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The effects of polymorphisms in 7 candidate genes on resistance to Salmonella Enteritidis in native chickens

Salmonella enterica serovar Enteritidis infection is a common concern in poultry production for its negative effects on growth as well as food safety for humans. Identification of molecular markers that are linked to resistance to Salmonella Enteritidis may lead to appropriate solutions to control S...

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Main Authors: Tohidi, Reza, Idris, Ismail, Panandam, Jothi Malar, Bejo, Mohd Hair
Format: Article
Language:English
Published: Oxford University Press 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29327/1/The%20effects%20of%20polymorphisms%20in%207%20candidate%20genes%20on%20resistance%20to%20Salmonella%20Enteritidis%20in%20native%20chickens.pdf
http://psasir.upm.edu.my/id/eprint/29327/
http://ps.oxfordjournals.org/content/92/4/900.abstract
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spelling my.upm.eprints.293272016-07-13T04:36:53Z http://psasir.upm.edu.my/id/eprint/29327/ The effects of polymorphisms in 7 candidate genes on resistance to Salmonella Enteritidis in native chickens Tohidi, Reza Idris, Ismail Panandam, Jothi Malar Bejo, Mohd Hair Salmonella enterica serovar Enteritidis infection is a common concern in poultry production for its negative effects on growth as well as food safety for humans. Identification of molecular markers that are linked to resistance to Salmonella Enteritidis may lead to appropriate solutions to control Salmonella infection in chickens. This study investigated the association of candidate genes with resistance to Salmonella Enteritidis in young chickens. Two native breeds of Malaysian chickens, namely, Village Chickens and Red Junglefowl, were evaluated for bacterial colonization after Salmonella Enteritidis inoculation. Seven candidate genes were selected on the basis of their physiological role in immune response, as determined by prior studies in other genetic lines: natural resistance-associated protein 1 (NRAMP1), transforming growth factor β3 (TGFβ3), transforming growth factor β4 (TGFβ4), inhibitor of apoptosis protein 1 (IAP1), caspase 1 (CASP1), lipopolysaccharide-induced tumor necrosis factor (TNF) α factor (LITAF), and TNF-related apoptosis-inducing ligand (TRAIL). Polymerase chain reaction-RFLP was used to identify polymorphisms in the candidate genes; all genes exhibited polymorphisms in at least one breed. The NRAMP1-SacI polymorphism correlated with the differences in Salmonella Enteritidis load in the cecum (P = 0.002) and spleen (P = 0.01) of Village Chickens. Polymorphisms in the restriction sites of TGFβ3-BsrI, TGFβ4-MboII, and TRAIL-StyI were associated with Salmonella Enteritidis burden in the cecum, spleen, and liver of Village Chickens and Red Junglefowl (P < 0.05). These results indicate that the NRAMP1, TGFβ3, TGFβ4, and TRAIL genes are potential candidates for use in selection programs for increasing genetic resistance against Salmonella Enteritidis in native Malaysian chickens. Oxford University Press 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29327/1/The%20effects%20of%20polymorphisms%20in%207%20candidate%20genes%20on%20resistance%20to%20Salmonella%20Enteritidis%20in%20native%20chickens.pdf Tohidi, Reza and Idris, Ismail and Panandam, Jothi Malar and Bejo, Mohd Hair (2013) The effects of polymorphisms in 7 candidate genes on resistance to Salmonella Enteritidis in native chickens. Poultry Science, 92 (4). pp. 900-909. ISSN 0032-5791; ESSN: 1525-3171 http://ps.oxfordjournals.org/content/92/4/900.abstract 10.3382/ps.2012-02797
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Salmonella enterica serovar Enteritidis infection is a common concern in poultry production for its negative effects on growth as well as food safety for humans. Identification of molecular markers that are linked to resistance to Salmonella Enteritidis may lead to appropriate solutions to control Salmonella infection in chickens. This study investigated the association of candidate genes with resistance to Salmonella Enteritidis in young chickens. Two native breeds of Malaysian chickens, namely, Village Chickens and Red Junglefowl, were evaluated for bacterial colonization after Salmonella Enteritidis inoculation. Seven candidate genes were selected on the basis of their physiological role in immune response, as determined by prior studies in other genetic lines: natural resistance-associated protein 1 (NRAMP1), transforming growth factor β3 (TGFβ3), transforming growth factor β4 (TGFβ4), inhibitor of apoptosis protein 1 (IAP1), caspase 1 (CASP1), lipopolysaccharide-induced tumor necrosis factor (TNF) α factor (LITAF), and TNF-related apoptosis-inducing ligand (TRAIL). Polymerase chain reaction-RFLP was used to identify polymorphisms in the candidate genes; all genes exhibited polymorphisms in at least one breed. The NRAMP1-SacI polymorphism correlated with the differences in Salmonella Enteritidis load in the cecum (P = 0.002) and spleen (P = 0.01) of Village Chickens. Polymorphisms in the restriction sites of TGFβ3-BsrI, TGFβ4-MboII, and TRAIL-StyI were associated with Salmonella Enteritidis burden in the cecum, spleen, and liver of Village Chickens and Red Junglefowl (P < 0.05). These results indicate that the NRAMP1, TGFβ3, TGFβ4, and TRAIL genes are potential candidates for use in selection programs for increasing genetic resistance against Salmonella Enteritidis in native Malaysian chickens.
format Article
author Tohidi, Reza
Idris, Ismail
Panandam, Jothi Malar
Bejo, Mohd Hair
spellingShingle Tohidi, Reza
Idris, Ismail
Panandam, Jothi Malar
Bejo, Mohd Hair
The effects of polymorphisms in 7 candidate genes on resistance to Salmonella Enteritidis in native chickens
author_facet Tohidi, Reza
Idris, Ismail
Panandam, Jothi Malar
Bejo, Mohd Hair
author_sort Tohidi, Reza
title The effects of polymorphisms in 7 candidate genes on resistance to Salmonella Enteritidis in native chickens
title_short The effects of polymorphisms in 7 candidate genes on resistance to Salmonella Enteritidis in native chickens
title_full The effects of polymorphisms in 7 candidate genes on resistance to Salmonella Enteritidis in native chickens
title_fullStr The effects of polymorphisms in 7 candidate genes on resistance to Salmonella Enteritidis in native chickens
title_full_unstemmed The effects of polymorphisms in 7 candidate genes on resistance to Salmonella Enteritidis in native chickens
title_sort effects of polymorphisms in 7 candidate genes on resistance to salmonella enteritidis in native chickens
publisher Oxford University Press
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29327/1/The%20effects%20of%20polymorphisms%20in%207%20candidate%20genes%20on%20resistance%20to%20Salmonella%20Enteritidis%20in%20native%20chickens.pdf
http://psasir.upm.edu.my/id/eprint/29327/
http://ps.oxfordjournals.org/content/92/4/900.abstract
_version_ 1643829727411044352
score 13.211869

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