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Mutation breeding of microbial strain (Streptomyces fradiae) for increased antibiotic yield

A strain improvement programme for higher yield has been attempted for a local anti- biotic-producing strain, Streptomyces fradiae. An empirical approach of sequential mutation-selection and rediversification is adopted resulting in the selection of more superior strains through the accumulation of...

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Main Authors: Prakash, V., Tan, Y. H.
Format: Article
Language:English
Published: Malaysian Society of Applied Biology 1977
Online Access:http://psasir.upm.edu.my/id/eprint/33884/1/Mutation%20breeding%20of%20microbial%20strain.pdf
http://psasir.upm.edu.my/id/eprint/33884/
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spelling my.upm.eprints.338842015-10-28T23:32:09Z http://psasir.upm.edu.my/id/eprint/33884/ Mutation breeding of microbial strain (Streptomyces fradiae) for increased antibiotic yield Prakash, V. Tan, Y. H. A strain improvement programme for higher yield has been attempted for a local anti- biotic-producing strain, Streptomyces fradiae. An empirical approach of sequential mutation-selection and rediversification is adopted resulting in the selection of more superior strains through the accumulation of favourable minor genes. A stepwise selection over six phases have been successfully tried. Each phase consists of a two-stage selection; selection in the first stage being made on colonies which have arisen from spores subjected to mutagenic treatment and selection in the second stage is a reselection of the selected first stage mutants. In the first stage selection, the use of the Agar Piece Method (Ichikawa, 1971) is effective and resulted in the saving of valuable screening time. In the second stage selection the selection is performed on shaken flask cultures. The two-stage test compromises between time saved (in the first stage) and reliability. Ultraviolet radiation and N-methyl-N'-rutro- N-nitrosoguanidine are effective mutagens and the combined use of both is more superior to the single use of one in the breeding programme. The effect of selection is expectedly greatest in the beginning of the programme. The two main limitations of the selection procedure are the ignorance of the particular gene(s) responsible for oversynthesis of the antibiotic and the laborious nature of the procedure. However as an empirical approach, it is still overall, a very effective method of microbial breeding. Malaysian Society of Applied Biology 1977 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/33884/1/Mutation%20breeding%20of%20microbial%20strain.pdf Prakash, V. and Tan, Y. H. (1977) Mutation breeding of microbial strain (Streptomyces fradiae) for increased antibiotic yield. Malaysian Applied Biololgy, 6 (2). pp. 129-140. ISSN 0126-8643
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A strain improvement programme for higher yield has been attempted for a local anti- biotic-producing strain, Streptomyces fradiae. An empirical approach of sequential mutation-selection and rediversification is adopted resulting in the selection of more superior strains through the accumulation of favourable minor genes. A stepwise selection over six phases have been successfully tried. Each phase consists of a two-stage selection; selection in the first stage being made on colonies which have arisen from spores subjected to mutagenic treatment and selection in the second stage is a reselection of the selected first stage mutants. In the first stage selection, the use of the Agar Piece Method (Ichikawa, 1971) is effective and resulted in the saving of valuable screening time. In the second stage selection the selection is performed on shaken flask cultures. The two-stage test compromises between time saved (in the first stage) and reliability. Ultraviolet radiation and N-methyl-N'-rutro- N-nitrosoguanidine are effective mutagens and the combined use of both is more superior to the single use of one in the breeding programme. The effect of selection is expectedly greatest in the beginning of the programme. The two main limitations of the selection procedure are the ignorance of the particular gene(s) responsible for oversynthesis of the antibiotic and the laborious nature of the procedure. However as an empirical approach, it is still overall, a very effective method of microbial breeding.
format Article
author Prakash, V.
Tan, Y. H.
spellingShingle Prakash, V.
Tan, Y. H.
Mutation breeding of microbial strain (Streptomyces fradiae) for increased antibiotic yield
author_facet Prakash, V.
Tan, Y. H.
author_sort Prakash, V.
title Mutation breeding of microbial strain (Streptomyces fradiae) for increased antibiotic yield
title_short Mutation breeding of microbial strain (Streptomyces fradiae) for increased antibiotic yield
title_full Mutation breeding of microbial strain (Streptomyces fradiae) for increased antibiotic yield
title_fullStr Mutation breeding of microbial strain (Streptomyces fradiae) for increased antibiotic yield
title_full_unstemmed Mutation breeding of microbial strain (Streptomyces fradiae) for increased antibiotic yield
title_sort mutation breeding of microbial strain (streptomyces fradiae) for increased antibiotic yield
publisher Malaysian Society of Applied Biology
publishDate 1977
url http://psasir.upm.edu.my/id/eprint/33884/1/Mutation%20breeding%20of%20microbial%20strain.pdf
http://psasir.upm.edu.my/id/eprint/33884/
_version_ 1643830997970583552
score 13.211869

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