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Molecular approach in avian sexing using cheek cells.

This study was carried out to determine the sex of birds through the DNA extracted from cheek cells using polymerase chain reaction (PCR) technique. Sterile swabs were used to collect cheek cells from 39 adults of various psittacine species as a source of DNA. A pair of Eclectus parrots (Eclectus...

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Main Authors: Wan Mahamood, Wan Aini, Abu, Jalila, Omar, Abdul Rahman
Format: Conference or Workshop Item
Language:English
English
Published: 2010
Online Access:http://psasir.upm.edu.my/id/eprint/31392/1/Proceedings-49.pdf
http://psasir.upm.edu.my/id/eprint/31392/
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spelling my.upm.eprints.313922014-06-16T08:02:31Z http://psasir.upm.edu.my/id/eprint/31392/ Molecular approach in avian sexing using cheek cells. Wan Mahamood, Wan Aini Abu, Jalila Omar, Abdul Rahman This study was carried out to determine the sex of birds through the DNA extracted from cheek cells using polymerase chain reaction (PCR) technique. Sterile swabs were used to collect cheek cells from 39 adults of various psittacine species as a source of DNA. A pair of Eclectus parrots (Eclectus roratus), a sexual dimorphism species was used as a control group. Various quantities of DNA were extracted from the buccal samples by the conventional DNA extraction method. A set of primers, P2 and P8, was designed for the study. The positive control samples were tested with the primers that will bind to CHD W and CHD Z genes, in the chromosome Z and W of the birds. The PCR products of P2 and P8 showed a single band (300 bps) for male and double bands (300 bps and 400 bps) for female birds. Based on the PCR results, of the 39 samples tested, there were 15 males (39%), 12 females (31%) and 12 unknown (12%) sex. This study shows that avian cheek cells contain DNA and can be used as a tool for sex or gender determination for avian species. 2010-01-05 Conference or Workshop Item PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/31392/1/Proceedings-49.pdf Wan Mahamood, Wan Aini and Abu, Jalila and Omar, Abdul Rahman (2010) Molecular approach in avian sexing using cheek cells. In: 5th Proceedings of the Seminar on Veterinary Sciences, 5-8 Jan. 2010, Serdang, Selangor. . English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description This study was carried out to determine the sex of birds through the DNA extracted from cheek cells using polymerase chain reaction (PCR) technique. Sterile swabs were used to collect cheek cells from 39 adults of various psittacine species as a source of DNA. A pair of Eclectus parrots (Eclectus roratus), a sexual dimorphism species was used as a control group. Various quantities of DNA were extracted from the buccal samples by the conventional DNA extraction method. A set of primers, P2 and P8, was designed for the study. The positive control samples were tested with the primers that will bind to CHD W and CHD Z genes, in the chromosome Z and W of the birds. The PCR products of P2 and P8 showed a single band (300 bps) for male and double bands (300 bps and 400 bps) for female birds. Based on the PCR results, of the 39 samples tested, there were 15 males (39%), 12 females (31%) and 12 unknown (12%) sex. This study shows that avian cheek cells contain DNA and can be used as a tool for sex or gender determination for avian species.
format Conference or Workshop Item
author Wan Mahamood, Wan Aini
Abu, Jalila
Omar, Abdul Rahman
spellingShingle Wan Mahamood, Wan Aini
Abu, Jalila
Omar, Abdul Rahman
Molecular approach in avian sexing using cheek cells.
author_facet Wan Mahamood, Wan Aini
Abu, Jalila
Omar, Abdul Rahman
author_sort Wan Mahamood, Wan Aini
title Molecular approach in avian sexing using cheek cells.
title_short Molecular approach in avian sexing using cheek cells.
title_full Molecular approach in avian sexing using cheek cells.
title_fullStr Molecular approach in avian sexing using cheek cells.
title_full_unstemmed Molecular approach in avian sexing using cheek cells.
title_sort molecular approach in avian sexing using cheek cells.
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/31392/1/Proceedings-49.pdf
http://psasir.upm.edu.my/id/eprint/31392/
_version_ 1643830313458073600
score 13.211869

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