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Phenolic contents, antioxidant and cytotoxic activities of Elaeocarpus floribundus Blume

Elaeocarpus floribundus is higher plant that has been used as traditional medicine for treating several diseases. There is no previous report on phytochemicals and bioactivity studies of this species. In this investigation, triterpenoids friedelin, epifriedelanol and β-sitosterol were isolated f...

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Main Authors: Utami, Rahayu, Khalid, Nurhasniza, Sukari, Mohd Aspollah, Rahmani, Mawardi, Abdul, Ahmad Bustamam, Dachriyanus,
Format: Article
Language:English
English
Published: University of Karachi 2013
Online Access:http://psasir.upm.edu.my/id/eprint/30036/2/Phenolic%20contents%2C%20antioxidant%20and%20cytotoxic%20activities%20of%20Elaeocarpus%20floribundus%20Blume.pdf
http://psasir.upm.edu.my/id/eprint/30036/
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spelling my.upm.eprints.300362015-10-06T01:31:16Z http://psasir.upm.edu.my/id/eprint/30036/ Phenolic contents, antioxidant and cytotoxic activities of Elaeocarpus floribundus Blume Utami, Rahayu Khalid, Nurhasniza Sukari, Mohd Aspollah Rahmani, Mawardi Abdul, Ahmad Bustamam Dachriyanus, Elaeocarpus floribundus is higher plant that has been used as traditional medicine for treating several diseases. There is no previous report on phytochemicals and bioactivity studies of this species. In this investigation, triterpenoids friedelin, epifriedelanol and β-sitosterol were isolated from its leaves and stem bark. Determination of total phenolic content of methanolic extract of leaves and stem bark was carried out using Folin-Ciocalteu reagent. All extracts and isolated compounds were subjected to screening of antioxidant activity using DPPH free radical scavenging method and cytotoxic activities by MTT assay towards human T4 lymphoblastoid (CEM-SS) and human cervical (HeLa) cancer cells. In the total phenolic content determination, methanolic extract of leaves gave higher value of 503.08±16.71 mg GAE/g DW than stem bark with value of 161.5±24.81 mg GAE/g DW. Polar extracts of leaves and stem bark possessed promising antioxidant activity with methanol extract of stem bark exhibited strongest activity with IC50 value of 7.36±0.01 µg/ml. In the cytotoxic activity assay, only chloroform extract of leaves showed significant activity with IC50 value of 25.6±0.06 µg/ml against CEM-SS cancer cell, while friedelin and epifriedelanol were found to be active against the two cancer cells with IC50 values ranging from 3.54 to 11.45 µg/ml. University of Karachi 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/30036/2/Phenolic%20contents%2C%20antioxidant%20and%20cytotoxic%20activities%20of%20Elaeocarpus%20floribundus%20Blume.pdf Utami, Rahayu and Khalid, Nurhasniza and Sukari, Mohd Aspollah and Rahmani, Mawardi and Abdul, Ahmad Bustamam and Dachriyanus, (2013) Phenolic contents, antioxidant and cytotoxic activities of Elaeocarpus floribundus Blume. Pakistan Journal of Pharmaceutical Sciences, 26 (2). pp. 245-250. ISSN 1011-601X English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Elaeocarpus floribundus is higher plant that has been used as traditional medicine for treating several diseases. There is no previous report on phytochemicals and bioactivity studies of this species. In this investigation, triterpenoids friedelin, epifriedelanol and β-sitosterol were isolated from its leaves and stem bark. Determination of total phenolic content of methanolic extract of leaves and stem bark was carried out using Folin-Ciocalteu reagent. All extracts and isolated compounds were subjected to screening of antioxidant activity using DPPH free radical scavenging method and cytotoxic activities by MTT assay towards human T4 lymphoblastoid (CEM-SS) and human cervical (HeLa) cancer cells. In the total phenolic content determination, methanolic extract of leaves gave higher value of 503.08±16.71 mg GAE/g DW than stem bark with value of 161.5±24.81 mg GAE/g DW. Polar extracts of leaves and stem bark possessed promising antioxidant activity with methanol extract of stem bark exhibited strongest activity with IC50 value of 7.36±0.01 µg/ml. In the cytotoxic activity assay, only chloroform extract of leaves showed significant activity with IC50 value of 25.6±0.06 µg/ml against CEM-SS cancer cell, while friedelin and epifriedelanol were found to be active against the two cancer cells with IC50 values ranging from 3.54 to 11.45 µg/ml.
format Article
author Utami, Rahayu
Khalid, Nurhasniza
Sukari, Mohd Aspollah
Rahmani, Mawardi
Abdul, Ahmad Bustamam
Dachriyanus,
spellingShingle Utami, Rahayu
Khalid, Nurhasniza
Sukari, Mohd Aspollah
Rahmani, Mawardi
Abdul, Ahmad Bustamam
Dachriyanus,
Phenolic contents, antioxidant and cytotoxic activities of Elaeocarpus floribundus Blume
author_facet Utami, Rahayu
Khalid, Nurhasniza
Sukari, Mohd Aspollah
Rahmani, Mawardi
Abdul, Ahmad Bustamam
Dachriyanus,
author_sort Utami, Rahayu
title Phenolic contents, antioxidant and cytotoxic activities of Elaeocarpus floribundus Blume
title_short Phenolic contents, antioxidant and cytotoxic activities of Elaeocarpus floribundus Blume
title_full Phenolic contents, antioxidant and cytotoxic activities of Elaeocarpus floribundus Blume
title_fullStr Phenolic contents, antioxidant and cytotoxic activities of Elaeocarpus floribundus Blume
title_full_unstemmed Phenolic contents, antioxidant and cytotoxic activities of Elaeocarpus floribundus Blume
title_sort phenolic contents, antioxidant and cytotoxic activities of elaeocarpus floribundus blume
publisher University of Karachi
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/30036/2/Phenolic%20contents%2C%20antioxidant%20and%20cytotoxic%20activities%20of%20Elaeocarpus%20floribundus%20Blume.pdf
http://psasir.upm.edu.my/id/eprint/30036/
_version_ 1643829937956716544
score 13.211869

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