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Neuroprotective effects of biochanin A against glutamate-induced cytotoxicity in PC12 cells via apoptosis inhibition

L-Glutamate plays a crucial role in neuronal cell death, which is known to be associated with various neurodegenerative diseases, such as Alzheimer’s, Parkinson’s, and Huntington’s diseases. In this study, we investigated the protective effects of biochanin A, a phytoestrogen compound found mainly i...

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Main Authors: Tan, Ji Wei, Tham, Chau Ling, Israf Ali, Daud Ahmad, Lee, Sang Hyub, Kim, Min Kyu
Format: Article
Language:English
Published: Springer US 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29527/1/Neuroprotective%20effects%20of%20biochanin%20A%20against%20glutamate.pdf
http://psasir.upm.edu.my/id/eprint/29527/
http://link.springer.com/article/10.1007/s11064-012-0943-6
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spelling my.upm.eprints.295272015-12-29T02:51:18Z http://psasir.upm.edu.my/id/eprint/29527/ Neuroprotective effects of biochanin A against glutamate-induced cytotoxicity in PC12 cells via apoptosis inhibition Tan, Ji Wei Tham, Chau Ling Israf Ali, Daud Ahmad Lee, Sang Hyub Kim, Min Kyu L-Glutamate plays a crucial role in neuronal cell death, which is known to be associated with various neurodegenerative diseases, such as Alzheimer’s, Parkinson’s, and Huntington’s diseases. In this study, we investigated the protective effects of biochanin A, a phytoestrogen compound found mainly in Trifolium pratense, against L-glutamate-induced cytotoxicity in a PC12 cell line. Exposure of the cells to 10 mM l-glutamate was found to significantly increase cell viability loss and apoptosis, whereas pretreatment with various concentrations of biochanin A attenuated the cytotoxic effects of l-glutamate. Specifically, the pretreatment led to not only decreases in the release of lactate dehydrogenase, the number of apoptotic cells, and the activity of caspase-3 but also an increase in the total glutathione level in the L-glutamate-treated PC12 cells. These results indicate that biochanin A may be able to exert neuroprotective effects against L-glutamate-induced cytotoxicity. Furthermore, our findings also imply that biochanin A may act as an antiapoptotic agent in order to perform its protective function. Springer US 2013-03 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29527/1/Neuroprotective%20effects%20of%20biochanin%20A%20against%20glutamate.pdf Tan, Ji Wei and Tham, Chau Ling and Israf Ali, Daud Ahmad and Lee, Sang Hyub and Kim, Min Kyu (2013) Neuroprotective effects of biochanin A against glutamate-induced cytotoxicity in PC12 cells via apoptosis inhibition. Neurochemical Research, 38 (3). pp. 512-518. ISSN 0364-3190; ESSN: 1573-6903 http://link.springer.com/article/10.1007/s11064-012-0943-6 10.1007/s11064-012-0943-6
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description L-Glutamate plays a crucial role in neuronal cell death, which is known to be associated with various neurodegenerative diseases, such as Alzheimer’s, Parkinson’s, and Huntington’s diseases. In this study, we investigated the protective effects of biochanin A, a phytoestrogen compound found mainly in Trifolium pratense, against L-glutamate-induced cytotoxicity in a PC12 cell line. Exposure of the cells to 10 mM l-glutamate was found to significantly increase cell viability loss and apoptosis, whereas pretreatment with various concentrations of biochanin A attenuated the cytotoxic effects of l-glutamate. Specifically, the pretreatment led to not only decreases in the release of lactate dehydrogenase, the number of apoptotic cells, and the activity of caspase-3 but also an increase in the total glutathione level in the L-glutamate-treated PC12 cells. These results indicate that biochanin A may be able to exert neuroprotective effects against L-glutamate-induced cytotoxicity. Furthermore, our findings also imply that biochanin A may act as an antiapoptotic agent in order to perform its protective function.
format Article
author Tan, Ji Wei
Tham, Chau Ling
Israf Ali, Daud Ahmad
Lee, Sang Hyub
Kim, Min Kyu
spellingShingle Tan, Ji Wei
Tham, Chau Ling
Israf Ali, Daud Ahmad
Lee, Sang Hyub
Kim, Min Kyu
Neuroprotective effects of biochanin A against glutamate-induced cytotoxicity in PC12 cells via apoptosis inhibition
author_facet Tan, Ji Wei
Tham, Chau Ling
Israf Ali, Daud Ahmad
Lee, Sang Hyub
Kim, Min Kyu
author_sort Tan, Ji Wei
title Neuroprotective effects of biochanin A against glutamate-induced cytotoxicity in PC12 cells via apoptosis inhibition
title_short Neuroprotective effects of biochanin A against glutamate-induced cytotoxicity in PC12 cells via apoptosis inhibition
title_full Neuroprotective effects of biochanin A against glutamate-induced cytotoxicity in PC12 cells via apoptosis inhibition
title_fullStr Neuroprotective effects of biochanin A against glutamate-induced cytotoxicity in PC12 cells via apoptosis inhibition
title_full_unstemmed Neuroprotective effects of biochanin A against glutamate-induced cytotoxicity in PC12 cells via apoptosis inhibition
title_sort neuroprotective effects of biochanin a against glutamate-induced cytotoxicity in pc12 cells via apoptosis inhibition
publisher Springer US
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29527/1/Neuroprotective%20effects%20of%20biochanin%20A%20against%20glutamate.pdf
http://psasir.upm.edu.my/id/eprint/29527/
http://link.springer.com/article/10.1007/s11064-012-0943-6
_version_ 1643829786816020480
score 13.211869

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