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Antioxidant activity and total phenolic content of earthworm paste of Lumbricus rubellus (red worm) and Eudrilus eugenia (African night crawler)

Earthworm paste (EP) has many therapeutic properties such as antioxidant, anti-inflammatory, anticancer and antibacterial activities. Antioxidant properties of the earthworm paste extract may attribute to high phenolic compounds. This study involved quantification of phenolic compounds and evaluatio...

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Main Authors:	Mohammed Aldarraji, Qasim, Halimoon , Normala, Nik Ab. Majid, Nik Muhamad
Format:	Article
Published:	Academic Journals 2013
Online Access:	http://psasir.upm.edu.my/id/eprint/28916/ http://www.academicjournals.org/journal/JEN/article-abstract/B3D0ECC10635
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spelling	my.upm.eprints.289162016-02-24T13:12:31Z http://psasir.upm.edu.my/id/eprint/28916/ Antioxidant activity and total phenolic content of earthworm paste of Lumbricus rubellus (red worm) and Eudrilus eugenia (African night crawler) Mohammed Aldarraji, Qasim Halimoon , Normala Nik Ab. Majid, Nik Muhamad Earthworm paste (EP) has many therapeutic properties such as antioxidant, anti-inflammatory, anticancer and antibacterial activities. Antioxidant properties of the earthworm paste extract may attribute to high phenolic compounds. This study involved quantification of phenolic compounds and evaluation of the antioxidant activity of earthworm paste (EP) from Lumbricus rubellus (red worm) and Eudrilus eugeniae (African night crawler). Three types of solvents were used: 75% methanol, 80% methanol and 80% ethanol. Total phenolic compounds were quantified spectrophotometrically using Folin-Ciocalteu reagent. The antioxidant activity of earthworms paste was evaluated using DPPH (1,1-diphenyl-1-2-picrylhydrazl) method. The extraction of phenolics with 80% ethanol in both species yielded the highest amount of phenolics, 220.6 mg/L, in the African night crawler extract and 247.00 mg/L in the red worm extract. Extraction with 80% methanol yielded 217.00 mg/L of phenolics in the African night crawler extract and 228.00 mg/L in the red worm extract. Extraction with 75% methanol yielded higher amounts in red worm (237.00 mg/L) than in African night crawler (207.00 mg/L). EP of the African night crawler yielded a greater amount of phenolic compounds than the red worm. DPPH assay showed that the inhibition activity of African night crawler (95.23%) was significantly higher than that of the red worm extract (38.26%) in 80% methanol, whereas, in 80% ethanol, the inhibition activity of African night crawler (48.13%) was not significantly different from that of the red worm extract (47.73%). Inhibition of antioxidant activity of African night crawler extract in 75% methanol (48.13%) was higher than that of the red worm extract (47.93%). The study showed that earthworm paste has notable amounts of phenolic compounds that have significant antioxidant activity. Academic Journals 2013 Article PeerReviewed Mohammed Aldarraji, Qasim and Halimoon , Normala and Nik Ab. Majid, Nik Muhamad (2013) Antioxidant activity and total phenolic content of earthworm paste of Lumbricus rubellus (red worm) and Eudrilus eugenia (African night crawler). Journal of Entomology and Nematology, 5 (3). pp. 33-37. ISSN 2006-9855 http://www.academicjournals.org/journal/JEN/article-abstract/B3D0ECC10635
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
description	Earthworm paste (EP) has many therapeutic properties such as antioxidant, anti-inflammatory, anticancer and antibacterial activities. Antioxidant properties of the earthworm paste extract may attribute to high phenolic compounds. This study involved quantification of phenolic compounds and evaluation of the antioxidant activity of earthworm paste (EP) from Lumbricus rubellus (red worm) and Eudrilus eugeniae (African night crawler). Three types of solvents were used: 75% methanol, 80% methanol and 80% ethanol. Total phenolic compounds were quantified spectrophotometrically using Folin-Ciocalteu reagent. The antioxidant activity of earthworms paste was evaluated using DPPH (1,1-diphenyl-1-2-picrylhydrazl) method. The extraction of phenolics with 80% ethanol in both species yielded the highest amount of phenolics, 220.6 mg/L, in the African night crawler extract and 247.00 mg/L in the red worm extract. Extraction with 80% methanol yielded 217.00 mg/L of phenolics in the African night crawler extract and 228.00 mg/L in the red worm extract. Extraction with 75% methanol yielded higher amounts in red worm (237.00 mg/L) than in African night crawler (207.00 mg/L). EP of the African night crawler yielded a greater amount of phenolic compounds than the red worm. DPPH assay showed that the inhibition activity of African night crawler (95.23%) was significantly higher than that of the red worm extract (38.26%) in 80% methanol, whereas, in 80% ethanol, the inhibition activity of African night crawler (48.13%) was not significantly different from that of the red worm extract (47.73%). Inhibition of antioxidant activity of African night crawler extract in 75% methanol (48.13%) was higher than that of the red worm extract (47.93%). The study showed that earthworm paste has notable amounts of phenolic compounds that have significant antioxidant activity.
format	Article
author	Mohammed Aldarraji, Qasim Halimoon , Normala Nik Ab. Majid, Nik Muhamad
spellingShingle	Mohammed Aldarraji, Qasim Halimoon , Normala Nik Ab. Majid, Nik Muhamad Antioxidant activity and total phenolic content of earthworm paste of Lumbricus rubellus (red worm) and Eudrilus eugenia (African night crawler)
author_facet	Mohammed Aldarraji, Qasim Halimoon , Normala Nik Ab. Majid, Nik Muhamad
author_sort	Mohammed Aldarraji, Qasim
title	Antioxidant activity and total phenolic content of earthworm paste of Lumbricus rubellus (red worm) and Eudrilus eugenia (African night crawler)
title_short	Antioxidant activity and total phenolic content of earthworm paste of Lumbricus rubellus (red worm) and Eudrilus eugenia (African night crawler)
title_full	Antioxidant activity and total phenolic content of earthworm paste of Lumbricus rubellus (red worm) and Eudrilus eugenia (African night crawler)
title_fullStr	Antioxidant activity and total phenolic content of earthworm paste of Lumbricus rubellus (red worm) and Eudrilus eugenia (African night crawler)
title_full_unstemmed	Antioxidant activity and total phenolic content of earthworm paste of Lumbricus rubellus (red worm) and Eudrilus eugenia (African night crawler)
title_sort	antioxidant activity and total phenolic content of earthworm paste of lumbricus rubellus (red worm) and eudrilus eugenia (african night crawler)
publisher	Academic Journals
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/28916/ http://www.academicjournals.org/journal/JEN/article-abstract/B3D0ECC10635
_version_	1643829607079608320
score	13.211869

Antioxidant activity and total phenolic content of earthworm paste of Lumbricus rubellus (red worm) and Eudrilus eugenia (African night crawler)

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