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Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers

Background: Broccoli, Brassica oleracea subsp. italica is one of the many valuable Brassica species which is still less cultured under in vitro condition. Heat tolerant transgenic and non-transgenic broccoli cv. Green Marvel plantlets with well-developed root system obtained through in vitro culture...

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Main Authors: Ravanfar, Seyed Ali, Abdul Aziz, Maheran, Shabanimofrad, Mahmoodreza, Samarfard, Samira
Format: Article
Language:English
Published: Pontificia Universidad Catolica de Valparaiso 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29294/1/29294.pdf
http://psasir.upm.edu.my/id/eprint/29294/
http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/1362
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spelling my.upm.eprints.292942016-04-25T04:19:48Z http://psasir.upm.edu.my/id/eprint/29294/ Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers Ravanfar, Seyed Ali Abdul Aziz, Maheran Shabanimofrad, Mahmoodreza Samarfard, Samira Background: Broccoli, Brassica oleracea subsp. italica is one of the many valuable Brassica species which is still less cultured under in vitro condition. Heat tolerant transgenic and non-transgenic broccoli cv. Green Marvel plantlets with well-developed root system obtained through in vitro culture were transferred into disposable plastic pots containing sterilized potting mixture consisting of (peatgro™) + coconut dust (2:1) and maintained in a growth chamber. Results: After one month, the hardened plantlets were transferred and maintained in a transgenic greenhouse. After four months of acclimatization in the transgenic greenhouse, the efficacy of HSP101 gene in increasing the heat tolerance of the transgenic broccoli was evaluated. Results showed that the transgenic plants could survive and performed normally, producing flower heads even at the highest tested temperature of 34°C. Seven transgenic broccoli lines with different gene copy number of the AtHSP101 gene as well as the control plant were assessed for genetic diversity using inter simple sequence repeat (ISSR) markers. Conclusions: ISSR results showed polymorphism and phylogenetic relationship between the transgenic and non-transgenic (control) Brassica oleracea cv. Green Marvel. Pontificia Universidad Catolica de Valparaiso 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29294/1/29294.pdf Ravanfar, Seyed Ali and Abdul Aziz, Maheran and Shabanimofrad, Mahmoodreza and Samarfard, Samira (2013) Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers. Electronic Journal of Biotechnology, 16 (5). pp. 1-10. ISSN 0717-3458 http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/1362 10.2225/vol16-issue5-fulltext-10
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Broccoli, Brassica oleracea subsp. italica is one of the many valuable Brassica species which is still less cultured under in vitro condition. Heat tolerant transgenic and non-transgenic broccoli cv. Green Marvel plantlets with well-developed root system obtained through in vitro culture were transferred into disposable plastic pots containing sterilized potting mixture consisting of (peatgro™) + coconut dust (2:1) and maintained in a growth chamber. Results: After one month, the hardened plantlets were transferred and maintained in a transgenic greenhouse. After four months of acclimatization in the transgenic greenhouse, the efficacy of HSP101 gene in increasing the heat tolerance of the transgenic broccoli was evaluated. Results showed that the transgenic plants could survive and performed normally, producing flower heads even at the highest tested temperature of 34°C. Seven transgenic broccoli lines with different gene copy number of the AtHSP101 gene as well as the control plant were assessed for genetic diversity using inter simple sequence repeat (ISSR) markers. Conclusions: ISSR results showed polymorphism and phylogenetic relationship between the transgenic and non-transgenic (control) Brassica oleracea cv. Green Marvel.
format Article
author Ravanfar, Seyed Ali
Abdul Aziz, Maheran
Shabanimofrad, Mahmoodreza
Samarfard, Samira
spellingShingle Ravanfar, Seyed Ali
Abdul Aziz, Maheran
Shabanimofrad, Mahmoodreza
Samarfard, Samira
Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
author_facet Ravanfar, Seyed Ali
Abdul Aziz, Maheran
Shabanimofrad, Mahmoodreza
Samarfard, Samira
author_sort Ravanfar, Seyed Ali
title Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
title_short Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
title_full Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
title_fullStr Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
title_full_unstemmed Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers
title_sort greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (issr) markers
publisher Pontificia Universidad Catolica de Valparaiso
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29294/1/29294.pdf
http://psasir.upm.edu.my/id/eprint/29294/
http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/1362
_version_ 1643829717550235648
score 13.211869

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