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Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies

Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multil...

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Main Authors: Yee, Elsie Yoke Sim, Zainuddin, Zainal Zahari, Ismail, Ahmad, Yap, Chee Kong, Tan, Soon Guan
Format: Article
Language:English
English
Published: Springer 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30206/1/Identification%20of%20hybrids%20of%20painted%20and%20milky%20storks%20using%20FTA%20card.pdf
http://psasir.upm.edu.my/id/eprint/30206/
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spelling my.upm.eprints.302062015-10-23T03:34:01Z http://psasir.upm.edu.my/id/eprint/30206/ Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies Yee, Elsie Yoke Sim Zainuddin, Zainal Zahari Ismail, Ahmad Yap, Chee Kong Tan, Soon Guan Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patterns that provided two sets of genetic data, one for each of the two stork species and another for the hybrids. It also revealed that large DNA fragments (3,000 bp) could be amplified from blood collected on FTA cards. When the results of each individual bird’s DNA fingerprint were compared with plumage characters, the hybrids were found to express a range of intermediate phenotypic traits of the pure breeds with no dominant plumage characteristic from either parental species. Springer 2013-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30206/1/Identification%20of%20hybrids%20of%20painted%20and%20milky%20storks%20using%20FTA%20card.pdf Yee, Elsie Yoke Sim and Zainuddin, Zainal Zahari and Ismail, Ahmad and Yap, Chee Kong and Tan, Soon Guan (2013) Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies. Biochemical Genetics, 51 (9-10). pp. 789-799. ISSN 0006-2928; ESSN: 1573-4927 10.1007/s10528-013-9607-8 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patterns that provided two sets of genetic data, one for each of the two stork species and another for the hybrids. It also revealed that large DNA fragments (3,000 bp) could be amplified from blood collected on FTA cards. When the results of each individual bird’s DNA fingerprint were compared with plumage characters, the hybrids were found to express a range of intermediate phenotypic traits of the pure breeds with no dominant plumage characteristic from either parental species.
format Article
author Yee, Elsie Yoke Sim
Zainuddin, Zainal Zahari
Ismail, Ahmad
Yap, Chee Kong
Tan, Soon Guan
spellingShingle Yee, Elsie Yoke Sim
Zainuddin, Zainal Zahari
Ismail, Ahmad
Yap, Chee Kong
Tan, Soon Guan
Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies
author_facet Yee, Elsie Yoke Sim
Zainuddin, Zainal Zahari
Ismail, Ahmad
Yap, Chee Kong
Tan, Soon Guan
author_sort Yee, Elsie Yoke Sim
title Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies
title_short Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies
title_full Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies
title_fullStr Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies
title_full_unstemmed Identification of hybrids of painted and milky storks using FTA card-collected blood, molecular markers, and morphologies
title_sort identification of hybrids of painted and milky storks using fta card-collected blood, molecular markers, and morphologies
publisher Springer
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/30206/1/Identification%20of%20hybrids%20of%20painted%20and%20milky%20storks%20using%20FTA%20card.pdf
http://psasir.upm.edu.my/id/eprint/30206/
_version_ 1643829989080039424
score 13.211869

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