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Anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants.

Different parts of Acacia nilotica (L.) Delile, Calotropis procera (Aiton) W.T. Aiton, Adhatoda vasica Nees, Fagoniaar abica L. and Casuarina equisetifolia L. are traditionally used in folk medicine for the treatment of a variety of common ailments like nausea, cold, cough, asthma, fevers, diarrhea,...

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Main Authors: Amin, Muhammad, Anwar, Farooq, Naz, Fauqia, Mehmood, Tahir, Saari, Nazamid
Format: Article
Language:English
Published: MDPI 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30503/1/Anti%20helicobacter.pdf
http://psasir.upm.edu.my/id/eprint/30503/
http://www.mdpi.com/1420-3049/18/2/2135
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spelling my.upm.eprints.305032015-09-08T03:35:52Z http://psasir.upm.edu.my/id/eprint/30503/ Anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants. Amin, Muhammad Anwar, Farooq Naz, Fauqia Mehmood, Tahir Saari, Nazamid Different parts of Acacia nilotica (L.) Delile, Calotropis procera (Aiton) W.T. Aiton, Adhatoda vasica Nees, Fagoniaar abica L. and Casuarina equisetifolia L. are traditionally used in folk medicine for the treatment of a variety of common ailments like nausea, cold, cough, asthma, fevers, diarrhea, sore throat, swelling, etc. The present study was aimed to evaluate the anti-Helicobacter pylori and urease inhibition activities of extracts produced from the above selected medicinal plants native to Soon Valley (home to an old civilization) in the Punjab province of Pakistan. Methanol, acetone and water extracts of the plants were evaluated for anti-bacterial activity against thirty four clinical isolates and two reference strains of H. pylori. Minimum inhibitory concentrations (MICs) of the extracts were determined using the agar dilution method and compared with some standard antibiotics like amoxicillin (AMX), clarithromycin (CLA), tetracycline (TET) and metronidazole (MNZ), used in the triple therapy for H. pylori eradication. H. pylori urease inhibition activity of the extracts was assessed by the phenol red method, wherein, Lineweaver-Burk plots were used to determine Michaelis-Menten constants for elucidating the mechanism of inhibition. Methanol and acetone extracts from Acacia nilotica and Calotropis procera exhibited stronger anti-H. pylori activity than MNZ, almost comparable activity with TET, but were found to be less potent than AMX and CLT. The rest of the extracts exhibited lower activity than the standard antibiotics used in this study. In the H. pylori urease inhibitory assay, methanol and acetone extracts of Acacia nilotica and Calotropis procera showed significant inhibition. Lineweaver-Burk plots indicated a competitive mechanism for extract of Acacia nilotica, whereas extract of Calotropis procera exhibited a mixed type of inhibition. MDPI 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30503/1/Anti%20helicobacter.pdf Amin, Muhammad and Anwar, Farooq and Naz, Fauqia and Mehmood, Tahir and Saari, Nazamid (2013) Anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants. Molecules, 18 (2). pp. 2135-2149. ISSN 1420-3049 http://www.mdpi.com/1420-3049/18/2/2135 10.3390/molecules18022135
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Different parts of Acacia nilotica (L.) Delile, Calotropis procera (Aiton) W.T. Aiton, Adhatoda vasica Nees, Fagoniaar abica L. and Casuarina equisetifolia L. are traditionally used in folk medicine for the treatment of a variety of common ailments like nausea, cold, cough, asthma, fevers, diarrhea, sore throat, swelling, etc. The present study was aimed to evaluate the anti-Helicobacter pylori and urease inhibition activities of extracts produced from the above selected medicinal plants native to Soon Valley (home to an old civilization) in the Punjab province of Pakistan. Methanol, acetone and water extracts of the plants were evaluated for anti-bacterial activity against thirty four clinical isolates and two reference strains of H. pylori. Minimum inhibitory concentrations (MICs) of the extracts were determined using the agar dilution method and compared with some standard antibiotics like amoxicillin (AMX), clarithromycin (CLA), tetracycline (TET) and metronidazole (MNZ), used in the triple therapy for H. pylori eradication. H. pylori urease inhibition activity of the extracts was assessed by the phenol red method, wherein, Lineweaver-Burk plots were used to determine Michaelis-Menten constants for elucidating the mechanism of inhibition. Methanol and acetone extracts from Acacia nilotica and Calotropis procera exhibited stronger anti-H. pylori activity than MNZ, almost comparable activity with TET, but were found to be less potent than AMX and CLT. The rest of the extracts exhibited lower activity than the standard antibiotics used in this study. In the H. pylori urease inhibitory assay, methanol and acetone extracts of Acacia nilotica and Calotropis procera showed significant inhibition. Lineweaver-Burk plots indicated a competitive mechanism for extract of Acacia nilotica, whereas extract of Calotropis procera exhibited a mixed type of inhibition.
format Article
author Amin, Muhammad
Anwar, Farooq
Naz, Fauqia
Mehmood, Tahir
Saari, Nazamid
spellingShingle Amin, Muhammad
Anwar, Farooq
Naz, Fauqia
Mehmood, Tahir
Saari, Nazamid
Anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants.
author_facet Amin, Muhammad
Anwar, Farooq
Naz, Fauqia
Mehmood, Tahir
Saari, Nazamid
author_sort Amin, Muhammad
title Anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants.
title_short Anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants.
title_full Anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants.
title_fullStr Anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants.
title_full_unstemmed Anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants.
title_sort anti-helicobacter pylori and urease inhibition activities of some traditional medicinal plants.
publisher MDPI
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/30503/1/Anti%20helicobacter.pdf
http://psasir.upm.edu.my/id/eprint/30503/
http://www.mdpi.com/1420-3049/18/2/2135
_version_ 1643830078380965888
score 13.211869

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