Staff View: Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection

Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection

Fifteen sequences with stop codons have been obtained in the course of standard methicillin-resistant Staphylococcus aureus (MRSA) spa typing. In nine of those sequences, stop codons occurred due to nonsense G-T and A-T transversions. G-T transversions would appear to be frequent in the spa gene, mo...

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Main Authors:	Khrustalev, Vladislav Victorovich, Ghaznavi Rad, Ehsanollah, Neela, Vasantha Kumari, Shamsudin, Mariana Nor, Amouzandeh-Nobaveh, Alireza, Barkovsky, Eugene Victorovich
Format:	Article
Published:	Elsevier 2013
Online Access:	http://psasir.upm.edu.my/id/eprint/29802/
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spelling	my.upm.eprints.298022016-02-19T02:24:16Z http://psasir.upm.edu.my/id/eprint/29802/ Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection Khrustalev, Vladislav Victorovich Ghaznavi Rad, Ehsanollah Neela, Vasantha Kumari Shamsudin, Mariana Nor Amouzandeh-Nobaveh, Alireza Barkovsky, Eugene Victorovich Fifteen sequences with stop codons have been obtained in the course of standard methicillin-resistant Staphylococcus aureus (MRSA) spa typing. In nine of those sequences, stop codons occurred due to nonsense G-T and A-T transversions. G-T transversions would appear to be frequent in the spa gene, mostly due to symmetric mutational AT-pressure in the whole S. aureus genome and due to replication-associated mutational pressure characteristic of lagging strands of the "chromosome". A-T transversions would appear to be frequent in the spa gene mostly due to transcription-associated mutational pressure. Relative to other S. aureus genes, short repeats in spa are enriched by nonsense sites for G-T and A-T transversions; the probability of being nonsense for A-T transversion is high in that part of spa coding region. 13 out of 15 (87%) of the sequences with stop codons were obtained from strains isolated from patients with generalized S. aureus infection. Truncation of spa at its C-terminus is predicted to result in a protein that possesses functional IgG binding domains unable to be linked to the cell wall. This is discussed in light of the known fact that extracellular spa is a strong virulence factor involved in immune evasion. Elsevier 2013-11 Article PeerReviewed Khrustalev, Vladislav Victorovich and Ghaznavi Rad, Ehsanollah and Neela, Vasantha Kumari and Shamsudin, Mariana Nor and Amouzandeh-Nobaveh, Alireza and Barkovsky, Eugene Victorovich (2013) Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection. Research in Microbiology, 164 (9). pp. 913-922. ISSN 0923-2508; ESSN: 1769-7123 10.1016/j.resmic.2013.07.003
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description	Fifteen sequences with stop codons have been obtained in the course of standard methicillin-resistant Staphylococcus aureus (MRSA) spa typing. In nine of those sequences, stop codons occurred due to nonsense G-T and A-T transversions. G-T transversions would appear to be frequent in the spa gene, mostly due to symmetric mutational AT-pressure in the whole S. aureus genome and due to replication-associated mutational pressure characteristic of lagging strands of the "chromosome". A-T transversions would appear to be frequent in the spa gene mostly due to transcription-associated mutational pressure. Relative to other S. aureus genes, short repeats in spa are enriched by nonsense sites for G-T and A-T transversions; the probability of being nonsense for A-T transversion is high in that part of spa coding region. 13 out of 15 (87%) of the sequences with stop codons were obtained from strains isolated from patients with generalized S. aureus infection. Truncation of spa at its C-terminus is predicted to result in a protein that possesses functional IgG binding domains unable to be linked to the cell wall. This is discussed in light of the known fact that extracellular spa is a strong virulence factor involved in immune evasion.
format	Article
author	Khrustalev, Vladislav Victorovich Ghaznavi Rad, Ehsanollah Neela, Vasantha Kumari Shamsudin, Mariana Nor Amouzandeh-Nobaveh, Alireza Barkovsky, Eugene Victorovich
spellingShingle	Khrustalev, Vladislav Victorovich Ghaznavi Rad, Ehsanollah Neela, Vasantha Kumari Shamsudin, Mariana Nor Amouzandeh-Nobaveh, Alireza Barkovsky, Eugene Victorovich Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection
author_facet	Khrustalev, Vladislav Victorovich Ghaznavi Rad, Ehsanollah Neela, Vasantha Kumari Shamsudin, Mariana Nor Amouzandeh-Nobaveh, Alireza Barkovsky, Eugene Victorovich
author_sort	Khrustalev, Vladislav Victorovich
title	Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection
title_short	Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection
title_full	Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection
title_fullStr	Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection
title_full_unstemmed	Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection
title_sort	short repeats in the spa gene of staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection
publisher	Elsevier
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/29802/
_version_	1643829869235142656
score	13.211869

Short repeats in the spa gene of Staphylococcus aureus are prone to nonsense mutations: stop codons can be found in strains isolated from patients with generalized infection

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