Cover Image

Clinico-pathological features in mice following oral exposure to Pasteurella multocida B: 2

Haemorrhagic septicaemia (HS) is a major cause of losses to livestock production in many countries around the world. In Malaysia, more specifically, the disease yet remains a major constraint to the national industry. However, the pathogenesis of haemorrhagic septicaemia is another scenario in which...

Full description

Saved in:
Bibliographic Details
Main Authors: Abdullah, Faez Firdaus Jesse, Ahmad Affandi, Syahirah, Osman, Abdinasir Yusuf, Adamu, Lawan, Saad, Mohd Zamri, Haron, Abd Wahid, Omar, Abdul Rahman, Sabri, Jasni, Saharee, Abdul Aziz
Format: Article
Language:English
Published: International Organization of Scientific Research 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29854/1/Clinico.pdf
http://psasir.upm.edu.my/id/eprint/29854/
http://www.iosrjournals.org/iosr-javs/pages/v3i4.html
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.29854
record_format eprints
spelling my.upm.eprints.298542015-09-07T04:23:17Z http://psasir.upm.edu.my/id/eprint/29854/ Clinico-pathological features in mice following oral exposure to Pasteurella multocida B: 2 Abdullah, Faez Firdaus Jesse Ahmad Affandi, Syahirah Osman, Abdinasir Yusuf Adamu, Lawan Saad, Mohd Zamri Haron, Abd Wahid Omar, Abdul Rahman Sabri, Jasni Saharee, Abdul Aziz Haemorrhagic septicaemia (HS) is a major cause of losses to livestock production in many countries around the world. In Malaysia, more specifically, the disease yet remains a major constraint to the national industry. However, the pathogenesis of haemorrhagic septicaemia is another scenario in which the limitations still exists. Thus, the present paper provides more information on the pathogenicity and host response dynamics in a mouse model. Our study of experimental nature manipulates P. multocida serotype B:2, the bacterium responsible for the disease in Asia. In this study, sixteen mice (n=16) were divided into two groups (A & B) of 8 mice each group. Animals in group A were inoculated orally with 1.0 ml 109 cfu/ml of P. multocida type B while mice in group 2 were challenged orally with 1.0 ml of phosphate buffer saline (PBS). The animals were observed for clinical signs for 5 days. The mice showing severe signs and surviving mice after 5 days of post- inoculation were euthanized using cervical dislocation approach and the organs such as heart, lung, kidney, stomach, spleen, colon and small intestine were collected for microscopic examinations. The result indicated that mice inoculated with the Pasteurella multocida showed significant (p<0.05) severe clinical signs compared to control group.These clinical signs ranged from mild to severe in which most of individuals infected with Pasteurella multocida showed moderate to severe clinical signs of ruffled hair, laboured breathing, eye discharge and responsiveness with mean levels of 2.13±0.64, 1.88±0.99, 1.50±1.20 and 1.88±0.99 respectively in comparison to the control group. Moreover, mortality rate was recorded between 24 to 50 h post-inoculation in the group that challenged with Pasteurella multocida type B: 2. Microscopically, the extent of visceral tissue damages due to the infection was scored. The interested parameters included pulmonary oedema, presence of inflammatory cells, haemorrhage and necrosis. Of these parameters, animals in infected group showed significant (p<0.05) differences in all most all visceral organs. Lungs, liver and kidney were, in particular, the most predominantly affected tissues. Therefore, oral inoculation of P. multocida type B in mice showed significant clinical response and cellular changes. International Organization of Scientific Research 2013-05 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29854/1/Clinico.pdf Abdullah, Faez Firdaus Jesse and Ahmad Affandi, Syahirah and Osman, Abdinasir Yusuf and Adamu, Lawan and Saad, Mohd Zamri and Haron, Abd Wahid and Omar, Abdul Rahman and Sabri, Jasni and Saharee, Abdul Aziz (2013) Clinico-pathological features in mice following oral exposure to Pasteurella multocida B: 2. IOSR Journal of Agriculture and Veterinary Science, 3 (4). pp. 35-39. ISSN 2319-2372; ESSN: 2319-2380 http://www.iosrjournals.org/iosr-javs/pages/v3i4.html 10.9790/2380-0343539
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Haemorrhagic septicaemia (HS) is a major cause of losses to livestock production in many countries around the world. In Malaysia, more specifically, the disease yet remains a major constraint to the national industry. However, the pathogenesis of haemorrhagic septicaemia is another scenario in which the limitations still exists. Thus, the present paper provides more information on the pathogenicity and host response dynamics in a mouse model. Our study of experimental nature manipulates P. multocida serotype B:2, the bacterium responsible for the disease in Asia. In this study, sixteen mice (n=16) were divided into two groups (A & B) of 8 mice each group. Animals in group A were inoculated orally with 1.0 ml 109 cfu/ml of P. multocida type B while mice in group 2 were challenged orally with 1.0 ml of phosphate buffer saline (PBS). The animals were observed for clinical signs for 5 days. The mice showing severe signs and surviving mice after 5 days of post- inoculation were euthanized using cervical dislocation approach and the organs such as heart, lung, kidney, stomach, spleen, colon and small intestine were collected for microscopic examinations. The result indicated that mice inoculated with the Pasteurella multocida showed significant (p<0.05) severe clinical signs compared to control group.These clinical signs ranged from mild to severe in which most of individuals infected with Pasteurella multocida showed moderate to severe clinical signs of ruffled hair, laboured breathing, eye discharge and responsiveness with mean levels of 2.13±0.64, 1.88±0.99, 1.50±1.20 and 1.88±0.99 respectively in comparison to the control group. Moreover, mortality rate was recorded between 24 to 50 h post-inoculation in the group that challenged with Pasteurella multocida type B: 2. Microscopically, the extent of visceral tissue damages due to the infection was scored. The interested parameters included pulmonary oedema, presence of inflammatory cells, haemorrhage and necrosis. Of these parameters, animals in infected group showed significant (p<0.05) differences in all most all visceral organs. Lungs, liver and kidney were, in particular, the most predominantly affected tissues. Therefore, oral inoculation of P. multocida type B in mice showed significant clinical response and cellular changes.
format Article
author Abdullah, Faez Firdaus Jesse
Ahmad Affandi, Syahirah
Osman, Abdinasir Yusuf
Adamu, Lawan
Saad, Mohd Zamri
Haron, Abd Wahid
Omar, Abdul Rahman
Sabri, Jasni
Saharee, Abdul Aziz
spellingShingle Abdullah, Faez Firdaus Jesse
Ahmad Affandi, Syahirah
Osman, Abdinasir Yusuf
Adamu, Lawan
Saad, Mohd Zamri
Haron, Abd Wahid
Omar, Abdul Rahman
Sabri, Jasni
Saharee, Abdul Aziz
Clinico-pathological features in mice following oral exposure to Pasteurella multocida B: 2
author_facet Abdullah, Faez Firdaus Jesse
Ahmad Affandi, Syahirah
Osman, Abdinasir Yusuf
Adamu, Lawan
Saad, Mohd Zamri
Haron, Abd Wahid
Omar, Abdul Rahman
Sabri, Jasni
Saharee, Abdul Aziz
author_sort Abdullah, Faez Firdaus Jesse
title Clinico-pathological features in mice following oral exposure to Pasteurella multocida B: 2
title_short Clinico-pathological features in mice following oral exposure to Pasteurella multocida B: 2
title_full Clinico-pathological features in mice following oral exposure to Pasteurella multocida B: 2
title_fullStr Clinico-pathological features in mice following oral exposure to Pasteurella multocida B: 2
title_full_unstemmed Clinico-pathological features in mice following oral exposure to Pasteurella multocida B: 2
title_sort clinico-pathological features in mice following oral exposure to pasteurella multocida b: 2
publisher International Organization of Scientific Research
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29854/1/Clinico.pdf
http://psasir.upm.edu.my/id/eprint/29854/
http://www.iosrjournals.org/iosr-javs/pages/v3i4.html
_version_ 1643829884414328832
score 13.211869

Similar Items