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An on-line sample pretreatment technique for the HPLC analysis of plant samples.

A continuous-flow, on-line sample pretreatment technique using a silica gel microsyringe extractor has been developed. All steps including extraction, separation, clean-up, and concentration occur in the microsyringe. The overall sample pretreatment process takes <10 min per sample. Different pol...

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Main Authors: Yang, Cui, Ren, Chunyan, Piao, Xiangfan, Narayanan, Kannan, Li, Donghao
Format: Article
Language:English
English
Published: Wiley 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28936/1/An%20on-line%20sample%20pretreatment%20technique%20for%20the%20HPLC%20analysis%20of%20plant%20samples..pdf
http://psasir.upm.edu.my/id/eprint/28936/
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spelling my.upm.eprints.289362015-10-26T02:52:16Z http://psasir.upm.edu.my/id/eprint/28936/ An on-line sample pretreatment technique for the HPLC analysis of plant samples. Yang, Cui Ren, Chunyan Piao, Xiangfan Narayanan, Kannan Li, Donghao A continuous-flow, on-line sample pretreatment technique using a silica gel microsyringe extractor has been developed. All steps including extraction, separation, clean-up, and concentration occur in the microsyringe. The overall sample pretreatment process takes <10 min per sample. Different polarity chemicals in the plant sample are successively extracted and separated, and analyzed in parallel using HPLC–UV and HPLC–UV–MS/MS. Polycyclic aromatic hydrocarbons, alkylphenols, and plant hormones were determined as model compounds for nonpolar, intermediate polarity, and polar fractions, respectively. All the parameters that influence the extraction and separation efficiency of the microsyringe extractor have been optimized and evaluated. Under the optimized conditions, recoveries of target compounds ranged from 78.4 to 101.9%, the RSD was <12.8% and the square of the correlation coefficient was >0.99. Complex plant samples of Sambucus Mandshurica Kitag have been tested using this method. Fluorene, phenanthrene, pyrene, and plant hormones were detected in all the samples, and concentrations ranged from 24.2–34.9, 43.8–67.1, 25.9–29.2, and 14.5∼110.8 ng/g, respectively. Wiley 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28936/1/An%20on-line%20sample%20pretreatment%20technique%20for%20the%20HPLC%20analysis%20of%20plant%20samples..pdf Yang, Cui and Ren, Chunyan and Piao, Xiangfan and Narayanan, Kannan and Li, Donghao (2013) An on-line sample pretreatment technique for the HPLC analysis of plant samples. Journal of Separation Science, 36 (21-22). pp. 3599-3607. ISSN 1615-9306; ESSN: 1615-9314 10.1002/jssc.201300722 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description A continuous-flow, on-line sample pretreatment technique using a silica gel microsyringe extractor has been developed. All steps including extraction, separation, clean-up, and concentration occur in the microsyringe. The overall sample pretreatment process takes <10 min per sample. Different polarity chemicals in the plant sample are successively extracted and separated, and analyzed in parallel using HPLC–UV and HPLC–UV–MS/MS. Polycyclic aromatic hydrocarbons, alkylphenols, and plant hormones were determined as model compounds for nonpolar, intermediate polarity, and polar fractions, respectively. All the parameters that influence the extraction and separation efficiency of the microsyringe extractor have been optimized and evaluated. Under the optimized conditions, recoveries of target compounds ranged from 78.4 to 101.9%, the RSD was <12.8% and the square of the correlation coefficient was >0.99. Complex plant samples of Sambucus Mandshurica Kitag have been tested using this method. Fluorene, phenanthrene, pyrene, and plant hormones were detected in all the samples, and concentrations ranged from 24.2–34.9, 43.8–67.1, 25.9–29.2, and 14.5∼110.8 ng/g, respectively.
format Article
author Yang, Cui
Ren, Chunyan
Piao, Xiangfan
Narayanan, Kannan
Li, Donghao
spellingShingle Yang, Cui
Ren, Chunyan
Piao, Xiangfan
Narayanan, Kannan
Li, Donghao
An on-line sample pretreatment technique for the HPLC analysis of plant samples.
author_facet Yang, Cui
Ren, Chunyan
Piao, Xiangfan
Narayanan, Kannan
Li, Donghao
author_sort Yang, Cui
title An on-line sample pretreatment technique for the HPLC analysis of plant samples.
title_short An on-line sample pretreatment technique for the HPLC analysis of plant samples.
title_full An on-line sample pretreatment technique for the HPLC analysis of plant samples.
title_fullStr An on-line sample pretreatment technique for the HPLC analysis of plant samples.
title_full_unstemmed An on-line sample pretreatment technique for the HPLC analysis of plant samples.
title_sort on-line sample pretreatment technique for the hplc analysis of plant samples.
publisher Wiley
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/28936/1/An%20on-line%20sample%20pretreatment%20technique%20for%20the%20HPLC%20analysis%20of%20plant%20samples..pdf
http://psasir.upm.edu.my/id/eprint/28936/
_version_ 1643829613010354176
score 13.211869

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