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Comparison of particle bombardment-transformation parameters between stem and calli explants of Aglaonema simplex

Aquatic plant posses a very interesting stress tolerance mechanism compared to terrestrial plants. However, lack of aquatic mutants available is a hindrance to explore the cellular mechanism that governs various adaptations between these two types of plants. Therefore, the aim of this study is to pr...

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Main Authors: Ma, Nyuk Ling, Cha, Thye San, Mahmood, Maziah, Ahmad, Aziz
Format: Article
Language:English
English
Published: Academic Journals 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28136/1/Comparison%20of%20Partial%20Least%20Squares%20and%20Artificial%20Neural%20Network%20for%20the%20prediction%20of%20antioxidant%20activity%20in%20extract%20of%20pegaga.pdf
http://psasir.upm.edu.my/id/eprint/28136/
http://www.scialert.net/archivedetails.php?issn=1816-4927&issueno=46
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spelling my.upm.eprints.281362015-10-08T00:24:08Z http://psasir.upm.edu.my/id/eprint/28136/ Comparison of particle bombardment-transformation parameters between stem and calli explants of Aglaonema simplex Ma, Nyuk Ling Cha, Thye San Mahmood, Maziah Ahmad, Aziz Aquatic plant posses a very interesting stress tolerance mechanism compared to terrestrial plants. However, lack of aquatic mutants available is a hindrance to explore the cellular mechanism that governs various adaptations between these two types of plants. Therefore, the aim of this study is to provide the optimum parameters for the genetic transformation of native aquatic plant in tropical country, Aglaonema simplex, by using stem and callus tissue. Explants were bombarded with various sizes of gold particles coated with 35 sec promoter constructed into 35 sec-sGFP-TYG-nos (puc18) plasmid harbouring green fluorescent protein. High transient expression was obtained when the tissues were bombarded twice by gold particles coated with spermidine under acceleration pressure of 1100-1350 psi and with the vacuum pressure set to 24 Hg. The calli were found susceptible to high pressure impact, showing better result at a longer bombardment distance (12 cm) of stopping screen to explants by the use of gold particle of smaller size (1 μm). In contrast, the optimal bombardment distance shown by stem tissues was achieved at a 6 cm distance by the use of gold particle of 1.6 μm. PCR and PCR-southern blot analyses exhibited that the gene was integrated into the genome of transformants and this indicates that the protocol can be used for genetic engineering purpose in the future. Academic Journals 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28136/1/Comparison%20of%20Partial%20Least%20Squares%20and%20Artificial%20Neural%20Network%20for%20the%20prediction%20of%20antioxidant%20activity%20in%20extract%20of%20pegaga.pdf Ma, Nyuk Ling and Cha, Thye San and Mahmood, Maziah and Ahmad, Aziz (2013) Comparison of particle bombardment-transformation parameters between stem and calli explants of Aglaonema simplex. Journal of Fisheries and Aquatic Science, 8 (4). pp. 544-552. ISSN 1816-4927; ESSN: 1996-0751 http://www.scialert.net/archivedetails.php?issn=1816-4927&issueno=46 10.3923/jfas.2013.544.552 English
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
English
description Aquatic plant posses a very interesting stress tolerance mechanism compared to terrestrial plants. However, lack of aquatic mutants available is a hindrance to explore the cellular mechanism that governs various adaptations between these two types of plants. Therefore, the aim of this study is to provide the optimum parameters for the genetic transformation of native aquatic plant in tropical country, Aglaonema simplex, by using stem and callus tissue. Explants were bombarded with various sizes of gold particles coated with 35 sec promoter constructed into 35 sec-sGFP-TYG-nos (puc18) plasmid harbouring green fluorescent protein. High transient expression was obtained when the tissues were bombarded twice by gold particles coated with spermidine under acceleration pressure of 1100-1350 psi and with the vacuum pressure set to 24 Hg. The calli were found susceptible to high pressure impact, showing better result at a longer bombardment distance (12 cm) of stopping screen to explants by the use of gold particle of smaller size (1 μm). In contrast, the optimal bombardment distance shown by stem tissues was achieved at a 6 cm distance by the use of gold particle of 1.6 μm. PCR and PCR-southern blot analyses exhibited that the gene was integrated into the genome of transformants and this indicates that the protocol can be used for genetic engineering purpose in the future.
format Article
author Ma, Nyuk Ling
Cha, Thye San
Mahmood, Maziah
Ahmad, Aziz
spellingShingle Ma, Nyuk Ling
Cha, Thye San
Mahmood, Maziah
Ahmad, Aziz
Comparison of particle bombardment-transformation parameters between stem and calli explants of Aglaonema simplex
author_facet Ma, Nyuk Ling
Cha, Thye San
Mahmood, Maziah
Ahmad, Aziz
author_sort Ma, Nyuk Ling
title Comparison of particle bombardment-transformation parameters between stem and calli explants of Aglaonema simplex
title_short Comparison of particle bombardment-transformation parameters between stem and calli explants of Aglaonema simplex
title_full Comparison of particle bombardment-transformation parameters between stem and calli explants of Aglaonema simplex
title_fullStr Comparison of particle bombardment-transformation parameters between stem and calli explants of Aglaonema simplex
title_full_unstemmed Comparison of particle bombardment-transformation parameters between stem and calli explants of Aglaonema simplex
title_sort comparison of particle bombardment-transformation parameters between stem and calli explants of aglaonema simplex
publisher Academic Journals
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/28136/1/Comparison%20of%20Partial%20Least%20Squares%20and%20Artificial%20Neural%20Network%20for%20the%20prediction%20of%20antioxidant%20activity%20in%20extract%20of%20pegaga.pdf
http://psasir.upm.edu.my/id/eprint/28136/
http://www.scialert.net/archivedetails.php?issn=1816-4927&issueno=46
_version_ 1643829380327145472
score 13.211869

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