Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa
Background Breast cancer is one of the most dreading types of cancer among women. Herbal medicine has becoming a potential source of treatment for breast cancer. Herbal plant Dillenia suffruticosa (Griff) Martelli under the family Dilleniaceae has been traditionally used to treat cancerous growth....
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2014
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my.upm.eprints.341672015-12-07T08:04:46Z http://psasir.upm.edu.my/id/eprint/34167/ Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa Tor, Yin Sim Saiful Yazan, Latifah Foo, Jhi Biau Nurdin, Armania Cheah, Yoke Kqueen Abdullah, Rasedee Imam, Mustapha Umar Ismail, Norsharina Ismail, Maznah Background Breast cancer is one of the most dreading types of cancer among women. Herbal medicine has becoming a potential source of treatment for breast cancer. Herbal plant Dillenia suffruticosa (Griff) Martelli under the family Dilleniaceae has been traditionally used to treat cancerous growth. In this study, the anticancer effect of ethyl acetate extract of D. suffruticosa (EADs) was examined on human breast adenocarcinoma cell line MCF-7 and the molecular pathway involved was elucidated. Methods EADs was obtained from the root of D. suffruticosa by using sequential solvent extraction. Cytotoxicity was determined by using MTT assay, mode of cell death by cell cycle analysis and apoptosis induction by Annexin-FITC/PI assay. Morphology changes in cells were observed under inverted light microscope. Involvement of selected genes in the oxidative stress-mediated signaling pathway was explored using multiplex gene expression analysis. Results The treatment of EADs caused cytotoxicity to MCF-7 cells in a dose- and time-dependent manner at 24, 48 and 72 hours with IC50 of 76 ± 2.3, 58 ± 0.7 and 39 ± 3.6 μg/mL, respectively. The IC50 of tamoxifen-treated MCF-7 cells was 8 ± 0.5 μg/mL. Induction of apoptosis by EADs was dose- and time- dependent. EADs induced non-phase specific cell cycle arrest at different concentration and time point. The multiplex mRNA expression study indicated that EADs-induced apoptosis was accompanied by upregulation of the expression of SOD1, SOD2, NF-κB, p53, p38 MAPK, and catalase, but downregulation of Akt1. Conclusion It is suggested that EADs induced apoptosis in MCF-7 cells by modulating numerous genes which are involved in oxidative stress pathway. Therefore, EADs has the potential to act as an effective intervention against breast cancer cells. BioMed Central 2014 Article PeerReviewed Tor, Yin Sim and Saiful Yazan, Latifah and Foo, Jhi Biau and Nurdin, Armania and Cheah, Yoke Kqueen and Abdullah, Rasedee and Imam, Mustapha Umar and Ismail, Norsharina and Ismail, Maznah (2014) Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa. BMC Complementary and Alternative Medicine, 14. art. no. 55. pp. 1-12. ISSN 1472-6882 http://www.biomedcentral.com/1472-6882/14/55/abstract 10.1186/1472-6882-14-55 |
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Background
Breast cancer is one of the most dreading types of cancer among women. Herbal medicine has becoming a potential source of treatment for breast cancer. Herbal plant Dillenia suffruticosa (Griff) Martelli under the family Dilleniaceae has been traditionally used to treat cancerous growth. In this study, the anticancer effect of ethyl acetate extract of D. suffruticosa (EADs) was examined on human breast adenocarcinoma cell line MCF-7 and the molecular pathway involved was elucidated.
Methods
EADs was obtained from the root of D. suffruticosa by using sequential solvent extraction. Cytotoxicity was determined by using MTT assay, mode of cell death by cell cycle analysis and apoptosis induction by Annexin-FITC/PI assay. Morphology changes in cells were observed under inverted light microscope. Involvement of selected genes in the oxidative stress-mediated signaling pathway was explored using multiplex gene expression analysis.
Results
The treatment of EADs caused cytotoxicity to MCF-7 cells in a dose- and time-dependent manner at 24, 48 and 72 hours with IC50 of 76 ± 2.3, 58 ± 0.7 and 39 ± 3.6 μg/mL, respectively. The IC50 of tamoxifen-treated MCF-7 cells was 8 ± 0.5 μg/mL. Induction of apoptosis by EADs was dose- and time- dependent. EADs induced non-phase specific cell cycle arrest at different concentration and time point. The multiplex mRNA expression study indicated that EADs-induced apoptosis was accompanied by upregulation of the expression of SOD1, SOD2, NF-κB, p53, p38 MAPK, and catalase, but downregulation of Akt1.
Conclusion
It is suggested that EADs induced apoptosis in MCF-7 cells by modulating numerous genes which are involved in oxidative stress pathway. Therefore, EADs has the potential to act as an effective intervention against breast cancer cells. |
| format |
Article |
| author |
Tor, Yin Sim Saiful Yazan, Latifah Foo, Jhi Biau Nurdin, Armania Cheah, Yoke Kqueen Abdullah, Rasedee Imam, Mustapha Umar Ismail, Norsharina Ismail, Maznah |
| spellingShingle |
Tor, Yin Sim Saiful Yazan, Latifah Foo, Jhi Biau Nurdin, Armania Cheah, Yoke Kqueen Abdullah, Rasedee Imam, Mustapha Umar Ismail, Norsharina Ismail, Maznah Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa |
| author_facet |
Tor, Yin Sim Saiful Yazan, Latifah Foo, Jhi Biau Nurdin, Armania Cheah, Yoke Kqueen Abdullah, Rasedee Imam, Mustapha Umar Ismail, Norsharina Ismail, Maznah |
| author_sort |
Tor, Yin Sim |
| title |
Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa |
| title_short |
Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa |
| title_full |
Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa |
| title_fullStr |
Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa |
| title_full_unstemmed |
Induction of apoptosis through oxidative stress-related pathways in MCF-7, human breast cancer cells, by ethyl acetate extract of Dillenia suffruticosa |
| title_sort |
induction of apoptosis through oxidative stress-related pathways in mcf-7, human breast cancer cells, by ethyl acetate extract of dillenia suffruticosa |
| publisher |
BioMed Central |
| publishDate |
2014 |
| url |
http://psasir.upm.edu.my/id/eprint/34167/ http://www.biomedcentral.com/1472-6882/14/55/abstract |
| _version_ |
1643831071976980480 |
| score |
13.244414 |