Cover Image

Mechanism(s) of action involved in the gastroprotective activity of Muntingia calabura

Ethnopharmacological relevance: Muntingia calabura L. (Muntingiaceae) is locally known as kerukup siam. Its leaves, flowers, barks and roots have been used traditionally in East Asia and South America to treat various diseases including ulcer-related diseases. The present study aimed to investigate...

Full description

Saved in:
Bibliographic Details
Main Authors: Zakaria, Zainul Amiruddin, Balan, Tavamani, Suppaiah, Velan, Ahmad, Syahida, Jamaludin, Fadzureena
Format: Article
Language:English
Published: Elsevier 2014
Online Access:http://psasir.upm.edu.my/id/eprint/34478/1/Mechanism%28s%29%20of%20action%20involved%20in%20the%20gastroprotective%20activity%20of%20Muntingia%20calabura.pdf
http://psasir.upm.edu.my/id/eprint/34478/
http://www.sciencedirect.com/science/article/pii/S0378874113009276
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.34478
record_format eprints
spelling my.upm.eprints.344782016-04-25T07:25:32Z http://psasir.upm.edu.my/id/eprint/34478/ Mechanism(s) of action involved in the gastroprotective activity of Muntingia calabura Zakaria, Zainul Amiruddin Balan, Tavamani Suppaiah, Velan Ahmad, Syahida Jamaludin, Fadzureena Ethnopharmacological relevance: Muntingia calabura L. (Muntingiaceae) is locally known as kerukup siam. Its leaves, flowers, barks and roots have been used traditionally in East Asia and South America to treat various diseases including ulcer-related diseases. The present study aimed to investigate the mechanism(s) of gastroprotective effect of methanol extract of Muntingia calabura leaves (MEMC) using the pylorus ligation induced gastric ulceration in rats. Materials and methods: Five groups of rats (n=6) were administered orally once daily for 7 days with 8% Tween 80 (negative control), 100 mg/kg ranitidine (positive control), or MEMC (100, 250 or 500 mg/kg), followed by the ulcer induction via ligation of the pyloric part of the rat’s stomach. This was followed by the macroscopic analysis of the stomach, evaluation of gastric content parameters, and quantification of mucus content. The antioxidant (measured using the superoxide anion and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, oxygen radical absorbance capacity (ORAC) and total phenolic content (TPC) assays), anti-inflammatory (evaluated using the in vitro lipoxygenase and xanthine oxidase assays), phytoconstituents and HPLC analysis of MEMC were also carried out. Results: The MEMC significantly (p<0.05) reduced gastric lesion in this model. Furthermore, the extract also significantly (p<0.01) reduced the volume of gastric content whereas the total acidity was significantly (p<0.05) reduced in the doses of 100 and 500 mg/kg MEMC. Moreover, the mucus content increased significantly (p<0.01) in MEMC-treated rats. The extract also showed high antioxidant and anti-inflammatory activities in all assays tested, and demonstrated the presence of high tannins and saponins followed by flavonoids. Conclusion: The MEMC exerted gastroprotective effect via several mechanisms including the anti-secretory, antioxidant and anti-inflammatory activities. These activities could be attributed to the presence of tannins, saponins and flavonoids (e.g. rutin, quercitrin, fisetin and dihydroquercetin). Elsevier 2014-02-12 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34478/1/Mechanism%28s%29%20of%20action%20involved%20in%20the%20gastroprotective%20activity%20of%20Muntingia%20calabura.pdf Zakaria, Zainul Amiruddin and Balan, Tavamani and Suppaiah, Velan and Ahmad, Syahida and Jamaludin, Fadzureena (2014) Mechanism(s) of action involved in the gastroprotective activity of Muntingia calabura. Journal of Ethnopharmacology, 151 (3). pp. 1184-1193. ISSN 0378-8741; ESSN: 1872-7573 http://www.sciencedirect.com/science/article/pii/S0378874113009276 10.1016/j.jep.2013.12.045
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Ethnopharmacological relevance: Muntingia calabura L. (Muntingiaceae) is locally known as kerukup siam. Its leaves, flowers, barks and roots have been used traditionally in East Asia and South America to treat various diseases including ulcer-related diseases. The present study aimed to investigate the mechanism(s) of gastroprotective effect of methanol extract of Muntingia calabura leaves (MEMC) using the pylorus ligation induced gastric ulceration in rats. Materials and methods: Five groups of rats (n=6) were administered orally once daily for 7 days with 8% Tween 80 (negative control), 100 mg/kg ranitidine (positive control), or MEMC (100, 250 or 500 mg/kg), followed by the ulcer induction via ligation of the pyloric part of the rat’s stomach. This was followed by the macroscopic analysis of the stomach, evaluation of gastric content parameters, and quantification of mucus content. The antioxidant (measured using the superoxide anion and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging, oxygen radical absorbance capacity (ORAC) and total phenolic content (TPC) assays), anti-inflammatory (evaluated using the in vitro lipoxygenase and xanthine oxidase assays), phytoconstituents and HPLC analysis of MEMC were also carried out. Results: The MEMC significantly (p<0.05) reduced gastric lesion in this model. Furthermore, the extract also significantly (p<0.01) reduced the volume of gastric content whereas the total acidity was significantly (p<0.05) reduced in the doses of 100 and 500 mg/kg MEMC. Moreover, the mucus content increased significantly (p<0.01) in MEMC-treated rats. The extract also showed high antioxidant and anti-inflammatory activities in all assays tested, and demonstrated the presence of high tannins and saponins followed by flavonoids. Conclusion: The MEMC exerted gastroprotective effect via several mechanisms including the anti-secretory, antioxidant and anti-inflammatory activities. These activities could be attributed to the presence of tannins, saponins and flavonoids (e.g. rutin, quercitrin, fisetin and dihydroquercetin).
format Article
author Zakaria, Zainul Amiruddin
Balan, Tavamani
Suppaiah, Velan
Ahmad, Syahida
Jamaludin, Fadzureena
spellingShingle Zakaria, Zainul Amiruddin
Balan, Tavamani
Suppaiah, Velan
Ahmad, Syahida
Jamaludin, Fadzureena
Mechanism(s) of action involved in the gastroprotective activity of Muntingia calabura
author_facet Zakaria, Zainul Amiruddin
Balan, Tavamani
Suppaiah, Velan
Ahmad, Syahida
Jamaludin, Fadzureena
author_sort Zakaria, Zainul Amiruddin
title Mechanism(s) of action involved in the gastroprotective activity of Muntingia calabura
title_short Mechanism(s) of action involved in the gastroprotective activity of Muntingia calabura
title_full Mechanism(s) of action involved in the gastroprotective activity of Muntingia calabura
title_fullStr Mechanism(s) of action involved in the gastroprotective activity of Muntingia calabura
title_full_unstemmed Mechanism(s) of action involved in the gastroprotective activity of Muntingia calabura
title_sort mechanism(s) of action involved in the gastroprotective activity of muntingia calabura
publisher Elsevier
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/34478/1/Mechanism%28s%29%20of%20action%20involved%20in%20the%20gastroprotective%20activity%20of%20Muntingia%20calabura.pdf
http://psasir.upm.edu.my/id/eprint/34478/
http://www.sciencedirect.com/science/article/pii/S0378874113009276
_version_ 1643831166038441984
score 13.211869

Similar Items