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Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like bodies as affected by chitosan

Chitosan is a carbohydrate polymer derivative of chitin which presents in shell of crustaceans. This biopolymer is a non toxic and environmentally friendly, considered as a plant growth stimulator in some plant species. The present study investigates the effects of chitosan and media types on multip...

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Main Authors: Samarfard, Samira, Abdul Kadir, Mihdzar, Kadzimin, Saleh, Ravanfar, Seyedali, Mohd Saud, Halimi
Format: Article
Language:English
Published: AcademicPres 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29275/1/29275.pdf
http://psasir.upm.edu.my/id/eprint/29275/
http://www.notulaebotanicae.ro/index.php/nbha/article/view/8315
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spelling my.upm.eprints.292752016-04-25T03:11:59Z http://psasir.upm.edu.my/id/eprint/29275/ Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like bodies as affected by chitosan Samarfard, Samira Abdul Kadir, Mihdzar Kadzimin, Saleh Ravanfar, Seyedali Mohd Saud, Halimi Chitosan is a carbohydrate polymer derivative of chitin which presents in shell of crustaceans. This biopolymer is a non toxic and environmentally friendly, considered as a plant growth stimulator in some plant species. The present study investigates the effects of chitosan and media types on multiplication and genetic stability of Phalaenopsis gigantea protocorm-like bodies (PLBs). PLBs were inoculated in liquid New Dogashima Medium (NDM) and Vacin and Went (VW) supplemented with various concentrations of chitosan (0, 5, 10, 15, 20 and 25 mg/L). The highest PLB multiplication was observed on VW and NDM supplemented with 10 mg/L chitosan with mean number of PLBs 177 and 147, respectively. Chitosan promoted the formation of juvenile leaves and the highest number was observed in NDM supplemented with 20 mg/L chitosan with mean number of 66 leaves after 8 weeks of culture. Genetic stability was assessed among mother plant and secondary PLBs after 2, 4, 6, and 8 weeks of culture in liquid media. 8 out of 10 ISSR markers produced a total of 275 clear and reproducible bands with mean of 6.9 bands per primer. The secondary PLBs produced during sub-culturing process of chitosan treated liquid culture were genetically uniform and similar to mother plant. AcademicPres 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29275/1/29275.pdf Samarfard, Samira and Abdul Kadir, Mihdzar and Kadzimin, Saleh and Ravanfar, Seyedali and Mohd Saud, Halimi (2013) Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like bodies as affected by chitosan. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 41 (1). pp. 177-183. ISSN 0255-965X; ESSN: 1842-4309 http://www.notulaebotanicae.ro/index.php/nbha/article/view/8315
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Chitosan is a carbohydrate polymer derivative of chitin which presents in shell of crustaceans. This biopolymer is a non toxic and environmentally friendly, considered as a plant growth stimulator in some plant species. The present study investigates the effects of chitosan and media types on multiplication and genetic stability of Phalaenopsis gigantea protocorm-like bodies (PLBs). PLBs were inoculated in liquid New Dogashima Medium (NDM) and Vacin and Went (VW) supplemented with various concentrations of chitosan (0, 5, 10, 15, 20 and 25 mg/L). The highest PLB multiplication was observed on VW and NDM supplemented with 10 mg/L chitosan with mean number of PLBs 177 and 147, respectively. Chitosan promoted the formation of juvenile leaves and the highest number was observed in NDM supplemented with 20 mg/L chitosan with mean number of 66 leaves after 8 weeks of culture. Genetic stability was assessed among mother plant and secondary PLBs after 2, 4, 6, and 8 weeks of culture in liquid media. 8 out of 10 ISSR markers produced a total of 275 clear and reproducible bands with mean of 6.9 bands per primer. The secondary PLBs produced during sub-culturing process of chitosan treated liquid culture were genetically uniform and similar to mother plant.
format Article
author Samarfard, Samira
Abdul Kadir, Mihdzar
Kadzimin, Saleh
Ravanfar, Seyedali
Mohd Saud, Halimi
spellingShingle Samarfard, Samira
Abdul Kadir, Mihdzar
Kadzimin, Saleh
Ravanfar, Seyedali
Mohd Saud, Halimi
Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like bodies as affected by chitosan
author_facet Samarfard, Samira
Abdul Kadir, Mihdzar
Kadzimin, Saleh
Ravanfar, Seyedali
Mohd Saud, Halimi
author_sort Samarfard, Samira
title Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like bodies as affected by chitosan
title_short Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like bodies as affected by chitosan
title_full Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like bodies as affected by chitosan
title_fullStr Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like bodies as affected by chitosan
title_full_unstemmed Genetic stability of in vitro multiplied Phalaenopsis gigantea protocorm-like bodies as affected by chitosan
title_sort genetic stability of in vitro multiplied phalaenopsis gigantea protocorm-like bodies as affected by chitosan
publisher AcademicPres
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29275/1/29275.pdf
http://psasir.upm.edu.my/id/eprint/29275/
http://www.notulaebotanicae.ro/index.php/nbha/article/view/8315
_version_ 1643829712182575104
score 13.211869

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