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Correlation between serological tests and identification of Brucella melitensis in goats.

Brucellosis is a specific contagious disease of humans and animals caused by bacteria of the Brucella group, and caprine brucellosis is primarily caused by Brucella melitensis. In Malaysia, serological tests such as Rose Bengal Plate Test (RBPT) and Complement Fixation Test (CFT) are most widely use...

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Main Authors: Abd Jalil, Mohd Syazuwan, Saad, Mohd Zamri, Shamsudin, Shahirudin
Format: Conference or Workshop Item
Language:English
Published: 2010
Online Access:http://psasir.upm.edu.my/id/eprint/30922/1/Proceedings-28.pdf
http://psasir.upm.edu.my/id/eprint/30922/
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spelling my.upm.eprints.309222014-11-06T10:02:22Z http://psasir.upm.edu.my/id/eprint/30922/ Correlation between serological tests and identification of Brucella melitensis in goats. Abd Jalil, Mohd Syazuwan Saad, Mohd Zamri Shamsudin, Shahirudin Brucellosis is a specific contagious disease of humans and animals caused by bacteria of the Brucella group, and caprine brucellosis is primarily caused by Brucella melitensis. In Malaysia, serological tests such as Rose Bengal Plate Test (RBPT) and Complement Fixation Test (CFT) are most widely used in diagnosis of brucellosis in small ruminants, while isolation and Polymerase Chain Reaction (PCR) provide valuable information regarding the presence of Brucella organism. This research was performed to evaluate and determine the correlation between serological tests (RBPT and CFT) and presence of Brucella melitensis in seropositive goats. Ninety-nine goat sera were collected from five goat farms and subjected to RBPT. Out of ninety-nine goat sera tested, 11(11%) were positive. They were then subjected to CFT. All RBPT seropositive goats were then killed and tissues such as supramammary lymph nodes, uterus, mammary glands and spleen were taken for isolation and identification using culture and PCR technique. The results showed that there was no correlation between the serological tests and the presence of B.melitensis in goats. However, there was strong correlation between RBPT and CFT (r = 0.83, P< 0.01). Therefore, neither RBPT nor CFT is suitable to be used as confirmatory diagnostic tool for control of caprine brucellosis. 2010-01-05 Conference or Workshop Item NonPeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30922/1/Proceedings-28.pdf Abd Jalil, Mohd Syazuwan and Saad, Mohd Zamri and Shamsudin, Shahirudin (2010) Correlation between serological tests and identification of Brucella melitensis in goats. In: 5th Seminar on Veterinary Sciences, 5-8 Jan. 2010, Serdang, Selangor. .
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Brucellosis is a specific contagious disease of humans and animals caused by bacteria of the Brucella group, and caprine brucellosis is primarily caused by Brucella melitensis. In Malaysia, serological tests such as Rose Bengal Plate Test (RBPT) and Complement Fixation Test (CFT) are most widely used in diagnosis of brucellosis in small ruminants, while isolation and Polymerase Chain Reaction (PCR) provide valuable information regarding the presence of Brucella organism. This research was performed to evaluate and determine the correlation between serological tests (RBPT and CFT) and presence of Brucella melitensis in seropositive goats. Ninety-nine goat sera were collected from five goat farms and subjected to RBPT. Out of ninety-nine goat sera tested, 11(11%) were positive. They were then subjected to CFT. All RBPT seropositive goats were then killed and tissues such as supramammary lymph nodes, uterus, mammary glands and spleen were taken for isolation and identification using culture and PCR technique. The results showed that there was no correlation between the serological tests and the presence of B.melitensis in goats. However, there was strong correlation between RBPT and CFT (r = 0.83, P< 0.01). Therefore, neither RBPT nor CFT is suitable to be used as confirmatory diagnostic tool for control of caprine brucellosis.
format Conference or Workshop Item
author Abd Jalil, Mohd Syazuwan
Saad, Mohd Zamri
Shamsudin, Shahirudin
spellingShingle Abd Jalil, Mohd Syazuwan
Saad, Mohd Zamri
Shamsudin, Shahirudin
Correlation between serological tests and identification of Brucella melitensis in goats.
author_facet Abd Jalil, Mohd Syazuwan
Saad, Mohd Zamri
Shamsudin, Shahirudin
author_sort Abd Jalil, Mohd Syazuwan
title Correlation between serological tests and identification of Brucella melitensis in goats.
title_short Correlation between serological tests and identification of Brucella melitensis in goats.
title_full Correlation between serological tests and identification of Brucella melitensis in goats.
title_fullStr Correlation between serological tests and identification of Brucella melitensis in goats.
title_full_unstemmed Correlation between serological tests and identification of Brucella melitensis in goats.
title_sort correlation between serological tests and identification of brucella melitensis in goats.
publishDate 2010
url http://psasir.upm.edu.my/id/eprint/30922/1/Proceedings-28.pdf
http://psasir.upm.edu.my/id/eprint/30922/
_version_ 1643830206059773952
score 13.211869

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