Staff View: Quantification of Escherichia coli O157:H7 in organic vegetables and chickens.

Quantification of Escherichia coli O157:H7 in organic vegetables and chickens.

The organic foods’ market is becoming one of the rapidly growing sections in agricultural economies in the world. During the last two decades, food-borne outbreaks associated with fresh produce have rapidly increased. E. coli O57:H7, the caustic agent of acute hemorrhagic diarrhea and abdominal...

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Main Authors:	Chang, Wei San, Afsah Hejri, Leili, Rukayadi, Yaya, Khatib, Alfi, Lye, Ying Ling, Loo, Yuet Ying, Mohd Shahril, Najwa, Soopna, Puspanadan, Kuan, Chee Hao, Goh, Sur Guat, Tang, John Yew Huat, Nakaguchi, Yoshitsugu, Nishibuchi, Mitsuaki, Radu, Son
Format:	Article
Language:	English English
Published:	Faculty of Food Science and Technology, Universiti Putra Malaysia 2013
Online Access:	http://psasir.upm.edu.my/id/eprint/30512/1/30512.pdf http://psasir.upm.edu.my/id/eprint/30512/7/Quantification%20of%20Escherichia%20coli%20O157.pdf http://psasir.upm.edu.my/id/eprint/30512/ http://www.ifrj.upm.edu.my/ifrj-2013-20-issue-2.html
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spelling	my.upm.eprints.305122015-09-15T01:25:46Z http://psasir.upm.edu.my/id/eprint/30512/ Quantification of Escherichia coli O157:H7 in organic vegetables and chickens. Chang, Wei San Afsah Hejri, Leili Rukayadi, Yaya Khatib, Alfi Lye, Ying Ling Loo, Yuet Ying Mohd Shahril, Najwa Soopna, Puspanadan Kuan, Chee Hao Goh, Sur Guat Tang, John Yew Huat Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son The organic foods’ market is becoming one of the rapidly growing sections in agricultural economies in the world. During the last two decades, food-borne outbreaks associated with fresh produce have rapidly increased. E. coli O57:H7, the caustic agent of acute hemorrhagic diarrhea and abdominal cramps, is mainly associated with meat and poultry product outbreaks but frequent outbreaks linked to the consumption of vegetables have been reported. The aim of this study was to investigate prevalence of E. coli O157:H7 in some organic foods. A total of 230 organic food samples including four-winged bean, tomato, white radish, red cabbage, chinese cabbage, lettuce, cucumber and chicken form retailed groceries and supermarkets in Malaysia were investigated. Low prevalence of E. coli O157:H7 was detected in organic vegetables and chickens. The estimated quantity of E. coli O157:H7 in all samples ranged from <3 to >2400 MPN/g. The overall MPN/g estimate of E. coli O157:H7 in the samples from organic groceries was higher than supermarket with the maximum of >2400 MPN/g. Most of the samples from supermarket showed a minimum of <3 MPN/g. The specific target genes produced amplicons of 259 bp and 625 bp after PCR amplification and E. coli O157:H7 was detected in 5.2% of total organic samples. Prevalence of E. coli O157: H7 in organic foods from groceries (8.8%) was particularly higher than supermarkets (1.0%). The highest prevalence of E. coli O157:H7 was observed in organic chickens (40%) purchased from groceries followed by four-winged bean (10%) and white radish (3.3%). Faculty of Food Science and Technology, Universiti Putra Malaysia 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30512/1/30512.pdf application/pdf en http://psasir.upm.edu.my/id/eprint/30512/7/Quantification%20of%20Escherichia%20coli%20O157.pdf Chang, Wei San and Afsah Hejri, Leili and Rukayadi, Yaya and Khatib, Alfi and Lye, Ying Ling and Loo, Yuet Ying and Mohd Shahril, Najwa and Soopna, Puspanadan and Kuan, Chee Hao and Goh, Sur Guat and Tang, John Yew Huat and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2013) Quantification of Escherichia coli O157:H7 in organic vegetables and chickens. International Food Research Journal, 20 (2). pp. 1023-1029. ISSN 1985-4668; ESSN: 2231-7546 http://www.ifrj.upm.edu.my/ifrj-2013-20-issue-2.html
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English English
description	The organic foods’ market is becoming one of the rapidly growing sections in agricultural economies in the world. During the last two decades, food-borne outbreaks associated with fresh produce have rapidly increased. E. coli O57:H7, the caustic agent of acute hemorrhagic diarrhea and abdominal cramps, is mainly associated with meat and poultry product outbreaks but frequent outbreaks linked to the consumption of vegetables have been reported. The aim of this study was to investigate prevalence of E. coli O157:H7 in some organic foods. A total of 230 organic food samples including four-winged bean, tomato, white radish, red cabbage, chinese cabbage, lettuce, cucumber and chicken form retailed groceries and supermarkets in Malaysia were investigated. Low prevalence of E. coli O157:H7 was detected in organic vegetables and chickens. The estimated quantity of E. coli O157:H7 in all samples ranged from <3 to >2400 MPN/g. The overall MPN/g estimate of E. coli O157:H7 in the samples from organic groceries was higher than supermarket with the maximum of >2400 MPN/g. Most of the samples from supermarket showed a minimum of <3 MPN/g. The specific target genes produced amplicons of 259 bp and 625 bp after PCR amplification and E. coli O157:H7 was detected in 5.2% of total organic samples. Prevalence of E. coli O157: H7 in organic foods from groceries (8.8%) was particularly higher than supermarkets (1.0%). The highest prevalence of E. coli O157:H7 was observed in organic chickens (40%) purchased from groceries followed by four-winged bean (10%) and white radish (3.3%).
format	Article
author	Chang, Wei San Afsah Hejri, Leili Rukayadi, Yaya Khatib, Alfi Lye, Ying Ling Loo, Yuet Ying Mohd Shahril, Najwa Soopna, Puspanadan Kuan, Chee Hao Goh, Sur Guat Tang, John Yew Huat Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son
spellingShingle	Chang, Wei San Afsah Hejri, Leili Rukayadi, Yaya Khatib, Alfi Lye, Ying Ling Loo, Yuet Ying Mohd Shahril, Najwa Soopna, Puspanadan Kuan, Chee Hao Goh, Sur Guat Tang, John Yew Huat Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son Quantification of Escherichia coli O157:H7 in organic vegetables and chickens.
author_facet	Chang, Wei San Afsah Hejri, Leili Rukayadi, Yaya Khatib, Alfi Lye, Ying Ling Loo, Yuet Ying Mohd Shahril, Najwa Soopna, Puspanadan Kuan, Chee Hao Goh, Sur Guat Tang, John Yew Huat Nakaguchi, Yoshitsugu Nishibuchi, Mitsuaki Radu, Son
author_sort	Chang, Wei San
title	Quantification of Escherichia coli O157:H7 in organic vegetables and chickens.
title_short	Quantification of Escherichia coli O157:H7 in organic vegetables and chickens.
title_full	Quantification of Escherichia coli O157:H7 in organic vegetables and chickens.
title_fullStr	Quantification of Escherichia coli O157:H7 in organic vegetables and chickens.
title_full_unstemmed	Quantification of Escherichia coli O157:H7 in organic vegetables and chickens.
title_sort	quantification of escherichia coli o157:h7 in organic vegetables and chickens.
publisher	Faculty of Food Science and Technology, Universiti Putra Malaysia
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/30512/1/30512.pdf http://psasir.upm.edu.my/id/eprint/30512/7/Quantification%20of%20Escherichia%20coli%20O157.pdf http://psasir.upm.edu.my/id/eprint/30512/ http://www.ifrj.upm.edu.my/ifrj-2013-20-issue-2.html
_version_	1643830081051688960
score	13.211869

Quantification of Escherichia coli O157:H7 in organic vegetables and chickens.

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