869

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