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A short-term n-3 DPA supplementation study in humans

Purpose: Despite the detailed knowledge of the absorption and incorporation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) into plasma lipids and red blood cells (RBC) in humans, very little is known about docosapentaenoic acid (DPA, 22:5 n-3). The aim of this study was to investigate...

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Main Authors: Miller, Eliza, Kaur, Gunveen, Larsen, Amy, Loh, Su Peng, Linderborg, Kaisa, Weisinger, Harrison S., Turchini, Giovanni M., Cameron-Smith, David, Sinclair, Andrew J.
Format: Article
Language:English
Published: Springer 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29525/1/A%20short-term%20n-3%20DPA%20supplementation%20study%20in%20humans.pdf
http://psasir.upm.edu.my/id/eprint/29525/
http://link.springer.com/article/10.1007/s00394-012-0396-3?view=classic
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spelling my.upm.eprints.295252016-07-12T09:23:44Z http://psasir.upm.edu.my/id/eprint/29525/ A short-term n-3 DPA supplementation study in humans Miller, Eliza Kaur, Gunveen Larsen, Amy Loh, Su Peng Linderborg, Kaisa Weisinger, Harrison S. Turchini, Giovanni M. Cameron-Smith, David Sinclair, Andrew J. Purpose: Despite the detailed knowledge of the absorption and incorporation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) into plasma lipids and red blood cells (RBC) in humans, very little is known about docosapentaenoic acid (DPA, 22:5 n-3). The aim of this study was to investigate the uptake and incorporation of pure DPA and EPA into human plasma and RBC lipids. Methods: Ten female participants received 8 g of pure DPA or pure EPA in randomized crossover double-blinded manner over a 7-day period. The placebo treatment was olive oil. Blood samples were collected at days zero, four and seven, following which the plasma and RBC were separated and used for the analysis of fatty acids. Results: Supplementation with DPA significantly increased the proportions of DPA in the plasma phospholipids (PL) (by twofold) and triacylglycerol (TAG) fractions (by 2.3-fold, day 4). DPA supplementation also significantly increased the proportions of EPA in TAG (by 3.1-fold, day 4) and cholesterol ester (CE) fractions (by 2.0-fold, day 7) and of DHA in TAG fraction (by 3.1-fold, day 4). DPA proportions in RBC PL did not change following supplementation. Supplementation with EPA significantly increased the proportion of EPA in the plasma CE and PL fractions, (both by 2.7-fold, day 4 and day 7) and in the RBC PL (by 1.9-fold, day 4 and day 7). EPA supplementation did not alter the proportions of DPA or DHA in any lipid fraction. These results showed that within day 4 of supplementation, DPA and EPA demonstrated different and specific incorporation patterns. Conclusion: The results of this short-term study suggest that DPA may act as a reservoir of the major long-chain n-3 fatty acids (LC n-3 PUFA) in humans. Springer 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29525/1/A%20short-term%20n-3%20DPA%20supplementation%20study%20in%20humans.pdf Miller, Eliza and Kaur, Gunveen and Larsen, Amy and Loh, Su Peng and Linderborg, Kaisa and Weisinger, Harrison S. and Turchini, Giovanni M. and Cameron-Smith, David and Sinclair, Andrew J. (2013) A short-term n-3 DPA supplementation study in humans. European Journal of Nutrition, 52 (3). pp. 895-904. ISSN 1436-6207; ESSN: 1436-6215 http://link.springer.com/article/10.1007/s00394-012-0396-3?view=classic 10.1007/s00394-012-0396-3
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Purpose: Despite the detailed knowledge of the absorption and incorporation of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) into plasma lipids and red blood cells (RBC) in humans, very little is known about docosapentaenoic acid (DPA, 22:5 n-3). The aim of this study was to investigate the uptake and incorporation of pure DPA and EPA into human plasma and RBC lipids. Methods: Ten female participants received 8 g of pure DPA or pure EPA in randomized crossover double-blinded manner over a 7-day period. The placebo treatment was olive oil. Blood samples were collected at days zero, four and seven, following which the plasma and RBC were separated and used for the analysis of fatty acids. Results: Supplementation with DPA significantly increased the proportions of DPA in the plasma phospholipids (PL) (by twofold) and triacylglycerol (TAG) fractions (by 2.3-fold, day 4). DPA supplementation also significantly increased the proportions of EPA in TAG (by 3.1-fold, day 4) and cholesterol ester (CE) fractions (by 2.0-fold, day 7) and of DHA in TAG fraction (by 3.1-fold, day 4). DPA proportions in RBC PL did not change following supplementation. Supplementation with EPA significantly increased the proportion of EPA in the plasma CE and PL fractions, (both by 2.7-fold, day 4 and day 7) and in the RBC PL (by 1.9-fold, day 4 and day 7). EPA supplementation did not alter the proportions of DPA or DHA in any lipid fraction. These results showed that within day 4 of supplementation, DPA and EPA demonstrated different and specific incorporation patterns. Conclusion: The results of this short-term study suggest that DPA may act as a reservoir of the major long-chain n-3 fatty acids (LC n-3 PUFA) in humans.
format Article
author Miller, Eliza
Kaur, Gunveen
Larsen, Amy
Loh, Su Peng
Linderborg, Kaisa
Weisinger, Harrison S.
Turchini, Giovanni M.
Cameron-Smith, David
Sinclair, Andrew J.
spellingShingle Miller, Eliza
Kaur, Gunveen
Larsen, Amy
Loh, Su Peng
Linderborg, Kaisa
Weisinger, Harrison S.
Turchini, Giovanni M.
Cameron-Smith, David
Sinclair, Andrew J.
A short-term n-3 DPA supplementation study in humans
author_facet Miller, Eliza
Kaur, Gunveen
Larsen, Amy
Loh, Su Peng
Linderborg, Kaisa
Weisinger, Harrison S.
Turchini, Giovanni M.
Cameron-Smith, David
Sinclair, Andrew J.
author_sort Miller, Eliza
title A short-term n-3 DPA supplementation study in humans
title_short A short-term n-3 DPA supplementation study in humans
title_full A short-term n-3 DPA supplementation study in humans
title_fullStr A short-term n-3 DPA supplementation study in humans
title_full_unstemmed A short-term n-3 DPA supplementation study in humans
title_sort short-term n-3 dpa supplementation study in humans
publisher Springer
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29525/1/A%20short-term%20n-3%20DPA%20supplementation%20study%20in%20humans.pdf
http://psasir.upm.edu.my/id/eprint/29525/
http://link.springer.com/article/10.1007/s00394-012-0396-3?view=classic
_version_ 1643829786220429312
score 13.211869

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