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Genetic structure of giant honeybee (Apis dorsata fabricius) population in Peninsular Malaysia

The knowledge on the genetic structure of Apis dorsata is needed for effective management and conservation strategies of this species in Malaysia, especially to prevent over-harvesting of honey. The main objective of this research was to estimate genetic structure and relatedness of Malaysian A.dors...

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Main Author: Sahebzadeh, Najmeh
Format: Thesis
Language:English
Published: 2012
Online Access:http://psasir.upm.edu.my/id/eprint/33478/1/FP%202012%2046R.pdf
http://psasir.upm.edu.my/id/eprint/33478/
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spelling my.upm.eprints.334782015-03-31T00:59:08Z http://psasir.upm.edu.my/id/eprint/33478/ Genetic structure of giant honeybee (Apis dorsata fabricius) population in Peninsular Malaysia Sahebzadeh, Najmeh The knowledge on the genetic structure of Apis dorsata is needed for effective management and conservation strategies of this species in Malaysia, especially to prevent over-harvesting of honey. The main objective of this research was to estimate genetic structure and relatedness of Malaysian A.dorsata populations (2009-2010) from Terengganu and Kedah using 30 DNA microsatellites. The results showed that all the loci and sample size were informative for measuring the genetic variation in A.dorsata aggregations. The results of genetic structure revealed that there was no significant deviation Hardy-Weinberg equilibrium and linkage disequilibrium (P>0.05) within and between aggregations. Population differentiation coefficient (FST) indicated a great level (FST>0.15) within five aggregations and a moderate level (FST=0.095) of genetic differentiation within an aggregation of Terengganu. A moderate level (FST>0.07) of FST was detected within Pedu Lake aggregations. The values of FST between Terengganu 2009 aggregations showed no genetic differentiation (P>0.21), whereas genetic differentiation was detected (P<0.001) between Terengganu 2010. Pair-wise of FST values between the aggregations, before (2009) and after (2010) migration, showed that two of three aggregations from Terengganu returned to their nest sites. A same result was obtained by Pedu Lake aggregations. Analysis of molecular variance (AMOVA) indicated that the nests within each aggregation were genetically and statistically distinct, as a result of the restricted drone congregation areas (DCA) of each aggregation. Amount of misclassification at assignment tests was in line with the results of FST and AMOVA, indicating two of three aggregations returned to the natal site afetr the migration (2010). The intra-colony pedigree relatedness values within aggregations showed a low level of genetic relatedness (0.29). Furthermore, genetic relatedness analyses confirmed that the queens within the aggregations were genetically different. However, presence of full and half siblings between same aggregations site before and after migration supported a probability that a particular aggregation returns to its natal site after the migration if there are available empty tree timbers. The finding of an appreciable level of aggregation fidelity indicated that a comprehensive study with a large number of aggregations throughout the region would be necessary to provide concrete proof for this idea. Finally, the genetic structure and relatedness among the solitary nests from Terengganu, 2010 showed only one nest pair were headed away by same queens. However, the finding of an appreciable level of intercolonial relatedness between the nests of A.dorsata sheds light on a larger sample size of solitary nests which were found to provide concrete proof for that particular hypothesis. This finding suggests that the formidable anthropogenic pressures may change the genetic structure of A.dorsata population in Malaysia, which still needs to be researched on in the future. 2012-12 Thesis NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/33478/1/FP%202012%2046R.pdf Sahebzadeh, Najmeh (2012) Genetic structure of giant honeybee (Apis dorsata fabricius) population in Peninsular Malaysia. PhD thesis, Universiti Putra Malaysia.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The knowledge on the genetic structure of Apis dorsata is needed for effective management and conservation strategies of this species in Malaysia, especially to prevent over-harvesting of honey. The main objective of this research was to estimate genetic structure and relatedness of Malaysian A.dorsata populations (2009-2010) from Terengganu and Kedah using 30 DNA microsatellites. The results showed that all the loci and sample size were informative for measuring the genetic variation in A.dorsata aggregations. The results of genetic structure revealed that there was no significant deviation Hardy-Weinberg equilibrium and linkage disequilibrium (P>0.05) within and between aggregations. Population differentiation coefficient (FST) indicated a great level (FST>0.15) within five aggregations and a moderate level (FST=0.095) of genetic differentiation within an aggregation of Terengganu. A moderate level (FST>0.07) of FST was detected within Pedu Lake aggregations. The values of FST between Terengganu 2009 aggregations showed no genetic differentiation (P>0.21), whereas genetic differentiation was detected (P<0.001) between Terengganu 2010. Pair-wise of FST values between the aggregations, before (2009) and after (2010) migration, showed that two of three aggregations from Terengganu returned to their nest sites. A same result was obtained by Pedu Lake aggregations. Analysis of molecular variance (AMOVA) indicated that the nests within each aggregation were genetically and statistically distinct, as a result of the restricted drone congregation areas (DCA) of each aggregation. Amount of misclassification at assignment tests was in line with the results of FST and AMOVA, indicating two of three aggregations returned to the natal site afetr the migration (2010). The intra-colony pedigree relatedness values within aggregations showed a low level of genetic relatedness (0.29). Furthermore, genetic relatedness analyses confirmed that the queens within the aggregations were genetically different. However, presence of full and half siblings between same aggregations site before and after migration supported a probability that a particular aggregation returns to its natal site after the migration if there are available empty tree timbers. The finding of an appreciable level of aggregation fidelity indicated that a comprehensive study with a large number of aggregations throughout the region would be necessary to provide concrete proof for this idea. Finally, the genetic structure and relatedness among the solitary nests from Terengganu, 2010 showed only one nest pair were headed away by same queens. However, the finding of an appreciable level of intercolonial relatedness between the nests of A.dorsata sheds light on a larger sample size of solitary nests which were found to provide concrete proof for that particular hypothesis. This finding suggests that the formidable anthropogenic pressures may change the genetic structure of A.dorsata population in Malaysia, which still needs to be researched on in the future.
format Thesis
author Sahebzadeh, Najmeh
spellingShingle Sahebzadeh, Najmeh
Genetic structure of giant honeybee (Apis dorsata fabricius) population in Peninsular Malaysia
author_facet Sahebzadeh, Najmeh
author_sort Sahebzadeh, Najmeh
title Genetic structure of giant honeybee (Apis dorsata fabricius) population in Peninsular Malaysia
title_short Genetic structure of giant honeybee (Apis dorsata fabricius) population in Peninsular Malaysia
title_full Genetic structure of giant honeybee (Apis dorsata fabricius) population in Peninsular Malaysia
title_fullStr Genetic structure of giant honeybee (Apis dorsata fabricius) population in Peninsular Malaysia
title_full_unstemmed Genetic structure of giant honeybee (Apis dorsata fabricius) population in Peninsular Malaysia
title_sort genetic structure of giant honeybee (apis dorsata fabricius) population in peninsular malaysia
publishDate 2012
url http://psasir.upm.edu.my/id/eprint/33478/1/FP%202012%2046R.pdf
http://psasir.upm.edu.my/id/eprint/33478/
_version_ 1643830887033339904
score 13.211869

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