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Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment

The objective of this study was to determine the level of plant hormone gibberellic acid (GA3) in paddy due to the treatment of Paclobutrazol (PBZ) treatment using high performance liquid chromatography (HPLC) with UV-vis detection at 208 nm. The separation was achieved using reversed column Crestpa...

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Main Authors: Syahputra, Bambang Surya Adji, Sinniah, Uma Rani, Syed Rastan, Syed Omar, Ismail, Mohd Razi
Format: Article
Language:English
Published: Gadjah Mada University 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29240/1/29240.pdf
http://psasir.upm.edu.my/id/eprint/29240/
http://journal.ugm.ac.id/jfps/article/view/1841
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spelling my.upm.eprints.292402016-04-26T01:38:37Z http://psasir.upm.edu.my/id/eprint/29240/ Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment Syahputra, Bambang Surya Adji Sinniah, Uma Rani Syed Rastan, Syed Omar Ismail, Mohd Razi The objective of this study was to determine the level of plant hormone gibberellic acid (GA3) in paddy due to the treatment of Paclobutrazol (PBZ) treatment using high performance liquid chromatography (HPLC) with UV-vis detection at 208 nm. The separation was achieved using reversed column Crestpak C18 (150 mm x 4.6 mm i.d; 5 µm) at 30 ± 1° C using mobile phase of acetonitrile-water (30:70%; v/v), pH 6.80. The treatment of PBZ with different concentration of 100, 200, 400, and 600 mg/L reduced the concentration of GA3 in paddy. The level of GA3 in paddy treated with 100 mg/L of PBZ did not show significant difference from untreated one. However, the level of GA3 in paddy treated with other concentrations (200, 400, and 600 mg/L) of PBZ was significantly different (P < 0.05) from untreated paddy. Gadjah Mada University 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29240/1/29240.pdf Syahputra, Bambang Surya Adji and Sinniah, Uma Rani and Syed Rastan, Syed Omar and Ismail, Mohd Razi (2013) Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment. Journal of Food and Pharmaceutical Sciences, 1 (1). pp. 14-17. ISSN 2089-7200; ESSN: 2339-0948 http://journal.ugm.ac.id/jfps/article/view/1841
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The objective of this study was to determine the level of plant hormone gibberellic acid (GA3) in paddy due to the treatment of Paclobutrazol (PBZ) treatment using high performance liquid chromatography (HPLC) with UV-vis detection at 208 nm. The separation was achieved using reversed column Crestpak C18 (150 mm x 4.6 mm i.d; 5 µm) at 30 ± 1° C using mobile phase of acetonitrile-water (30:70%; v/v), pH 6.80. The treatment of PBZ with different concentration of 100, 200, 400, and 600 mg/L reduced the concentration of GA3 in paddy. The level of GA3 in paddy treated with 100 mg/L of PBZ did not show significant difference from untreated one. However, the level of GA3 in paddy treated with other concentrations (200, 400, and 600 mg/L) of PBZ was significantly different (P < 0.05) from untreated paddy.
format Article
author Syahputra, Bambang Surya Adji
Sinniah, Uma Rani
Syed Rastan, Syed Omar
Ismail, Mohd Razi
spellingShingle Syahputra, Bambang Surya Adji
Sinniah, Uma Rani
Syed Rastan, Syed Omar
Ismail, Mohd Razi
Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment
author_facet Syahputra, Bambang Surya Adji
Sinniah, Uma Rani
Syed Rastan, Syed Omar
Ismail, Mohd Razi
author_sort Syahputra, Bambang Surya Adji
title Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment
title_short Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment
title_full Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment
title_fullStr Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment
title_full_unstemmed Changes in gibberellic acid (GA3) content in Oryza sativa due to paclobutrazol treatment
title_sort changes in gibberellic acid (ga3) content in oryza sativa due to paclobutrazol treatment
publisher Gadjah Mada University
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29240/1/29240.pdf
http://psasir.upm.edu.my/id/eprint/29240/
http://journal.ugm.ac.id/jfps/article/view/1841
_version_ 1643829701748195328
score 13.211869

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