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Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR

Rattus argentiventer (Rice Field Rat) is responsible for destruction of paddy. The mammal is known for its rapid reproductive potential which can be a target for biological control of this species. Phospholipase C-zeta (PLC ζ) is a specific enzyme found in sperm of mammals responsible for triggering...

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Main Authors: A. S., Fadzlina, A. M. N., Nasyriq, M. N., Noor Faezah, Lee, L. H., Cheah, Yoke Kqueen, Sukardi, Sabrina
Format: Article
Language:English
Published: International Organization Of Scientific Research (IOSR) 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29791/1/Detection%20of%20PLC.pdf
http://psasir.upm.edu.my/id/eprint/29791/
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spelling my.upm.eprints.297912015-09-02T07:43:18Z http://psasir.upm.edu.my/id/eprint/29791/ Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR A. S., Fadzlina A. M. N., Nasyriq M. N., Noor Faezah Lee, L. H. Cheah, Yoke Kqueen Sukardi, Sabrina Rattus argentiventer (Rice Field Rat) is responsible for destruction of paddy. The mammal is known for its rapid reproductive potential which can be a target for biological control of this species. Phospholipase C-zeta (PLC ζ) is a specific enzyme found in sperm of mammals responsible for triggering calcium oscillations leading to egg activation during fertilization. It facilitates the first step of egg activation causing egg division and subsequent development into an embryo. The method used in this study was to identify PLC gene fragments from the testis of Rattus argentiventer using two-step Reverse Transcriptase Polymerase Chain Reaction (PCR) and quantified with Real Time Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR). Following that, sequencing of PLC was performed. The identified sequence was then keyed in into the BLAST portal in NCBI (National Centre of Bioinformatics) for comparison with a standard PLC sequence from Rattus norvegicus for sequence alignment. The result showed that PLCζ was present in Rattus argentiventer and qRTPCR could quantify the amount of PLCζ available.As such this would give additional information of detection and quantification techniques for rapid identification and detection of PLCζ to be carried out in the approach of controlling the rapid growth of Rattus argentiventer population. International Organization Of Scientific Research (IOSR) 2013-11 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29791/1/Detection%20of%20PLC.pdf A. S., Fadzlina and A. M. N., Nasyriq and M. N., Noor Faezah and Lee, L. H. and Cheah, Yoke Kqueen and Sukardi, Sabrina (2013) Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR. IOSR Journal of Pharmacy and Biological Sciences, 8 (3). pp. 65-74. ISSN 2319-7676; ESSN: 2278-3008
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Rattus argentiventer (Rice Field Rat) is responsible for destruction of paddy. The mammal is known for its rapid reproductive potential which can be a target for biological control of this species. Phospholipase C-zeta (PLC ζ) is a specific enzyme found in sperm of mammals responsible for triggering calcium oscillations leading to egg activation during fertilization. It facilitates the first step of egg activation causing egg division and subsequent development into an embryo. The method used in this study was to identify PLC gene fragments from the testis of Rattus argentiventer using two-step Reverse Transcriptase Polymerase Chain Reaction (PCR) and quantified with Real Time Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR). Following that, sequencing of PLC was performed. The identified sequence was then keyed in into the BLAST portal in NCBI (National Centre of Bioinformatics) for comparison with a standard PLC sequence from Rattus norvegicus for sequence alignment. The result showed that PLCζ was present in Rattus argentiventer and qRTPCR could quantify the amount of PLCζ available.As such this would give additional information of detection and quantification techniques for rapid identification and detection of PLCζ to be carried out in the approach of controlling the rapid growth of Rattus argentiventer population.
format Article
author A. S., Fadzlina
A. M. N., Nasyriq
M. N., Noor Faezah
Lee, L. H.
Cheah, Yoke Kqueen
Sukardi, Sabrina
spellingShingle A. S., Fadzlina
A. M. N., Nasyriq
M. N., Noor Faezah
Lee, L. H.
Cheah, Yoke Kqueen
Sukardi, Sabrina
Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR
author_facet A. S., Fadzlina
A. M. N., Nasyriq
M. N., Noor Faezah
Lee, L. H.
Cheah, Yoke Kqueen
Sukardi, Sabrina
author_sort A. S., Fadzlina
title Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR
title_short Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR
title_full Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR
title_fullStr Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR
title_full_unstemmed Detection of PLC-ζ from testis of Rattus argentiventer(rice-field rat) using RT-PCR and qRT-PCR
title_sort detection of plc-ζ from testis of rattus argentiventer(rice-field rat) using rt-pcr and qrt-pcr
publisher International Organization Of Scientific Research (IOSR)
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29791/1/Detection%20of%20PLC.pdf
http://psasir.upm.edu.my/id/eprint/29791/
_version_ 1643829865905913856
score 13.211869

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