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Cytotoxic activity of kenaf seed oils from supercritical carbon dioxide fluid extraction towards human colorectal cancer (HT29) cell lines

Kenaf (Hibiscus cannabinus) from the family Malvaceae, is a valuable fiber plant native to India and Africa and is currently planted as the fourth commercial crop in Malaysia. Kenaf seed oil contains alpha-linolenic acid, phytosterol such as β-sitosterol, vitamin E, and other antioxidants with chemo...

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Main Authors: Abd Ghafar, Siti Aisyah, Ismail, Maznah, Saiful Yazan, Latifah, Fakurazi, Sharida, Ismail, Norsharina, Kim, Wei Chan, Md. Tahir, Paridah
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30722/1/Cytotoxic%20activity%20of%20kenaf%20seed%20oils%20from%20supercritical%20carbon%20dioxide%20fluid%20extraction%20towards%20human%20colorectal%20cancer.pdf
http://psasir.upm.edu.my/id/eprint/30722/
http://dx.doi.org/10.1155/2013/549705
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spelling my.upm.eprints.307222016-02-15T07:24:26Z http://psasir.upm.edu.my/id/eprint/30722/ Cytotoxic activity of kenaf seed oils from supercritical carbon dioxide fluid extraction towards human colorectal cancer (HT29) cell lines Abd Ghafar, Siti Aisyah Ismail, Maznah Saiful Yazan, Latifah Fakurazi, Sharida Ismail, Norsharina Kim, Wei Chan Md. Tahir, Paridah Kenaf (Hibiscus cannabinus) from the family Malvaceae, is a valuable fiber plant native to India and Africa and is currently planted as the fourth commercial crop in Malaysia. Kenaf seed oil contains alpha-linolenic acid, phytosterol such as β-sitosterol, vitamin E, and other antioxidants with chemopreventive properties. Kenaf seeds oil (KSO) was from supercritical carbon dioxide extraction fluid (SFE) at 9 different permutations of parameters based on range of pressures from 200 to 600 bars and temperature from 40 to 80°C. They were 200/40, 200/60, 200/80, 400/40, 400/60, 400/80, 600/40, 600/60, and 600/80. Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. KSO-SFE at 600/40 showed the strongest cytotoxicity towards HT29 with IC50 of 200 µg/mL. The IC50 for NIH/3T3 was not detected even at highest concentration employed. Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. Further apoptosis induction was confirmed by Annexin V/PI and AO/PI staining. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30722/1/Cytotoxic%20activity%20of%20kenaf%20seed%20oils%20from%20supercritical%20carbon%20dioxide%20fluid%20extraction%20towards%20human%20colorectal%20cancer.pdf Abd Ghafar, Siti Aisyah and Ismail, Maznah and Saiful Yazan, Latifah and Fakurazi, Sharida and Ismail, Norsharina and Kim, Wei Chan and Md. Tahir, Paridah (2013) Cytotoxic activity of kenaf seed oils from supercritical carbon dioxide fluid extraction towards human colorectal cancer (HT29) cell lines. Evidence-Based Complementary and Alternative Medicine, 2013. art. no. 549705. pp. 1-8. ISSN 1741-427X; ESSN: 1741-4288 http://dx.doi.org/10.1155/2013/549705 10.1155/2013/549705
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Kenaf (Hibiscus cannabinus) from the family Malvaceae, is a valuable fiber plant native to India and Africa and is currently planted as the fourth commercial crop in Malaysia. Kenaf seed oil contains alpha-linolenic acid, phytosterol such as β-sitosterol, vitamin E, and other antioxidants with chemopreventive properties. Kenaf seeds oil (KSO) was from supercritical carbon dioxide extraction fluid (SFE) at 9 different permutations of parameters based on range of pressures from 200 to 600 bars and temperature from 40 to 80°C. They were 200/40, 200/60, 200/80, 400/40, 400/60, 400/80, 600/40, 600/60, and 600/80. Extraction from 9 parameters of KSO-SFE was screened for cytotoxicity towards human colorectal cancer cell lines (HT29) and mouse embryonic fibroblast (NIH/3T3) cell lines using MTS assay. KSO-SFE at 600/40 showed the strongest cytotoxicity towards HT29 with IC50 of 200 µg/mL. The IC50 for NIH/3T3 was not detected even at highest concentration employed. Cell cycle analysis showed a significant increase in the accumulation of KSO-SFE-treated cells at sub-G1 phase, indicating the induction of apoptosis by KSO-SFE. Further apoptosis induction was confirmed by Annexin V/PI and AO/PI staining.
format Article
author Abd Ghafar, Siti Aisyah
Ismail, Maznah
Saiful Yazan, Latifah
Fakurazi, Sharida
Ismail, Norsharina
Kim, Wei Chan
Md. Tahir, Paridah
spellingShingle Abd Ghafar, Siti Aisyah
Ismail, Maznah
Saiful Yazan, Latifah
Fakurazi, Sharida
Ismail, Norsharina
Kim, Wei Chan
Md. Tahir, Paridah
Cytotoxic activity of kenaf seed oils from supercritical carbon dioxide fluid extraction towards human colorectal cancer (HT29) cell lines
author_facet Abd Ghafar, Siti Aisyah
Ismail, Maznah
Saiful Yazan, Latifah
Fakurazi, Sharida
Ismail, Norsharina
Kim, Wei Chan
Md. Tahir, Paridah
author_sort Abd Ghafar, Siti Aisyah
title Cytotoxic activity of kenaf seed oils from supercritical carbon dioxide fluid extraction towards human colorectal cancer (HT29) cell lines
title_short Cytotoxic activity of kenaf seed oils from supercritical carbon dioxide fluid extraction towards human colorectal cancer (HT29) cell lines
title_full Cytotoxic activity of kenaf seed oils from supercritical carbon dioxide fluid extraction towards human colorectal cancer (HT29) cell lines
title_fullStr Cytotoxic activity of kenaf seed oils from supercritical carbon dioxide fluid extraction towards human colorectal cancer (HT29) cell lines
title_full_unstemmed Cytotoxic activity of kenaf seed oils from supercritical carbon dioxide fluid extraction towards human colorectal cancer (HT29) cell lines
title_sort cytotoxic activity of kenaf seed oils from supercritical carbon dioxide fluid extraction towards human colorectal cancer (ht29) cell lines
publisher Hindawi Publishing Corporation
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/30722/1/Cytotoxic%20activity%20of%20kenaf%20seed%20oils%20from%20supercritical%20carbon%20dioxide%20fluid%20extraction%20towards%20human%20colorectal%20cancer.pdf
http://psasir.upm.edu.my/id/eprint/30722/
http://dx.doi.org/10.1155/2013/549705
_version_ 1643830144259850240
score 13.211869

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