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Membrane-active antibacterial compounds in methanolic extracts of Jatropha curcas and their mode of action against Staphylococcus aureus S1434 and Escherichia coli E216

This research presents the antibacterial potential and mode of action of related active compounds of kernel meal, leaves, stem bark, root bark and root wood extracts of Jatropha curcas Linn. plant on Staphylococcus aureus S1434 and Escherichia coli E216. At double MIC (minimum inhibitory concentrati...

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Main Authors: Aidah, Namuli, Abdullah, Norhani, Oskoueian, Ehsan, Sieo, Chin Chin, Saad, Wan Zuhainis
Format: Article
Language:English
Published: Friends Science Publishers 2014
Online Access:http://psasir.upm.edu.my/id/eprint/34489/1/Membrane-active%20antibacterial%20compounds%20in%20methanolic%20extracts%20of%20Jatropha%20curcas%20and%20their%20mode%20of%20action%20against%20Staphylococcus%20aureus%20S1434%20and%20Escherichia%20coli%20E216.pdf
http://psasir.upm.edu.my/id/eprint/34489/
http://www.fspublishers.org/Issue.php?categoryID=125
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spelling my.upm.eprints.344892016-09-19T02:21:46Z http://psasir.upm.edu.my/id/eprint/34489/ Membrane-active antibacterial compounds in methanolic extracts of Jatropha curcas and their mode of action against Staphylococcus aureus S1434 and Escherichia coli E216 Aidah, Namuli Abdullah, Norhani Oskoueian, Ehsan Sieo, Chin Chin Saad, Wan Zuhainis This research presents the antibacterial potential and mode of action of related active compounds of kernel meal, leaves, stem bark, root bark and root wood extracts of Jatropha curcas Linn. plant on Staphylococcus aureus S1434 and Escherichia coli E216. At double MIC (minimum inhibitory concentration) value, cell viability of S. aureus S1434 was inhibited by all extracts, but only kernel meal and root wood extracts inhibited E. coli E216. At half MIC, the μ24 (decrease in cell viability after 24h) for S. aureus S1434 was 69 and 66%, while that of E. coli E216 were 44 and 42% in the presence of kernel meal and leaves extract, respectively. However at double MIC, less than 5% of viable cells of S. aureus S1434 were detected in the leaves and root bark extracts after 5h. Conversely, less than 5% of the viable cells of E. coli E216 were detected in the presence of kernel meal and root wood extract after 7.5h. Loss of 260nm absorbing compounds and proteins from bacterial cells was directly proportional to the time of exposure of cells to the extracts. All extracts caused bacterial cells to lose their ability to tolerate salt (NaCl) at double MIC value. The loss of 260nm absorbing compounds, proteins and the loss of tolerance to NaCl suggest that leaves, root bark and kernel meal damaged the bacterial cell membrane. The analysis of bioactive compounds by GC-MS confirmed the presence of acetic acid, hexadecanoic acid, citric acid, 9-octadecenoic acid as the major membrane-active antibacterial compounds. Friends Science Publishers 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34489/1/Membrane-active%20antibacterial%20compounds%20in%20methanolic%20extracts%20of%20Jatropha%20curcas%20and%20their%20mode%20of%20action%20against%20Staphylococcus%20aureus%20S1434%20and%20Escherichia%20coli%20E216.pdf Aidah, Namuli and Abdullah, Norhani and Oskoueian, Ehsan and Sieo, Chin Chin and Saad, Wan Zuhainis (2014) Membrane-active antibacterial compounds in methanolic extracts of Jatropha curcas and their mode of action against Staphylococcus aureus S1434 and Escherichia coli E216. International Journal of Agriculture and Biology, 16 (4). pp. 723-730. ISSN 1560-8530; ESSN: 1814-9596 http://www.fspublishers.org/Issue.php?categoryID=125
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description This research presents the antibacterial potential and mode of action of related active compounds of kernel meal, leaves, stem bark, root bark and root wood extracts of Jatropha curcas Linn. plant on Staphylococcus aureus S1434 and Escherichia coli E216. At double MIC (minimum inhibitory concentration) value, cell viability of S. aureus S1434 was inhibited by all extracts, but only kernel meal and root wood extracts inhibited E. coli E216. At half MIC, the μ24 (decrease in cell viability after 24h) for S. aureus S1434 was 69 and 66%, while that of E. coli E216 were 44 and 42% in the presence of kernel meal and leaves extract, respectively. However at double MIC, less than 5% of viable cells of S. aureus S1434 were detected in the leaves and root bark extracts after 5h. Conversely, less than 5% of the viable cells of E. coli E216 were detected in the presence of kernel meal and root wood extract after 7.5h. Loss of 260nm absorbing compounds and proteins from bacterial cells was directly proportional to the time of exposure of cells to the extracts. All extracts caused bacterial cells to lose their ability to tolerate salt (NaCl) at double MIC value. The loss of 260nm absorbing compounds, proteins and the loss of tolerance to NaCl suggest that leaves, root bark and kernel meal damaged the bacterial cell membrane. The analysis of bioactive compounds by GC-MS confirmed the presence of acetic acid, hexadecanoic acid, citric acid, 9-octadecenoic acid as the major membrane-active antibacterial compounds.
format Article
author Aidah, Namuli
Abdullah, Norhani
Oskoueian, Ehsan
Sieo, Chin Chin
Saad, Wan Zuhainis
spellingShingle Aidah, Namuli
Abdullah, Norhani
Oskoueian, Ehsan
Sieo, Chin Chin
Saad, Wan Zuhainis
Membrane-active antibacterial compounds in methanolic extracts of Jatropha curcas and their mode of action against Staphylococcus aureus S1434 and Escherichia coli E216
author_facet Aidah, Namuli
Abdullah, Norhani
Oskoueian, Ehsan
Sieo, Chin Chin
Saad, Wan Zuhainis
author_sort Aidah, Namuli
title Membrane-active antibacterial compounds in methanolic extracts of Jatropha curcas and their mode of action against Staphylococcus aureus S1434 and Escherichia coli E216
title_short Membrane-active antibacterial compounds in methanolic extracts of Jatropha curcas and their mode of action against Staphylococcus aureus S1434 and Escherichia coli E216
title_full Membrane-active antibacterial compounds in methanolic extracts of Jatropha curcas and their mode of action against Staphylococcus aureus S1434 and Escherichia coli E216
title_fullStr Membrane-active antibacterial compounds in methanolic extracts of Jatropha curcas and their mode of action against Staphylococcus aureus S1434 and Escherichia coli E216
title_full_unstemmed Membrane-active antibacterial compounds in methanolic extracts of Jatropha curcas and their mode of action against Staphylococcus aureus S1434 and Escherichia coli E216
title_sort membrane-active antibacterial compounds in methanolic extracts of jatropha curcas and their mode of action against staphylococcus aureus s1434 and escherichia coli e216
publisher Friends Science Publishers
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/34489/1/Membrane-active%20antibacterial%20compounds%20in%20methanolic%20extracts%20of%20Jatropha%20curcas%20and%20their%20mode%20of%20action%20against%20Staphylococcus%20aureus%20S1434%20and%20Escherichia%20coli%20E216.pdf
http://psasir.upm.edu.my/id/eprint/34489/
http://www.fspublishers.org/Issue.php?categoryID=125
_version_ 1643831169286930432
score 13.211869

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