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A method for producing a biopolymer

The present invention relates to a method for producing a biopolymer, comprising the steps of providing a culture medium comprising a carbon source, a nitrogen source, and a plurality of mineral sources; inoculating the culture medium with a Aspergillus flavus with an inoculum size in a range of 0.2...

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Main Authors: Idris, Azni, Aljuboori, Ahmad H. Rajab, Abdullah, Norhafizah, Mohamad, Rosfarizan
Format: Patent
Published: 2011
Online Access:http://psasir.upm.edu.my/id/eprint/33129/
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spelling my.upm.eprints.331292014-12-24T01:26:04Z http://psasir.upm.edu.my/id/eprint/33129/ A method for producing a biopolymer Idris, Azni Aljuboori, Ahmad H. Rajab Abdullah, Norhafizah Mohamad, Rosfarizan The present invention relates to a method for producing a biopolymer, comprising the steps of providing a culture medium comprising a carbon source, a nitrogen source, and a plurality of mineral sources; inoculating the culture medium with a Aspergillus flavus with an inoculum size in a range of 0.2 to 10 % by volume; incubating the culture medium; filtering the culture medium, thereby separating the Aspergillus flavus and the biopolymer; purifying the biopolymer; wherein the carbon source to nitrogen source is in a molar ratio of 5:1, pH of the culture medium is 5 to 9, the temperature is 25 to 40ºC, inoculum size is 0.2 to 2 % by volume, shaking speed is 50 to 200 rpm, and culture time is 12 to 60 hours. A biopolymer produced having a molecular weight in a range of 2.466x104 to 2.68 x104 Dalton, a plurality of functional groups, a plurality of chemical elements, a sugar, a protein, a minimum flocculating efficiency of 90 % in a pH range of 3 to 7 and a temperature range of 10 to 100ºC. 2011-11-02 Patent NonPeerReviewed Azni Idris (2011) A method for producing a biopolymer. PI2011005282.
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
description The present invention relates to a method for producing a biopolymer, comprising the steps of providing a culture medium comprising a carbon source, a nitrogen source, and a plurality of mineral sources; inoculating the culture medium with a Aspergillus flavus with an inoculum size in a range of 0.2 to 10 % by volume; incubating the culture medium; filtering the culture medium, thereby separating the Aspergillus flavus and the biopolymer; purifying the biopolymer; wherein the carbon source to nitrogen source is in a molar ratio of 5:1, pH of the culture medium is 5 to 9, the temperature is 25 to 40ºC, inoculum size is 0.2 to 2 % by volume, shaking speed is 50 to 200 rpm, and culture time is 12 to 60 hours. A biopolymer produced having a molecular weight in a range of 2.466x104 to 2.68 x104 Dalton, a plurality of functional groups, a plurality of chemical elements, a sugar, a protein, a minimum flocculating efficiency of 90 % in a pH range of 3 to 7 and a temperature range of 10 to 100ºC.
format Patent
author Idris, Azni
Aljuboori, Ahmad H. Rajab
Abdullah, Norhafizah
Mohamad, Rosfarizan
spellingShingle Idris, Azni
Aljuboori, Ahmad H. Rajab
Abdullah, Norhafizah
Mohamad, Rosfarizan
A method for producing a biopolymer
author_facet Idris, Azni
Aljuboori, Ahmad H. Rajab
Abdullah, Norhafizah
Mohamad, Rosfarizan
author_sort Idris, Azni
title A method for producing a biopolymer
title_short A method for producing a biopolymer
title_full A method for producing a biopolymer
title_fullStr A method for producing a biopolymer
title_full_unstemmed A method for producing a biopolymer
title_sort method for producing a biopolymer
publishDate 2011
url http://psasir.upm.edu.my/id/eprint/33129/
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score 13.211869

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