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Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers

Developing cultivars resistant against different biotypes of brown planthopper (BPH) through marker-assisted selection (MAS) is an effective management strategy to control this pest. In this study, 28 polymorphic simple sequence repeat (SSR) markers were analyzed in 108 F3 progenies derived from the...

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Main Authors: Shabanimofrad, Mahmoodreza, Yusop, Mohd Rafii, Ashkani, Sadegh, Musa, Mohamed Hanafi, Adam, Nur Azura, Iffah Haifa,, Harun, Abdul Rahim, Abdul Latif, Mohammad
Format: Article
Language:English
Published: Scientific and Technical Research Council of Turkey 2014
Online Access:http://psasir.upm.edu.my/id/eprint/34443/1/Marker-assisted%20selection%20for%20rice%20brown%20planthopper%20%28Nilaparvata%20lugens%29resistance%20using%20linked%20SSR%20markers.pdf
http://psasir.upm.edu.my/id/eprint/34443/
http://journals.tubitak.gov.tr/biology/abstract.htm?id=16911
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spelling my.upm.eprints.344432016-01-27T02:12:24Z http://psasir.upm.edu.my/id/eprint/34443/ Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers Shabanimofrad, Mahmoodreza Yusop, Mohd Rafii Ashkani, Sadegh Musa, Mohamed Hanafi Adam, Nur Azura Iffah Haifa, Harun, Abdul Rahim Abdul Latif, Mohammad Developing cultivars resistant against different biotypes of brown planthopper (BPH) through marker-assisted selection (MAS) is an effective management strategy to control this pest. In this study, 28 polymorphic simple sequence repeat (SSR) markers were analyzed in 108 F3 progenies derived from the cross of Rathu Heenati and MR276 rice cultivars to investigate the association with BPH resistance against biotypes 2 and 3. For statistical analysis, parents and their offspring were grouped into 2 phenotypic classes based on their levels of BPH resistance. Chi-square analysis demonstrated a good fit to a ratio of 3:1 for the segregation of resistance and susceptibility. Association of SSR markers with phenotypic traits in F3 progenies were identified. Six SSR markers (RM401, RM5953, RM217, RM210, RM242, and RM1103) were significantly associated with BPH resistance to biotypes 2 and 3 of BPH. Out of these 6 markers, RM401, RM5953, and RM217 accounted for about 17% of total phenotypic variation and RM210, RM242, and RM1103 accounted for 20% of total phenotypic variation against biotypes 2 and 3, respectively. Therefore, the above 6 markers were confirmed for MAS in rice breeding programs to develop BPH-resistant rice cultivars. Scientific and Technical Research Council of Turkey 2014 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34443/1/Marker-assisted%20selection%20for%20rice%20brown%20planthopper%20%28Nilaparvata%20lugens%29resistance%20using%20linked%20SSR%20markers.pdf Shabanimofrad, Mahmoodreza and Yusop, Mohd Rafii and Ashkani, Sadegh and Musa, Mohamed Hanafi and Adam, Nur Azura and Iffah Haifa, and Harun, Abdul Rahim and Abdul Latif, Mohammad (2014) Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers. Turkish Journal of Biology, 39 (5). pp. 666-673. ISSN 1300-0152; ESSN: 1303-6092 http://journals.tubitak.gov.tr/biology/abstract.htm?id=16911 10.3906/biy-1406-78
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Developing cultivars resistant against different biotypes of brown planthopper (BPH) through marker-assisted selection (MAS) is an effective management strategy to control this pest. In this study, 28 polymorphic simple sequence repeat (SSR) markers were analyzed in 108 F3 progenies derived from the cross of Rathu Heenati and MR276 rice cultivars to investigate the association with BPH resistance against biotypes 2 and 3. For statistical analysis, parents and their offspring were grouped into 2 phenotypic classes based on their levels of BPH resistance. Chi-square analysis demonstrated a good fit to a ratio of 3:1 for the segregation of resistance and susceptibility. Association of SSR markers with phenotypic traits in F3 progenies were identified. Six SSR markers (RM401, RM5953, RM217, RM210, RM242, and RM1103) were significantly associated with BPH resistance to biotypes 2 and 3 of BPH. Out of these 6 markers, RM401, RM5953, and RM217 accounted for about 17% of total phenotypic variation and RM210, RM242, and RM1103 accounted for 20% of total phenotypic variation against biotypes 2 and 3, respectively. Therefore, the above 6 markers were confirmed for MAS in rice breeding programs to develop BPH-resistant rice cultivars.
format Article
author Shabanimofrad, Mahmoodreza
Yusop, Mohd Rafii
Ashkani, Sadegh
Musa, Mohamed Hanafi
Adam, Nur Azura
Iffah Haifa,
Harun, Abdul Rahim
Abdul Latif, Mohammad
spellingShingle Shabanimofrad, Mahmoodreza
Yusop, Mohd Rafii
Ashkani, Sadegh
Musa, Mohamed Hanafi
Adam, Nur Azura
Iffah Haifa,
Harun, Abdul Rahim
Abdul Latif, Mohammad
Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers
author_facet Shabanimofrad, Mahmoodreza
Yusop, Mohd Rafii
Ashkani, Sadegh
Musa, Mohamed Hanafi
Adam, Nur Azura
Iffah Haifa,
Harun, Abdul Rahim
Abdul Latif, Mohammad
author_sort Shabanimofrad, Mahmoodreza
title Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers
title_short Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers
title_full Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers
title_fullStr Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers
title_full_unstemmed Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers
title_sort marker-assisted selection for rice brown planthopper (nilaparvata lugens)resistance using linked ssr markers
publisher Scientific and Technical Research Council of Turkey
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/34443/1/Marker-assisted%20selection%20for%20rice%20brown%20planthopper%20%28Nilaparvata%20lugens%29resistance%20using%20linked%20SSR%20markers.pdf
http://psasir.upm.edu.my/id/eprint/34443/
http://journals.tubitak.gov.tr/biology/abstract.htm?id=16911
_version_ 1643831155715211264
score 13.211869

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