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Branched DNA: a novel technique for molecular diagnostics in bone studies

Recently, there are increasing applications of molecular techniques for molecular diagnostics in bone studies. Bone is a mineralized tissue containing an abundant matrix, which makes RNA isolation difficult. The most common technique used for molecular diagnosis in bone disease is reverse transcript...

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Main Author: Ahmad Ariffin, Siti Noor Fathilah
Format: Article
Language:English
Published: Faculty of Medicine, Universiti Kebangsaan Malaysia 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29552/1/Branched%20DNA%20a%20novel%20technique%20for%20molecular%20diagnostics%20in%20bone%20studies.pdf
http://psasir.upm.edu.my/id/eprint/29552/
http://spaj.ukm.my/fper/index.php/rumes/article/view/27
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spelling my.upm.eprints.295522016-07-12T08:38:00Z http://psasir.upm.edu.my/id/eprint/29552/ Branched DNA: a novel technique for molecular diagnostics in bone studies Ahmad Ariffin, Siti Noor Fathilah Recently, there are increasing applications of molecular techniques for molecular diagnostics in bone studies. Bone is a mineralized tissue containing an abundant matrix, which makes RNA isolation difficult. The most common technique used for molecular diagnosis in bone disease is reverse transcriptase–polymerase chain reaction (RT-PCR) sequence amplification assay. Currently, there is an in-creasing use of the branched chain DNA (bDNA) signal amplification technology. This article provides an overview of bDNA technology for molecular diagnostics in bone studies. Molecular diagnostic assays using bDNA technology for detection of gene expressions are sensitive, specific and reliable tools in the detection of genes involved in bone diseases such as osteoporosis. Faculty of Medicine, Universiti Kebangsaan Malaysia 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29552/1/Branched%20DNA%20a%20novel%20technique%20for%20molecular%20diagnostics%20in%20bone%20studies.pdf Ahmad Ariffin, Siti Noor Fathilah (2013) Branched DNA: a novel technique for molecular diagnostics in bone studies. Research Updates in Medical Sciences, 1 (1). pp. 27-29. ISSN 2289-2141 http://spaj.ukm.my/fper/index.php/rumes/article/view/27
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Recently, there are increasing applications of molecular techniques for molecular diagnostics in bone studies. Bone is a mineralized tissue containing an abundant matrix, which makes RNA isolation difficult. The most common technique used for molecular diagnosis in bone disease is reverse transcriptase–polymerase chain reaction (RT-PCR) sequence amplification assay. Currently, there is an in-creasing use of the branched chain DNA (bDNA) signal amplification technology. This article provides an overview of bDNA technology for molecular diagnostics in bone studies. Molecular diagnostic assays using bDNA technology for detection of gene expressions are sensitive, specific and reliable tools in the detection of genes involved in bone diseases such as osteoporosis.
format Article
author Ahmad Ariffin, Siti Noor Fathilah
spellingShingle Ahmad Ariffin, Siti Noor Fathilah
Branched DNA: a novel technique for molecular diagnostics in bone studies
author_facet Ahmad Ariffin, Siti Noor Fathilah
author_sort Ahmad Ariffin, Siti Noor Fathilah
title Branched DNA: a novel technique for molecular diagnostics in bone studies
title_short Branched DNA: a novel technique for molecular diagnostics in bone studies
title_full Branched DNA: a novel technique for molecular diagnostics in bone studies
title_fullStr Branched DNA: a novel technique for molecular diagnostics in bone studies
title_full_unstemmed Branched DNA: a novel technique for molecular diagnostics in bone studies
title_sort branched dna: a novel technique for molecular diagnostics in bone studies
publisher Faculty of Medicine, Universiti Kebangsaan Malaysia
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29552/1/Branched%20DNA%20a%20novel%20technique%20for%20molecular%20diagnostics%20in%20bone%20studies.pdf
http://psasir.upm.edu.my/id/eprint/29552/
http://spaj.ukm.my/fper/index.php/rumes/article/view/27
_version_ 1643829794535636992
score 13.211869

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