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Suppressions of serotonin-induced increased vascular permeability and leukocyte infiltration by Bixa orellana leaf extract

The aim of the present study was to evaluate the anti-inflammatory activities of aqueous extract of Bixa orellana (AEBO) leaves and its possible mechanisms in animal models. The anti-inflammatory activity of the extract was evaluated using serotonin-induced rat paw edema, increased peritoneal vascul...

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Main Authors: Yong, Yoke Keong, Sulaiman, Nur Shahira, Abdullah, Muhammad Nazrul Hakim, Ee, Gwendoline Cheng Lian, Zakaria, Zainul Amiruddin, Othman, Fauziah, Ahmad, Zuraini
Format: Article
Language:English
Published: Hindawi Publishing Corporation 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29608/1/Suppressions%20of%20serotonin.pdf
http://psasir.upm.edu.my/id/eprint/29608/
http://www.hindawi.com/journals/bmri/2013/463145/
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spelling my.upm.eprints.296082015-10-26T02:36:13Z http://psasir.upm.edu.my/id/eprint/29608/ Suppressions of serotonin-induced increased vascular permeability and leukocyte infiltration by Bixa orellana leaf extract Yong, Yoke Keong Sulaiman, Nur Shahira Abdullah, Muhammad Nazrul Hakim Ee, Gwendoline Cheng Lian Zakaria, Zainul Amiruddin Othman, Fauziah Ahmad, Zuraini The aim of the present study was to evaluate the anti-inflammatory activities of aqueous extract of Bixa orellana (AEBO) leaves and its possible mechanisms in animal models. The anti-inflammatory activity of the extract was evaluated using serotonin-induced rat paw edema, increased peritoneal vascular permeability, and leukocyte infiltrations in an air-pouch model. Nitric oxide (NO), indicated by the sum of nitrites and nitrates, and vascular growth endothelial growth factor (VEGF) were measured in paw tissues of rats to determine their involvement in the regulation of increased permeability. Pretreatments with AEBO (50 and 150 mg kg⁻¹) prior to serotonin inductions resulted in maximum inhibitions of 56.2% of paw volume, 45.7% of Evans blue dye leakage in the peritoneal vascular permeability model, and 83.9% of leukocyte infiltration in the air-pouch model. 57.2% maximum inhibition of NO and 27% of VEGF formations in rats' paws were observed with AEBO at the dose of 150 mg kg⁻¹. Pharmacological screening of the extract showed significant (P < 0.05) anti-inflammatory activity, indicated by the suppressions of increased vascular permeability and leukocyte infiltration. The inhibitions of these inflammatory events are probably mediated via inhibition of NO and VEGF formation and release. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29608/1/Suppressions%20of%20serotonin.pdf Yong, Yoke Keong and Sulaiman, Nur Shahira and Abdullah, Muhammad Nazrul Hakim and Ee, Gwendoline Cheng Lian and Zakaria, Zainul Amiruddin and Othman, Fauziah and Ahmad, Zuraini (2013) Suppressions of serotonin-induced increased vascular permeability and leukocyte infiltration by Bixa orellana leaf extract. BioMed Research International, 2013. art. no. 463145. pp. 1-7. ISSN 2314-6133; ESSN: 2314-6141 http://www.hindawi.com/journals/bmri/2013/463145/ 10.1155/2013/463145
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The aim of the present study was to evaluate the anti-inflammatory activities of aqueous extract of Bixa orellana (AEBO) leaves and its possible mechanisms in animal models. The anti-inflammatory activity of the extract was evaluated using serotonin-induced rat paw edema, increased peritoneal vascular permeability, and leukocyte infiltrations in an air-pouch model. Nitric oxide (NO), indicated by the sum of nitrites and nitrates, and vascular growth endothelial growth factor (VEGF) were measured in paw tissues of rats to determine their involvement in the regulation of increased permeability. Pretreatments with AEBO (50 and 150 mg kg⁻¹) prior to serotonin inductions resulted in maximum inhibitions of 56.2% of paw volume, 45.7% of Evans blue dye leakage in the peritoneal vascular permeability model, and 83.9% of leukocyte infiltration in the air-pouch model. 57.2% maximum inhibition of NO and 27% of VEGF formations in rats' paws were observed with AEBO at the dose of 150 mg kg⁻¹. Pharmacological screening of the extract showed significant (P < 0.05) anti-inflammatory activity, indicated by the suppressions of increased vascular permeability and leukocyte infiltration. The inhibitions of these inflammatory events are probably mediated via inhibition of NO and VEGF formation and release.
format Article
author Yong, Yoke Keong
Sulaiman, Nur Shahira
Abdullah, Muhammad Nazrul Hakim
Ee, Gwendoline Cheng Lian
Zakaria, Zainul Amiruddin
Othman, Fauziah
Ahmad, Zuraini
spellingShingle Yong, Yoke Keong
Sulaiman, Nur Shahira
Abdullah, Muhammad Nazrul Hakim
Ee, Gwendoline Cheng Lian
Zakaria, Zainul Amiruddin
Othman, Fauziah
Ahmad, Zuraini
Suppressions of serotonin-induced increased vascular permeability and leukocyte infiltration by Bixa orellana leaf extract
author_facet Yong, Yoke Keong
Sulaiman, Nur Shahira
Abdullah, Muhammad Nazrul Hakim
Ee, Gwendoline Cheng Lian
Zakaria, Zainul Amiruddin
Othman, Fauziah
Ahmad, Zuraini
author_sort Yong, Yoke Keong
title Suppressions of serotonin-induced increased vascular permeability and leukocyte infiltration by Bixa orellana leaf extract
title_short Suppressions of serotonin-induced increased vascular permeability and leukocyte infiltration by Bixa orellana leaf extract
title_full Suppressions of serotonin-induced increased vascular permeability and leukocyte infiltration by Bixa orellana leaf extract
title_fullStr Suppressions of serotonin-induced increased vascular permeability and leukocyte infiltration by Bixa orellana leaf extract
title_full_unstemmed Suppressions of serotonin-induced increased vascular permeability and leukocyte infiltration by Bixa orellana leaf extract
title_sort suppressions of serotonin-induced increased vascular permeability and leukocyte infiltration by bixa orellana leaf extract
publisher Hindawi Publishing Corporation
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29608/1/Suppressions%20of%20serotonin.pdf
http://psasir.upm.edu.my/id/eprint/29608/
http://www.hindawi.com/journals/bmri/2013/463145/
_version_ 1643829811658883072
score 13.211869

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