Cover Image

Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor

Lipase-catalyzed production of triethanolamine-based esterquat by esterification of oleic acid (OA) with triethanolamine (TEA) in n-hexane was performed in 2 L stirred-tank reactor. A set of experiments was designed by central composite design to process modeling and statistically evaluate the findi...

Full description

Saved in:
Bibliographic Details
Main Authors: Masoumi, Hamid Reza Fard, Basri, Mahiran, Kassim, Anuar, Abdullah, Dzulkefly Kuang, Abdollahi, Yadollah, Abd Gani, Siti Salwa, Rezaee, Malahat
Format: Article
Language:English
English
Published: Hindawi Publishing Corporation 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30240/1/Statistical%20optimization%20of%20process%20parameters%20for%20lipase.pdf
http://psasir.upm.edu.my/id/eprint/30240/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.30240
record_format eprints
spelling my.upm.eprints.302402015-10-08T07:53:36Z http://psasir.upm.edu.my/id/eprint/30240/ Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor Masoumi, Hamid Reza Fard Basri, Mahiran Kassim, Anuar Abdullah, Dzulkefly Kuang Abdollahi, Yadollah Abd Gani, Siti Salwa Rezaee, Malahat Lipase-catalyzed production of triethanolamine-based esterquat by esterification of oleic acid (OA) with triethanolamine (TEA) in n-hexane was performed in 2 L stirred-tank reactor. A set of experiments was designed by central composite design to process modeling and statistically evaluate the findings. Five independent process variables, including enzyme amount, reaction time, reaction temperature, substrates molar ratio of OA to TEA, and agitation speed, were studied under the given conditions designed by Design Expert software. Experimental data were examined for normality test before data processing stage and skewness and kurtosis indices were determined. The mathematical model developed was found to be adequate and statistically accurate to predict the optimum conversion of product. Response surface methodology with central composite design gave the best performance in this study, and the methodology as a whole has been proven to be adequate for the design and optimization of the enzymatic process. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30240/1/Statistical%20optimization%20of%20process%20parameters%20for%20lipase.pdf Masoumi, Hamid Reza Fard and Basri, Mahiran and Kassim, Anuar and Abdullah, Dzulkefly Kuang and Abdollahi, Yadollah and Abd Gani, Siti Salwa and Rezaee, Malahat (2013) Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor. The Scientific World Journal, 2013. art. no. 962083. pp. 1-9. ISSN 2356-6140; ESSN: 1537-744X 10.1155/2013/962083 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Lipase-catalyzed production of triethanolamine-based esterquat by esterification of oleic acid (OA) with triethanolamine (TEA) in n-hexane was performed in 2 L stirred-tank reactor. A set of experiments was designed by central composite design to process modeling and statistically evaluate the findings. Five independent process variables, including enzyme amount, reaction time, reaction temperature, substrates molar ratio of OA to TEA, and agitation speed, were studied under the given conditions designed by Design Expert software. Experimental data were examined for normality test before data processing stage and skewness and kurtosis indices were determined. The mathematical model developed was found to be adequate and statistically accurate to predict the optimum conversion of product. Response surface methodology with central composite design gave the best performance in this study, and the methodology as a whole has been proven to be adequate for the design and optimization of the enzymatic process.
format Article
author Masoumi, Hamid Reza Fard
Basri, Mahiran
Kassim, Anuar
Abdullah, Dzulkefly Kuang
Abdollahi, Yadollah
Abd Gani, Siti Salwa
Rezaee, Malahat
spellingShingle Masoumi, Hamid Reza Fard
Basri, Mahiran
Kassim, Anuar
Abdullah, Dzulkefly Kuang
Abdollahi, Yadollah
Abd Gani, Siti Salwa
Rezaee, Malahat
Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor
author_facet Masoumi, Hamid Reza Fard
Basri, Mahiran
Kassim, Anuar
Abdullah, Dzulkefly Kuang
Abdollahi, Yadollah
Abd Gani, Siti Salwa
Rezaee, Malahat
author_sort Masoumi, Hamid Reza Fard
title Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor
title_short Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor
title_full Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor
title_fullStr Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor
title_full_unstemmed Statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor
title_sort statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor
publisher Hindawi Publishing Corporation
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/30240/1/Statistical%20optimization%20of%20process%20parameters%20for%20lipase.pdf
http://psasir.upm.edu.my/id/eprint/30240/
_version_ 1643829999180972032
score 13.211869

Similar Items