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N-Acetyl-d-glucosamine kinase and germ-tube formation in Candida albicans

N-Acetyl-d-glucosamine kinase (GlcNAc kinase, EC 2.7.1.59), the specific enzyme required for the phosphorylation ofN-acetyl-d-glucosamine (GlcNAc) toN-acetylglucosamine 6-phosphate, was found to be an inducible enzyme inCandida albicans. The pattern of GlcNAc kinase induction was examined under cond...

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Main Authors: Shepherd, Maxwell G., M. Ghazali, Hasanah, Sullivan, Patrick A.
Format: Article
Language:English
Published: Academic Press 1980
Online Access:http://psasir.upm.edu.my/id/eprint/34122/1/27.%2034122%20N-acetyl.pdf
http://psasir.upm.edu.my/id/eprint/34122/
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spelling my.upm.eprints.341222015-04-24T00:37:20Z http://psasir.upm.edu.my/id/eprint/34122/ N-Acetyl-d-glucosamine kinase and germ-tube formation in Candida albicans Shepherd, Maxwell G. M. Ghazali, Hasanah Sullivan, Patrick A. N-Acetyl-d-glucosamine kinase (GlcNAc kinase, EC 2.7.1.59), the specific enzyme required for the phosphorylation ofN-acetyl-d-glucosamine (GlcNAc) toN-acetylglucosamine 6-phosphate, was found to be an inducible enzyme inCandida albicans. The pattern of GlcNAc kinase induction was examined under conditions where germ-tube formation was elicited and where blastospores metabolized GlcNAc with no change in morphology. There was no change in either the time course or the extent of GlcNAc kinase induction, indicating that this enzyme is not a control point for the dimorphic development inC. albicans. A low basal level of GlcNAc kinase activity was found in noninduced cells ofC. albicans but this increased 20- to 30-fold after the addition of GlcNAc to the cell suspension. GlcNAc is both an inducer and a substrate for the enzyme; ATP and Mg2+ were also required for activity. TheKmvalues for GlcNAc and ATP are 1.9 and 1.3 mm, respectively. ADP was found to be a noncompetitive inhibitor with respect to GlcNAc with aKivalue of 7.1 mm. UDP-GlcNAc and glucosamine 6-phosphate had no effect on GlcNAc kinase activity. The presence of ethidium bromide (an inhibitor of transcription) or trichodermin (an inhibitor of translation) in cell suspensions ofC. albicans inhibited the increase in GlcNAc kinase specific activity. The glucose analogs, α-methylglucopyranoside and 3-O-methylglucose, enhanced the formation of germ tubes byC. albicans but the induction of GlcNAc kinase was not affected. 2-Deoxyglucose, an inhibitor of germ-tube formation, had no effect on the induction of GlcNAc kinase Academic Press 1980-06 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/34122/1/27.%2034122%20N-acetyl.pdf Shepherd, Maxwell G. and M. Ghazali, Hasanah and Sullivan, Patrick A. (1980) N-Acetyl-d-glucosamine kinase and germ-tube formation in Candida albicans. Experimental Mycology, 4 (2). pp. 147-159. ISSN 0147-5975 10.1016/0147-5975(80)90018-3
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description N-Acetyl-d-glucosamine kinase (GlcNAc kinase, EC 2.7.1.59), the specific enzyme required for the phosphorylation ofN-acetyl-d-glucosamine (GlcNAc) toN-acetylglucosamine 6-phosphate, was found to be an inducible enzyme inCandida albicans. The pattern of GlcNAc kinase induction was examined under conditions where germ-tube formation was elicited and where blastospores metabolized GlcNAc with no change in morphology. There was no change in either the time course or the extent of GlcNAc kinase induction, indicating that this enzyme is not a control point for the dimorphic development inC. albicans. A low basal level of GlcNAc kinase activity was found in noninduced cells ofC. albicans but this increased 20- to 30-fold after the addition of GlcNAc to the cell suspension. GlcNAc is both an inducer and a substrate for the enzyme; ATP and Mg2+ were also required for activity. TheKmvalues for GlcNAc and ATP are 1.9 and 1.3 mm, respectively. ADP was found to be a noncompetitive inhibitor with respect to GlcNAc with aKivalue of 7.1 mm. UDP-GlcNAc and glucosamine 6-phosphate had no effect on GlcNAc kinase activity. The presence of ethidium bromide (an inhibitor of transcription) or trichodermin (an inhibitor of translation) in cell suspensions ofC. albicans inhibited the increase in GlcNAc kinase specific activity. The glucose analogs, α-methylglucopyranoside and 3-O-methylglucose, enhanced the formation of germ tubes byC. albicans but the induction of GlcNAc kinase was not affected. 2-Deoxyglucose, an inhibitor of germ-tube formation, had no effect on the induction of GlcNAc kinase
format Article
author Shepherd, Maxwell G.
M. Ghazali, Hasanah
Sullivan, Patrick A.
spellingShingle Shepherd, Maxwell G.
M. Ghazali, Hasanah
Sullivan, Patrick A.
N-Acetyl-d-glucosamine kinase and germ-tube formation in Candida albicans
author_facet Shepherd, Maxwell G.
M. Ghazali, Hasanah
Sullivan, Patrick A.
author_sort Shepherd, Maxwell G.
title N-Acetyl-d-glucosamine kinase and germ-tube formation in Candida albicans
title_short N-Acetyl-d-glucosamine kinase and germ-tube formation in Candida albicans
title_full N-Acetyl-d-glucosamine kinase and germ-tube formation in Candida albicans
title_fullStr N-Acetyl-d-glucosamine kinase and germ-tube formation in Candida albicans
title_full_unstemmed N-Acetyl-d-glucosamine kinase and germ-tube formation in Candida albicans
title_sort n-acetyl-d-glucosamine kinase and germ-tube formation in candida albicans
publisher Academic Press
publishDate 1980
url http://psasir.upm.edu.my/id/eprint/34122/1/27.%2034122%20N-acetyl.pdf
http://psasir.upm.edu.my/id/eprint/34122/
_version_ 1643831059895287808
score 13.211869

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