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Development of hexaploid Brassica (AABBCC) from hybrids (ABC) of Brassica carinata (BBCC) × B. rapa (AA)

The aim of this research was to develop Brassica hexaploids from triploid hybrids using colchicine and to study the performances of the developed hexaploids. Triploid hybrids (genome, ABC) developed from crosses between Brassica carinata (genome, BBCC) and B. rapa (genome, AA) were used as source ma...

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Main Authors: Malek, Mohammad Abdul, Rahman, L., Das, M. L., Hassan, L., Yusop, Mohd Rafii, Ismail, Mohd Razi
Format: Article
Language:English
Published: Southern Cross Publishing 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29277/1/Development%20of%20hexaploid%20Brassica%20%28AABBCC%29%20from%20hybrids%20%28ABC%29%20of%20Brassica%20carinata%20%28BBCC%29%20%C3%97%20B.%20rapa%20%28AA%29.pdf
http://psasir.upm.edu.my/id/eprint/29277/
http://www.cropj.com/august2013.html
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spelling my.upm.eprints.292772016-06-20T08:09:35Z http://psasir.upm.edu.my/id/eprint/29277/ Development of hexaploid Brassica (AABBCC) from hybrids (ABC) of Brassica carinata (BBCC) × B. rapa (AA) Malek, Mohammad Abdul Rahman, L. Das, M. L. Hassan, L. Yusop, Mohd Rafii Ismail, Mohd Razi The aim of this research was to develop Brassica hexaploids from triploid hybrids using colchicine and to study the performances of the developed hexaploids. Triploid hybrids (genome, ABC) developed from crosses between Brassica carinata (genome, BBCC) and B. rapa (genome, AA) were used as source materials for chromosome doubling through colchicine treatment to develop Brassica hexaploids (genome, AABBCC). Modified injection method was found to be more effective than cotton plug method for doubling the chromosome numbers. On an average, the use of 0.10, 0.15 and 0.20% colchicine gave 36.4, 56.0 and 71.8% success in chromosome doubling by modified injection method compared to 27.4, 38.0 and 39.1% success by cotton plug method. First generation (H1) hexaploids produced larger buds and flowers than those of both parents and triploid hybrids. The untreated triploid hybrids did not produce seeds due to having sterile pollens only while hexaploids produced fertile pollens and seeds. On an average, 59% of second generation (H2) hexaploid seeds were euploid, where the rest of 41% seeds were aneuploid. Among the H2 hexaploid lines, four were selected as they produced significantly higher seed yield/plant (11.57-12.4 g) compared to their parental genotypes (8.13-9.93 g). These four H2 hexaploid lines can be used for family line development in the subsequent generations to select stable and desirable Brassica hexaploids. Southern Cross Publishing 2013-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29277/1/Development%20of%20hexaploid%20Brassica%20%28AABBCC%29%20from%20hybrids%20%28ABC%29%20of%20Brassica%20carinata%20%28BBCC%29%20%C3%97%20B.%20rapa%20%28AA%29.pdf Malek, Mohammad Abdul and Rahman, L. and Das, M. L. and Hassan, L. and Yusop, Mohd Rafii and Ismail, Mohd Razi (2013) Development of hexaploid Brassica (AABBCC) from hybrids (ABC) of Brassica carinata (BBCC) × B. rapa (AA). Australian Journal of Crop Science, 7 (9). pp. 1375-1382. ISSN 1835-2693; ESSN: 1835-2707 http://www.cropj.com/august2013.html
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The aim of this research was to develop Brassica hexaploids from triploid hybrids using colchicine and to study the performances of the developed hexaploids. Triploid hybrids (genome, ABC) developed from crosses between Brassica carinata (genome, BBCC) and B. rapa (genome, AA) were used as source materials for chromosome doubling through colchicine treatment to develop Brassica hexaploids (genome, AABBCC). Modified injection method was found to be more effective than cotton plug method for doubling the chromosome numbers. On an average, the use of 0.10, 0.15 and 0.20% colchicine gave 36.4, 56.0 and 71.8% success in chromosome doubling by modified injection method compared to 27.4, 38.0 and 39.1% success by cotton plug method. First generation (H1) hexaploids produced larger buds and flowers than those of both parents and triploid hybrids. The untreated triploid hybrids did not produce seeds due to having sterile pollens only while hexaploids produced fertile pollens and seeds. On an average, 59% of second generation (H2) hexaploid seeds were euploid, where the rest of 41% seeds were aneuploid. Among the H2 hexaploid lines, four were selected as they produced significantly higher seed yield/plant (11.57-12.4 g) compared to their parental genotypes (8.13-9.93 g). These four H2 hexaploid lines can be used for family line development in the subsequent generations to select stable and desirable Brassica hexaploids.
format Article
author Malek, Mohammad Abdul
Rahman, L.
Das, M. L.
Hassan, L.
Yusop, Mohd Rafii
Ismail, Mohd Razi
spellingShingle Malek, Mohammad Abdul
Rahman, L.
Das, M. L.
Hassan, L.
Yusop, Mohd Rafii
Ismail, Mohd Razi
Development of hexaploid Brassica (AABBCC) from hybrids (ABC) of Brassica carinata (BBCC) × B. rapa (AA)
author_facet Malek, Mohammad Abdul
Rahman, L.
Das, M. L.
Hassan, L.
Yusop, Mohd Rafii
Ismail, Mohd Razi
author_sort Malek, Mohammad Abdul
title Development of hexaploid Brassica (AABBCC) from hybrids (ABC) of Brassica carinata (BBCC) × B. rapa (AA)
title_short Development of hexaploid Brassica (AABBCC) from hybrids (ABC) of Brassica carinata (BBCC) × B. rapa (AA)
title_full Development of hexaploid Brassica (AABBCC) from hybrids (ABC) of Brassica carinata (BBCC) × B. rapa (AA)
title_fullStr Development of hexaploid Brassica (AABBCC) from hybrids (ABC) of Brassica carinata (BBCC) × B. rapa (AA)
title_full_unstemmed Development of hexaploid Brassica (AABBCC) from hybrids (ABC) of Brassica carinata (BBCC) × B. rapa (AA)
title_sort development of hexaploid brassica (aabbcc) from hybrids (abc) of brassica carinata (bbcc) × b. rapa (aa)
publisher Southern Cross Publishing
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29277/1/Development%20of%20hexaploid%20Brassica%20%28AABBCC%29%20from%20hybrids%20%28ABC%29%20of%20Brassica%20carinata%20%28BBCC%29%20%C3%97%20B.%20rapa%20%28AA%29.pdf
http://psasir.upm.edu.my/id/eprint/29277/
http://www.cropj.com/august2013.html
_version_ 1643829712776069120
score 13.211869

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