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Insulin secreted from genetically engineered intestinal cells reduces blood glucose levels in diabetic mice

Poorly controlled diabetes mellitus can result in serious complications. Gene therapy is increasingly being considered as an alternative approach to treat diabetes, because of its ability to induce physiological insulin secretion and it allows patients to escape insulin injections. The properties of...

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Main Authors: Rasouli, Mina, Allaudin, Zeenathul Nazariah, Omar, Abdul Rahman, Ahmad, Zalinah
Format: Article
Language:English
Published: Bentham Science Publishers 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29849/1/Insulin%20secreted%20from%20genetically%20engineered%20intestinal%20cells%20reduces%20blood%20glucose%20levels%20in%20diabetic%20mice.pdf
http://psasir.upm.edu.my/id/eprint/29849/
http://www.eurekaselect.com/110938/article
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spelling my.upm.eprints.298492015-10-30T02:50:27Z http://psasir.upm.edu.my/id/eprint/29849/ Insulin secreted from genetically engineered intestinal cells reduces blood glucose levels in diabetic mice Rasouli, Mina Allaudin, Zeenathul Nazariah Omar, Abdul Rahman Ahmad, Zalinah Poorly controlled diabetes mellitus can result in serious complications. Gene therapy is increasingly being considered as an alternative approach to treat diabetes, because of its ability to induce physiological insulin secretion and it allows patients to escape insulin injections. The properties of gut K and L-cells, including glucose sensitivity, the ability to process insulin and a regulated secretion pathway support their use as surrogate β-cells. Previous in vitro studies have provided sufficient evidence supporting the use of these cells for gene therapy studies. Therefore, we examined the ability of K and L-cells to produce insulin in diabetic mice. Chitosan nanoparticles were used to transfer the insulin gene into intestinal cells via oral administration. The efficiency of chitosan as a gene vehicle was investigated through the use of reporter gene. Insulin mRNA and protein expression levels were measured by RT-PCR and ELISA, respectively. Blood glucose testing revealed that this treatment reduced glucose levels in diabetic mice. The decrease in blood glucose level in the first week of treatment was greater in mice with K-cell specific insulin expression compared with mice with L-cellspecific insulin expression. These results indicate that inducing insulin secretion in K-cells conferred a quicker response to gene therapy. Bentham Science Publishers 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29849/1/Insulin%20secreted%20from%20genetically%20engineered%20intestinal%20cells%20reduces%20blood%20glucose%20levels%20in%20diabetic%20mice.pdf Rasouli, Mina and Allaudin, Zeenathul Nazariah and Omar, Abdul Rahman and Ahmad, Zalinah (2013) Insulin secreted from genetically engineered intestinal cells reduces blood glucose levels in diabetic mice. Current Gene Therapy, 13 (4). pp. 229-239. ISSN 1566-5232; ESSN: 1875-5631 http://www.eurekaselect.com/110938/article 10.2174/15665232113139990002
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Poorly controlled diabetes mellitus can result in serious complications. Gene therapy is increasingly being considered as an alternative approach to treat diabetes, because of its ability to induce physiological insulin secretion and it allows patients to escape insulin injections. The properties of gut K and L-cells, including glucose sensitivity, the ability to process insulin and a regulated secretion pathway support their use as surrogate β-cells. Previous in vitro studies have provided sufficient evidence supporting the use of these cells for gene therapy studies. Therefore, we examined the ability of K and L-cells to produce insulin in diabetic mice. Chitosan nanoparticles were used to transfer the insulin gene into intestinal cells via oral administration. The efficiency of chitosan as a gene vehicle was investigated through the use of reporter gene. Insulin mRNA and protein expression levels were measured by RT-PCR and ELISA, respectively. Blood glucose testing revealed that this treatment reduced glucose levels in diabetic mice. The decrease in blood glucose level in the first week of treatment was greater in mice with K-cell specific insulin expression compared with mice with L-cellspecific insulin expression. These results indicate that inducing insulin secretion in K-cells conferred a quicker response to gene therapy.
format Article
author Rasouli, Mina
Allaudin, Zeenathul Nazariah
Omar, Abdul Rahman
Ahmad, Zalinah
spellingShingle Rasouli, Mina
Allaudin, Zeenathul Nazariah
Omar, Abdul Rahman
Ahmad, Zalinah
Insulin secreted from genetically engineered intestinal cells reduces blood glucose levels in diabetic mice
author_facet Rasouli, Mina
Allaudin, Zeenathul Nazariah
Omar, Abdul Rahman
Ahmad, Zalinah
author_sort Rasouli, Mina
title Insulin secreted from genetically engineered intestinal cells reduces blood glucose levels in diabetic mice
title_short Insulin secreted from genetically engineered intestinal cells reduces blood glucose levels in diabetic mice
title_full Insulin secreted from genetically engineered intestinal cells reduces blood glucose levels in diabetic mice
title_fullStr Insulin secreted from genetically engineered intestinal cells reduces blood glucose levels in diabetic mice
title_full_unstemmed Insulin secreted from genetically engineered intestinal cells reduces blood glucose levels in diabetic mice
title_sort insulin secreted from genetically engineered intestinal cells reduces blood glucose levels in diabetic mice
publisher Bentham Science Publishers
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29849/1/Insulin%20secreted%20from%20genetically%20engineered%20intestinal%20cells%20reduces%20blood%20glucose%20levels%20in%20diabetic%20mice.pdf
http://psasir.upm.edu.my/id/eprint/29849/
http://www.eurekaselect.com/110938/article
_version_ 1643829882893893632
score 13.211869

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