Cover Image

Analysis of EXO70C2 expression revealed its specific association with late stages of pollen development

Exocyst is an octameric protein complex that mediates the tethering of secretory vesicles at the plasma membrane for exocytosis. In this study, a 1136 bp promoter fragment of exocyst subunit exo70 family protein C2 (EXO70C2) was fused with the β-glucuronidase (GUS) reporter gene and introduced into...

Full description

Saved in:
Bibliographic Details
Main Author: Lai, Kok Song
Format: Article
Language:English
Published: Springer 2016
Online Access:http://psasir.upm.edu.my/id/eprint/29199/1/Analysis%20of%20EXO70C2%20expression%20revealed%20its%20specific%20association%20with%20late%20stages%20of%20pollen%20development.pdf
http://psasir.upm.edu.my/id/eprint/29199/
http://link.springer.com/article/10.1007%2Fs11240-015-0881-5
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.upm.eprints.29199
record_format eprints
spelling my.upm.eprints.291992016-06-20T07:03:24Z http://psasir.upm.edu.my/id/eprint/29199/ Analysis of EXO70C2 expression revealed its specific association with late stages of pollen development Lai, Kok Song Exocyst is an octameric protein complex that mediates the tethering of secretory vesicles at the plasma membrane for exocytosis. In this study, a 1136 bp promoter fragment of exocyst subunit exo70 family protein C2 (EXO70C2) was fused with the β-glucuronidase (GUS) reporter gene and introduced into Arabidopsis thaliana via Agrobacterium tumefaciens. Detail histochemical analysis of EXO70C2-GUS expression in T3 transgenic A. thaliana lines revealed a strong GUS activity at late stages of pollen development. Strong GUS signals were visible from post meiotic pollen, mature pollen and pollen release stages. There was no detectable expression of EXO70C2-GUS during early pollen development and meiosis stages. Consistent with the GUS assay, EXO70C2 transcript profiling using real-time PCR analysis also showed high expression of EXO70C2 at these late stages of pollen development. Further RNA in situ hybridisation revealed the presence of EXO70C2 signals in pollen and as well as anther tapetum. Taken together, these results indicate that, EXO70C2 is specifically expressed in pollen and anther tapetum during the late stages of pollen development, suggesting its function in regulating essential secretory vesicles to support pollen maturation. Springer 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/29199/1/Analysis%20of%20EXO70C2%20expression%20revealed%20its%20specific%20association%20with%20late%20stages%20of%20pollen%20development.pdf Lai, Kok Song (2016) Analysis of EXO70C2 expression revealed its specific association with late stages of pollen development. Plant Cell, Tissue and Organ Culture, 124 (1). pp. 209-215. ISSN 0167-6857; ESSN: 1573-5044 http://link.springer.com/article/10.1007%2Fs11240-015-0881-5 10.1007/s11240-015-0881-5
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Exocyst is an octameric protein complex that mediates the tethering of secretory vesicles at the plasma membrane for exocytosis. In this study, a 1136 bp promoter fragment of exocyst subunit exo70 family protein C2 (EXO70C2) was fused with the β-glucuronidase (GUS) reporter gene and introduced into Arabidopsis thaliana via Agrobacterium tumefaciens. Detail histochemical analysis of EXO70C2-GUS expression in T3 transgenic A. thaliana lines revealed a strong GUS activity at late stages of pollen development. Strong GUS signals were visible from post meiotic pollen, mature pollen and pollen release stages. There was no detectable expression of EXO70C2-GUS during early pollen development and meiosis stages. Consistent with the GUS assay, EXO70C2 transcript profiling using real-time PCR analysis also showed high expression of EXO70C2 at these late stages of pollen development. Further RNA in situ hybridisation revealed the presence of EXO70C2 signals in pollen and as well as anther tapetum. Taken together, these results indicate that, EXO70C2 is specifically expressed in pollen and anther tapetum during the late stages of pollen development, suggesting its function in regulating essential secretory vesicles to support pollen maturation.
format Article
author Lai, Kok Song
spellingShingle Lai, Kok Song
Analysis of EXO70C2 expression revealed its specific association with late stages of pollen development
author_facet Lai, Kok Song
author_sort Lai, Kok Song
title Analysis of EXO70C2 expression revealed its specific association with late stages of pollen development
title_short Analysis of EXO70C2 expression revealed its specific association with late stages of pollen development
title_full Analysis of EXO70C2 expression revealed its specific association with late stages of pollen development
title_fullStr Analysis of EXO70C2 expression revealed its specific association with late stages of pollen development
title_full_unstemmed Analysis of EXO70C2 expression revealed its specific association with late stages of pollen development
title_sort analysis of exo70c2 expression revealed its specific association with late stages of pollen development
publisher Springer
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/29199/1/Analysis%20of%20EXO70C2%20expression%20revealed%20its%20specific%20association%20with%20late%20stages%20of%20pollen%20development.pdf
http://psasir.upm.edu.my/id/eprint/29199/
http://link.springer.com/article/10.1007%2Fs11240-015-0881-5
_version_ 1643829689483001856
score 13.211869

Similar Items