Effect of condensed tannins on bovine rumen protist diversity based on 18S rRNA gene sequences

Molecular diversity of protists from bovine rumen fluid incubated with condensed tannins of Leucaena leucocephala hybrid-Rendang at 20 mg/500 mg dry matter (treatment) or without condensed tannins (control) was investigated using 18S rRNA gene library. Clones from the control library were distribute...

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Bibliographic Details
Main Authors: Tan, Hui Yin, Sieo, Chin Chin, Abdullah, Norhani, Liang, Juan Boo, Huang, Xiao Dan, Ho, Yin Wan
Format: Article
Language:English
Published: International Society of Protistologists 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28101/1/Effect%20of%20condensed%20tannins%20on%20bovine%20rumen%20protist%20diversity%20based%20on%2018S%20rRNA%20gene%20sequences.pdf
http://psasir.upm.edu.my/id/eprint/28101/
http://onlinelibrary.wiley.com/wol1/doi/10.1111/jeu.12011/abstract
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Summary:Molecular diversity of protists from bovine rumen fluid incubated with condensed tannins of Leucaena leucocephala hybrid-Rendang at 20 mg/500 mg dry matter (treatment) or without condensed tannins (control) was investigated using 18S rRNA gene library. Clones from the control library were distributed within nine genera, but clones from the condensed tannin treatment clone library were related to only six genera. Diversity estimators such as abundance-based coverage estimation and Chao1 showed significant differences between the two libraries, although no differences were found based on Shannon–Weaver index and Libshuff.