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Morphological and molecular characterization of phytophthora capsici, the causal agent of foot rot disease of black pepper in Sarawak, Malaysia

Sarawak is one of the largest exporters of black pepper (Piper nigrum L.) but the production of this crop is in the decline, because of the foot rot disease. The objective of this study was to determine the morphological and molecular characters of the Phytophthora capsici the causal agent of foot r...

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Main Authors: Md Daut, Siti Noor Farhana, M. Shahul Hameed, Rahamath Bivi, Ahmad, Khairulmazmi, Wong, Sing King, Meon, Sariah
Format: Article
Language:English
Published: Friends Science Publishers 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29372/1/Morphological%20and%20molecular%20characterization%20of%20phytophthora%20capsici%2C%20the%20causal%20agent%20of%20foot%20rot%20disease%20of%20black%20pepper%20in%20Sarawak%2C%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/29372/
http://www.fspublishers.org/Issue.php?categoryID=118
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spelling my.upm.eprints.293722016-07-13T01:48:29Z http://psasir.upm.edu.my/id/eprint/29372/ Morphological and molecular characterization of phytophthora capsici, the causal agent of foot rot disease of black pepper in Sarawak, Malaysia Md Daut, Siti Noor Farhana M. Shahul Hameed, Rahamath Bivi Ahmad, Khairulmazmi Wong, Sing King Meon, Sariah Sarawak is one of the largest exporters of black pepper (Piper nigrum L.) but the production of this crop is in the decline, because of the foot rot disease. The objective of this study was to determine the morphological and molecular characters of the Phytophthora capsici the causal agent of foot rot disease of black pepper in Sarawak. Thirteen major pepper growing areas were surveyed and confirmed for the incidence of foot rot disease. Ulu Sarikei (Sarikei) had the highest disease incidence (75%) followed by Pasai Siong (Sibu) (70%) and the lowest incidence at Tatau (Bintulu) (5%). The highest disease severity was at Ulu Sarikei (70%) followed by Pasai Siong (62%) and the lowest at Tatau (4%). Based on morphological characterization, the foot rot pathogen exhibited globose oogonia with paragynous antheridia, chlamydospore, torulose hyphae and lemon shaped sporangia with long pedicel. Molecular identification by using nested-PCR showed unique DNA fragment of c. 560 bp further confirmed that the causal agent of foot rot disease of black pepper in Sarawak was P. capsici Leonian. Friends Science Publishers 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29372/1/Morphological%20and%20molecular%20characterization%20of%20phytophthora%20capsici%2C%20the%20causal%20agent%20of%20foot%20rot%20disease%20of%20black%20pepper%20in%20Sarawak%2C%20Malaysia.pdf Md Daut, Siti Noor Farhana and M. Shahul Hameed, Rahamath Bivi and Ahmad, Khairulmazmi and Wong, Sing King and Meon, Sariah (2013) Morphological and molecular characterization of phytophthora capsici, the causal agent of foot rot disease of black pepper in Sarawak, Malaysia. International Journal of Agriculture and Biology, 15 (6). pp. 1083-1090. ISSN 1560-8530; ESSN: 1814-9596 http://www.fspublishers.org/Issue.php?categoryID=118
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Sarawak is one of the largest exporters of black pepper (Piper nigrum L.) but the production of this crop is in the decline, because of the foot rot disease. The objective of this study was to determine the morphological and molecular characters of the Phytophthora capsici the causal agent of foot rot disease of black pepper in Sarawak. Thirteen major pepper growing areas were surveyed and confirmed for the incidence of foot rot disease. Ulu Sarikei (Sarikei) had the highest disease incidence (75%) followed by Pasai Siong (Sibu) (70%) and the lowest incidence at Tatau (Bintulu) (5%). The highest disease severity was at Ulu Sarikei (70%) followed by Pasai Siong (62%) and the lowest at Tatau (4%). Based on morphological characterization, the foot rot pathogen exhibited globose oogonia with paragynous antheridia, chlamydospore, torulose hyphae and lemon shaped sporangia with long pedicel. Molecular identification by using nested-PCR showed unique DNA fragment of c. 560 bp further confirmed that the causal agent of foot rot disease of black pepper in Sarawak was P. capsici Leonian.
format Article
author Md Daut, Siti Noor Farhana
M. Shahul Hameed, Rahamath Bivi
Ahmad, Khairulmazmi
Wong, Sing King
Meon, Sariah
spellingShingle Md Daut, Siti Noor Farhana
M. Shahul Hameed, Rahamath Bivi
Ahmad, Khairulmazmi
Wong, Sing King
Meon, Sariah
Morphological and molecular characterization of phytophthora capsici, the causal agent of foot rot disease of black pepper in Sarawak, Malaysia
author_facet Md Daut, Siti Noor Farhana
M. Shahul Hameed, Rahamath Bivi
Ahmad, Khairulmazmi
Wong, Sing King
Meon, Sariah
author_sort Md Daut, Siti Noor Farhana
title Morphological and molecular characterization of phytophthora capsici, the causal agent of foot rot disease of black pepper in Sarawak, Malaysia
title_short Morphological and molecular characterization of phytophthora capsici, the causal agent of foot rot disease of black pepper in Sarawak, Malaysia
title_full Morphological and molecular characterization of phytophthora capsici, the causal agent of foot rot disease of black pepper in Sarawak, Malaysia
title_fullStr Morphological and molecular characterization of phytophthora capsici, the causal agent of foot rot disease of black pepper in Sarawak, Malaysia
title_full_unstemmed Morphological and molecular characterization of phytophthora capsici, the causal agent of foot rot disease of black pepper in Sarawak, Malaysia
title_sort morphological and molecular characterization of phytophthora capsici, the causal agent of foot rot disease of black pepper in sarawak, malaysia
publisher Friends Science Publishers
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29372/1/Morphological%20and%20molecular%20characterization%20of%20phytophthora%20capsici%2C%20the%20causal%20agent%20of%20foot%20rot%20disease%20of%20black%20pepper%20in%20Sarawak%2C%20Malaysia.pdf
http://psasir.upm.edu.my/id/eprint/29372/
http://www.fspublishers.org/Issue.php?categoryID=118
_version_ 1643829741216595968
score 13.211869

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