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Isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against Vibrio species

The emergence of antibiotic-resistant bacterial pathogen has led aquaculture attentions to the use of probiotics as an alternative to antibiotics. This study was conducted to isolate bacterial micro flora from digestive tract of healthy juvenile white shrimp, Litopenaeus vannamei based on antagonist...

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Main Authors: Far, Hadi Zokaei, Saad, Che Roos, Mohd Daud, Hassan, Kamarudin, Mohd Salleh, Fard, Ehsan Ramezani
Format: Article
Language:English
Published: Academic Journals 2013
Online Access:http://psasir.upm.edu.my/id/eprint/29213/1/29213.pdf
http://psasir.upm.edu.my/id/eprint/29213/
http://scialert.net/abstract/?doi=ajava.2013.293.300
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spelling my.upm.eprints.292132016-06-20T07:05:04Z http://psasir.upm.edu.my/id/eprint/29213/ Isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against Vibrio species Far, Hadi Zokaei Saad, Che Roos Mohd Daud, Hassan Kamarudin, Mohd Salleh Fard, Ehsan Ramezani The emergence of antibiotic-resistant bacterial pathogen has led aquaculture attentions to the use of probiotics as an alternative to antibiotics. This study was conducted to isolate bacterial micro flora from digestive tract of healthy juvenile white shrimp, Litopenaeus vannamei based on antagonistic activity against shrimp pathogen, Vibrio parahaemolyticus. In this study, potential probiotic strains were isolated using replica plating method to screen the bacteria with antagonism properties. Six isolates were identified as Pseudomonas sp. using conventional biochemical tests and Biolog GN microplates. In addition three Vibrio species including V. parahaemolyticus, Vibrio alginolyticus and Vibrio cholerae were isolated from moribund shrimp and used as pathogen in this study. The pathogenicity of three Vibrio species was tested on shrimp L. vannamei by injecting 0.1 mL of 107 CFU mL-1 of each pathogen into the third abdominal segment. Among three Vibrio species only V. parahaemolyticus found to be highly virulent to shrimp with 43% cumulated mortality after 10 days. Subsequently, all Pseudomonas sp. isolates were tested for antibacterial activity against three Vibrio species using cross streak assay. Strong antibacterial activity was recorded for Pseudomonas sp. isolates number 5, 7, 15 and 30 against three pathogens. In addition a reasonable antibacterial activity was observed for isolates number 9 and 12. On the basis of great antibacterial activity of Pseudomonas sp. isolates these species may be considered for future challenge experiments in shrimp as a very promising alternative to the use of antibiotics. Academic Journals 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29213/1/29213.pdf Far, Hadi Zokaei and Saad, Che Roos and Mohd Daud, Hassan and Kamarudin, Mohd Salleh and Fard, Ehsan Ramezani (2013) Isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against Vibrio species. Asian Journal of Animal and Veterinary Advances, 8 (2). pp. 293-300. ISSN 1683-9919; ESSN: 1996-3289 http://scialert.net/abstract/?doi=ajava.2013.293.300 10.3923/ajava.2013.293.300
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The emergence of antibiotic-resistant bacterial pathogen has led aquaculture attentions to the use of probiotics as an alternative to antibiotics. This study was conducted to isolate bacterial micro flora from digestive tract of healthy juvenile white shrimp, Litopenaeus vannamei based on antagonistic activity against shrimp pathogen, Vibrio parahaemolyticus. In this study, potential probiotic strains were isolated using replica plating method to screen the bacteria with antagonism properties. Six isolates were identified as Pseudomonas sp. using conventional biochemical tests and Biolog GN microplates. In addition three Vibrio species including V. parahaemolyticus, Vibrio alginolyticus and Vibrio cholerae were isolated from moribund shrimp and used as pathogen in this study. The pathogenicity of three Vibrio species was tested on shrimp L. vannamei by injecting 0.1 mL of 107 CFU mL-1 of each pathogen into the third abdominal segment. Among three Vibrio species only V. parahaemolyticus found to be highly virulent to shrimp with 43% cumulated mortality after 10 days. Subsequently, all Pseudomonas sp. isolates were tested for antibacterial activity against three Vibrio species using cross streak assay. Strong antibacterial activity was recorded for Pseudomonas sp. isolates number 5, 7, 15 and 30 against three pathogens. In addition a reasonable antibacterial activity was observed for isolates number 9 and 12. On the basis of great antibacterial activity of Pseudomonas sp. isolates these species may be considered for future challenge experiments in shrimp as a very promising alternative to the use of antibiotics.
format Article
author Far, Hadi Zokaei
Saad, Che Roos
Mohd Daud, Hassan
Kamarudin, Mohd Salleh
Fard, Ehsan Ramezani
spellingShingle Far, Hadi Zokaei
Saad, Che Roos
Mohd Daud, Hassan
Kamarudin, Mohd Salleh
Fard, Ehsan Ramezani
Isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against Vibrio species
author_facet Far, Hadi Zokaei
Saad, Che Roos
Mohd Daud, Hassan
Kamarudin, Mohd Salleh
Fard, Ehsan Ramezani
author_sort Far, Hadi Zokaei
title Isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against Vibrio species
title_short Isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against Vibrio species
title_full Isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against Vibrio species
title_fullStr Isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against Vibrio species
title_full_unstemmed Isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against Vibrio species
title_sort isolation and identification of bacteria micro flora of white shrimp, litopenaeus vannamei, with antagonistic properties against vibrio species
publisher Academic Journals
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/29213/1/29213.pdf
http://psasir.upm.edu.my/id/eprint/29213/
http://scialert.net/abstract/?doi=ajava.2013.293.300
_version_ 1643829693631168512
score 13.211869

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