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The combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells

Umbilical cord blood (UCB) is an attractive source of hematopoietic stem and progenitor cells (HSPCs) for transplantation. However, the low number of HSPCs from a single UCB donor limits the direct transplantation of UCB to patients. Because little is known about the effects of the physical microenv...

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Main Authors: Kumar, Suresh, Hsiao, Jui Hsiang, Ling, Qing Dong, Ida, Dulinska Molak, Chen, Guoping, Chang, Yung, Chang, Yu, Chen, Yung Hung, Chen, Da Chung, Hsu, Shih Tien, Higuchi, Akon
Format: Article
Language:English
Published: Elsevier 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29796/1/The%20combined%20influence%20of%20substrate%20elasticity%20and%20surface.pdf
http://psasir.upm.edu.my/id/eprint/29796/
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spelling my.upm.eprints.297962015-09-18T00:54:38Z http://psasir.upm.edu.my/id/eprint/29796/ The combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells Kumar, Suresh Hsiao, Jui Hsiang Ling, Qing Dong Ida, Dulinska Molak Chen, Guoping Chang, Yung Chang, Yu Chen, Yung Hung Chen, Da Chung Hsu, Shih Tien Higuchi, Akon Umbilical cord blood (UCB) is an attractive source of hematopoietic stem and progenitor cells (HSPCs) for transplantation. However, the low number of HSPCs from a single UCB donor limits the direct transplantation of UCB to patients. Because little is known about the effects of the physical microenvironment on HSPC expansion, we investigated the exvivo expansion of HSPCs cultured on biomaterials with different elasticities and grafted with different nanosegments. Polyvinylalcohol-co-itaconic acid (PVA-IA)-coated dishes with different stiffnesses ranging from a 3.7kPa to 30.4kPa storage modulus were used. Fibronectin or an oligopeptide (CS1, EILDVPST) was grafted onto the PVA-IA substrates. High exvivo fold expansion of HSPCs was observed in the PVA-IA dishes grafted with fibronectin or CS1, which displayed an intermediate stiffness ranging from 12.2kPa to 30.4kPa. The fold expansion was more than 1.4 times higher than that cultured in tissue culture polystyrene dishes (TCPS, 12GPa). Furthermore, HSPCs cultured in fibronectin or CS1-grafted PVA-IA-coated dishes with a stiffness of 12.2-30.4kPa generated more pluripotent colony-forming units (CFU-GM and CFU-GEMM) than those in TCPS dishes. This result indicates that both the physical and biological properties of biomaterials affect the exvivo expansion of HSPCs. Elsevier 2013-10 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29796/1/The%20combined%20influence%20of%20substrate%20elasticity%20and%20surface.pdf Kumar, Suresh and Hsiao, Jui Hsiang and Ling, Qing Dong and Ida, Dulinska Molak and Chen, Guoping and Chang, Yung and Chang, Yu and Chen, Yung Hung and Chen, Da Chung and Hsu, Shih Tien and Higuchi, Akon (2013) The combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells. Biomaterials, 34 (31). 7632 -7644. ISSN 0142-9612; ESSN: 1878-5905
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Umbilical cord blood (UCB) is an attractive source of hematopoietic stem and progenitor cells (HSPCs) for transplantation. However, the low number of HSPCs from a single UCB donor limits the direct transplantation of UCB to patients. Because little is known about the effects of the physical microenvironment on HSPC expansion, we investigated the exvivo expansion of HSPCs cultured on biomaterials with different elasticities and grafted with different nanosegments. Polyvinylalcohol-co-itaconic acid (PVA-IA)-coated dishes with different stiffnesses ranging from a 3.7kPa to 30.4kPa storage modulus were used. Fibronectin or an oligopeptide (CS1, EILDVPST) was grafted onto the PVA-IA substrates. High exvivo fold expansion of HSPCs was observed in the PVA-IA dishes grafted with fibronectin or CS1, which displayed an intermediate stiffness ranging from 12.2kPa to 30.4kPa. The fold expansion was more than 1.4 times higher than that cultured in tissue culture polystyrene dishes (TCPS, 12GPa). Furthermore, HSPCs cultured in fibronectin or CS1-grafted PVA-IA-coated dishes with a stiffness of 12.2-30.4kPa generated more pluripotent colony-forming units (CFU-GM and CFU-GEMM) than those in TCPS dishes. This result indicates that both the physical and biological properties of biomaterials affect the exvivo expansion of HSPCs.
format Article
author Kumar, Suresh
Hsiao, Jui Hsiang
Ling, Qing Dong
Ida, Dulinska Molak
Chen, Guoping
Chang, Yung
Chang, Yu
Chen, Yung Hung
Chen, Da Chung
Hsu, Shih Tien
Higuchi, Akon
spellingShingle Kumar, Suresh
Hsiao, Jui Hsiang
Ling, Qing Dong
Ida, Dulinska Molak
Chen, Guoping
Chang, Yung
Chang, Yu
Chen, Yung Hung
Chen, Da Chung
Hsu, Shih Tien
Higuchi, Akon
The combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells
author_facet Kumar, Suresh
Hsiao, Jui Hsiang
Ling, Qing Dong
Ida, Dulinska Molak
Chen, Guoping
Chang, Yung
Chang, Yu
Chen, Yung Hung
Chen, Da Chung
Hsu, Shih Tien
Higuchi, Akon
author_sort Kumar, Suresh
title The combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells
title_short The combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells
title_full The combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells
title_fullStr The combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells
title_full_unstemmed The combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells
title_sort combined influence of substrate elasticity and surface-grafted molecules on the exvivo expansion of hematopoietic stem and progenitor cells
publisher Elsevier
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29796/1/The%20combined%20influence%20of%20substrate%20elasticity%20and%20surface.pdf
http://psasir.upm.edu.my/id/eprint/29796/
_version_ 1643829867388600320
score 13.244413

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