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Isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight

A total of 325 bacteria were isolated from both healthy and sheath blight infected leaf samples of rice plants, collected from different places of Malaysia, following dilution technique. Sheath blight pathogen was isolated from infected samples by tissue plating method. Out of 325, 14 isolates were...

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Main Authors: Akter, Shamima, Kadir, Jugah, Juraimi, Abdul Shukor, Mohd Saud, Halimi, Elmahdi, Salha
Format: Article
Published: Triveni Enterprises 2014
Online Access:http://psasir.upm.edu.my/id/eprint/34289/
http://www.jeb.co.in/index.php?page=abstract&issue=201411_nov14&number=13
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spelling my.upm.eprints.342892015-12-10T03:21:48Z http://psasir.upm.edu.my/id/eprint/34289/ Isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight Akter, Shamima Kadir, Jugah Juraimi, Abdul Shukor Mohd Saud, Halimi Elmahdi, Salha A total of 325 bacteria were isolated from both healthy and sheath blight infected leaf samples of rice plants, collected from different places of Malaysia, following dilution technique. Sheath blight pathogen was isolated from infected samples by tissue plating method. Out of 325, 14 isolates were found to be antagonist against the pathogen in pre evaluation test. All the 14 isolates were morphologically characterized. Antagonistic activity of these isolates was further confirmed by adopting the standard dual culture and extracellular metabolite tests. The best isolates were selected, based on the results. In dual culture test, the selected bacterial isolates KMB25, TMB33, PMB38, UMB20 and BMB42 showed 68.44%, 60.89%, 60.22%, 50.00% and 48.22% fungal growth inhibition, respectively and in extracellular metabolite test these bacterial isolates exhibited 93.33%, 84.26%, 69.82%, 67.96% and 39.26% of the same, respectively. Biochemical tests of selected isolates were performed following standard procedure. These bacterial isolates were tentatively identified as fluorescent pseudomonas by morphological and biochemical characterization. The identities were further confirmed by Biolog microstation system as P. fluorescens (UMB20), P. aeruginosa (KMB25, TMB33 and PMB38) and P. asplenii (BMB42) with similarity index ranging from 0.517 to 0.697. The effective bacterial isolates obtained from the present study can be used in the management of soil borne fungal pathogen Rhizoctonia solani, causing sheath blight of rice. Triveni Enterprises 2014-11 Article NonPeerReviewed Akter, Shamima and Kadir, Jugah and Juraimi, Abdul Shukor and Mohd Saud, Halimi and Elmahdi, Salha (2014) Isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight. Journal of Environmental Biology, 35 (6). pp. 1095-1100. ISSN 0254-8704; ESSN: 2394-0379 http://www.jeb.co.in/index.php?page=abstract&issue=201411_nov14&number=13
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description A total of 325 bacteria were isolated from both healthy and sheath blight infected leaf samples of rice plants, collected from different places of Malaysia, following dilution technique. Sheath blight pathogen was isolated from infected samples by tissue plating method. Out of 325, 14 isolates were found to be antagonist against the pathogen in pre evaluation test. All the 14 isolates were morphologically characterized. Antagonistic activity of these isolates was further confirmed by adopting the standard dual culture and extracellular metabolite tests. The best isolates were selected, based on the results. In dual culture test, the selected bacterial isolates KMB25, TMB33, PMB38, UMB20 and BMB42 showed 68.44%, 60.89%, 60.22%, 50.00% and 48.22% fungal growth inhibition, respectively and in extracellular metabolite test these bacterial isolates exhibited 93.33%, 84.26%, 69.82%, 67.96% and 39.26% of the same, respectively. Biochemical tests of selected isolates were performed following standard procedure. These bacterial isolates were tentatively identified as fluorescent pseudomonas by morphological and biochemical characterization. The identities were further confirmed by Biolog microstation system as P. fluorescens (UMB20), P. aeruginosa (KMB25, TMB33 and PMB38) and P. asplenii (BMB42) with similarity index ranging from 0.517 to 0.697. The effective bacterial isolates obtained from the present study can be used in the management of soil borne fungal pathogen Rhizoctonia solani, causing sheath blight of rice.
format Article
author Akter, Shamima
Kadir, Jugah
Juraimi, Abdul Shukor
Mohd Saud, Halimi
Elmahdi, Salha
spellingShingle Akter, Shamima
Kadir, Jugah
Juraimi, Abdul Shukor
Mohd Saud, Halimi
Elmahdi, Salha
Isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight
author_facet Akter, Shamima
Kadir, Jugah
Juraimi, Abdul Shukor
Mohd Saud, Halimi
Elmahdi, Salha
author_sort Akter, Shamima
title Isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight
title_short Isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight
title_full Isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight
title_fullStr Isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight
title_full_unstemmed Isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight
title_sort isolation and identification of antagonistic bacteria from phylloplane of rice as biocontrol agents for sheath blight
publisher Triveni Enterprises
publishDate 2014
url http://psasir.upm.edu.my/id/eprint/34289/
http://www.jeb.co.in/index.php?page=abstract&issue=201411_nov14&number=13
_version_ 1643831109156339712
score 13.211869

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