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Analysis of partial AZFc deletions in Malaysian infertile male subjects

Complete deletions in the AZF (a, b, and c) sub-regions of the Y-chromosome have been shown to contribute to unexplained male infertility. However, the role of partial AZFc deletions in male infertility remains to be verified. Three types of partial AZFc deletions have been identified. They are gr/g...

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Main Authors: Almeamar, Hussein Ali, Ramachandran, Vasudevan, Ismail, Patimah, Nadkarni, Prashan, Fawzi, Nora
Format: Article
Language:English
Published: Informa Healthcare 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30733/1/Analysis%20of%20partial%20AZFc%20deletions%20in%20Malaysian%20infertile%20male%20subjects.pdf
http://psasir.upm.edu.my/id/eprint/30733/
http://informahealthcare.com/doi/abs/10.3109/19396368.2012.748851
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spelling my.upm.eprints.307332015-09-08T02:59:24Z http://psasir.upm.edu.my/id/eprint/30733/ Analysis of partial AZFc deletions in Malaysian infertile male subjects Almeamar, Hussein Ali Ramachandran, Vasudevan Ismail, Patimah Nadkarni, Prashan Fawzi, Nora Complete deletions in the AZF (a, b, and c) sub-regions of the Y-chromosome have been shown to contribute to unexplained male infertility. However, the role of partial AZFc deletions in male infertility remains to be verified. Three types of partial AZFc deletions have been identified. They are gr/gr, b1/b3, and b2/b3 deletions. A recent meta-analysis showed that ethnic and geographical factors might contribute to the association of partial AZFc deletions with male infertility. This study analyzed the association of partial AZFc deletions in Malaysian infertile males. Fifty two oligozoospermic infertile males and 63 fertile controls were recruited to this study. Screening for partial AZFc deletions was done using the two sequence-tagged sites approach (SY1291 and SY1191) which were analyzed using both the conventional PCR gel-electrophoresis and the high resolution melt, HRM method. Gr/gr deletions were found in 11.53% of the cases and 9.52% of the controls (p = 0.725). A B2/b3 deletion was found in one of the cases (p = 0.269). No B1/b3 deletions were identified in this study. The results of HRM analysis were consistent with those obtained using the conventional PCR gel-electrophoresis method. The HRM analysis was highly repeatable (95% limit of agreement was -0.0879 to 0.0871 for SY1191 melting temperature readings). In conclusion, our study showed that partial AZFc deletions were not associated with male infertility in Malaysian subjects. HRM analysis was a reliable, repeatable, fast, cost-effective, and semi-automated method which can be used for screening of partial AZFc deletions. Informa Healthcare 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30733/1/Analysis%20of%20partial%20AZFc%20deletions%20in%20Malaysian%20infertile%20male%20subjects.pdf Almeamar, Hussein Ali and Ramachandran, Vasudevan and Ismail, Patimah and Nadkarni, Prashan and Fawzi, Nora (2013) Analysis of partial AZFc deletions in Malaysian infertile male subjects. System Biology in Reproductive Medicine, 59 (2). pp. 99-107. ISSN 1939-6368; ESSN: 1939-6376 http://informahealthcare.com/doi/abs/10.3109/19396368.2012.748851 10.3109/19396368.2012.748851
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Complete deletions in the AZF (a, b, and c) sub-regions of the Y-chromosome have been shown to contribute to unexplained male infertility. However, the role of partial AZFc deletions in male infertility remains to be verified. Three types of partial AZFc deletions have been identified. They are gr/gr, b1/b3, and b2/b3 deletions. A recent meta-analysis showed that ethnic and geographical factors might contribute to the association of partial AZFc deletions with male infertility. This study analyzed the association of partial AZFc deletions in Malaysian infertile males. Fifty two oligozoospermic infertile males and 63 fertile controls were recruited to this study. Screening for partial AZFc deletions was done using the two sequence-tagged sites approach (SY1291 and SY1191) which were analyzed using both the conventional PCR gel-electrophoresis and the high resolution melt, HRM method. Gr/gr deletions were found in 11.53% of the cases and 9.52% of the controls (p = 0.725). A B2/b3 deletion was found in one of the cases (p = 0.269). No B1/b3 deletions were identified in this study. The results of HRM analysis were consistent with those obtained using the conventional PCR gel-electrophoresis method. The HRM analysis was highly repeatable (95% limit of agreement was -0.0879 to 0.0871 for SY1191 melting temperature readings). In conclusion, our study showed that partial AZFc deletions were not associated with male infertility in Malaysian subjects. HRM analysis was a reliable, repeatable, fast, cost-effective, and semi-automated method which can be used for screening of partial AZFc deletions.
format Article
author Almeamar, Hussein Ali
Ramachandran, Vasudevan
Ismail, Patimah
Nadkarni, Prashan
Fawzi, Nora
spellingShingle Almeamar, Hussein Ali
Ramachandran, Vasudevan
Ismail, Patimah
Nadkarni, Prashan
Fawzi, Nora
Analysis of partial AZFc deletions in Malaysian infertile male subjects
author_facet Almeamar, Hussein Ali
Ramachandran, Vasudevan
Ismail, Patimah
Nadkarni, Prashan
Fawzi, Nora
author_sort Almeamar, Hussein Ali
title Analysis of partial AZFc deletions in Malaysian infertile male subjects
title_short Analysis of partial AZFc deletions in Malaysian infertile male subjects
title_full Analysis of partial AZFc deletions in Malaysian infertile male subjects
title_fullStr Analysis of partial AZFc deletions in Malaysian infertile male subjects
title_full_unstemmed Analysis of partial AZFc deletions in Malaysian infertile male subjects
title_sort analysis of partial azfc deletions in malaysian infertile male subjects
publisher Informa Healthcare
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/30733/1/Analysis%20of%20partial%20AZFc%20deletions%20in%20Malaysian%20infertile%20male%20subjects.pdf
http://psasir.upm.edu.my/id/eprint/30733/
http://informahealthcare.com/doi/abs/10.3109/19396368.2012.748851
_version_ 1643830147639410688
score 13.211869

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