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Production of ligninolytic enzymes by newly isolated bacteria from palm oil plantation soils

Three aerobic lignin-degrading bacterial strains were isolated from palm oil plantation soils. The bacterial isolates were screened using a selective nutrient medium of minimum salt media (MSM), with kraft lignin as lignin substrate and methylene blue as the ligninolytic dye indicator. The newly iso...

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Main Authors: Abdul Rahman, Nor Hashimah, Abdul Rahman, Nor'aini, Abd. Aziz, Suraini, Hassan, Mohd Ali
Format: Article
Language:English
Published: North Carolina State University 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28084/1/Production%20of%20ligninolytic%20enzymes%20by%20newly%20isolated%20bacteria%20from%20palm%20oil%20plantation%20soils.pdf
http://psasir.upm.edu.my/id/eprint/28084/
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_08_4_6136_Abdul_Rahman_Production_Ligninolytic_Enzymes
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spelling my.upm.eprints.280842016-09-02T08:31:21Z http://psasir.upm.edu.my/id/eprint/28084/ Production of ligninolytic enzymes by newly isolated bacteria from palm oil plantation soils Abdul Rahman, Nor Hashimah Abdul Rahman, Nor'aini Abd. Aziz, Suraini Hassan, Mohd Ali Three aerobic lignin-degrading bacterial strains were isolated from palm oil plantation soils. The bacterial isolates were screened using a selective nutrient medium of minimum salt media (MSM), with kraft lignin as lignin substrate and methylene blue as the ligninolytic dye indicator. The newly isolated bacterial strains SHC1, SHC2, and SHC3 were found to have the potential to tolerate high concentrations of kraft lignin and produced all three main ligninolytic enzymes (lignin peroxidase, manganese peroxidase, and laccase); these strains may therefore be useful in the degradation of lignin in oil palm empty fruit bunch biomass. The production of ligninolytic enzymes was carried out by means of submerged fermentation for 7 days using 2 mm of oil palm empty fruit bunch (OPEFB) fiber as a substrate. These bacterial isolates were characterized using biochemical tests from Biolog and identified using 16S rRNA gene sequencing analysis, which identified the strains SHC1, SHC2, and SHC3 as Bacillus sp., Ochrobactrum sp., and Leucobacter sp., respectively with 99% sequence similarity. Bacillus sp. SHC1 produced the highest manganese peroxidase (MnP) of 2313.4 U/L on the third day and the highest lignin peroxidase (LiP) of 209.30 U/L on the fifth day of fermentation. The optimum pH and temperature for the production of ligninolytic enzymes by Bacillus sp. SHC1 were pH 8 and 30°C. North Carolina State University 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28084/1/Production%20of%20ligninolytic%20enzymes%20by%20newly%20isolated%20bacteria%20from%20palm%20oil%20plantation%20soils.pdf Abdul Rahman, Nor Hashimah and Abdul Rahman, Nor'aini and Abd. Aziz, Suraini and Hassan, Mohd Ali (2013) Production of ligninolytic enzymes by newly isolated bacteria from palm oil plantation soils. BioResources, 8 (4). pp. 6136-6150. ISSN 1930-2126 http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_08_4_6136_Abdul_Rahman_Production_Ligninolytic_Enzymes
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Three aerobic lignin-degrading bacterial strains were isolated from palm oil plantation soils. The bacterial isolates were screened using a selective nutrient medium of minimum salt media (MSM), with kraft lignin as lignin substrate and methylene blue as the ligninolytic dye indicator. The newly isolated bacterial strains SHC1, SHC2, and SHC3 were found to have the potential to tolerate high concentrations of kraft lignin and produced all three main ligninolytic enzymes (lignin peroxidase, manganese peroxidase, and laccase); these strains may therefore be useful in the degradation of lignin in oil palm empty fruit bunch biomass. The production of ligninolytic enzymes was carried out by means of submerged fermentation for 7 days using 2 mm of oil palm empty fruit bunch (OPEFB) fiber as a substrate. These bacterial isolates were characterized using biochemical tests from Biolog and identified using 16S rRNA gene sequencing analysis, which identified the strains SHC1, SHC2, and SHC3 as Bacillus sp., Ochrobactrum sp., and Leucobacter sp., respectively with 99% sequence similarity. Bacillus sp. SHC1 produced the highest manganese peroxidase (MnP) of 2313.4 U/L on the third day and the highest lignin peroxidase (LiP) of 209.30 U/L on the fifth day of fermentation. The optimum pH and temperature for the production of ligninolytic enzymes by Bacillus sp. SHC1 were pH 8 and 30°C.
format Article
author Abdul Rahman, Nor Hashimah
Abdul Rahman, Nor'aini
Abd. Aziz, Suraini
Hassan, Mohd Ali
spellingShingle Abdul Rahman, Nor Hashimah
Abdul Rahman, Nor'aini
Abd. Aziz, Suraini
Hassan, Mohd Ali
Production of ligninolytic enzymes by newly isolated bacteria from palm oil plantation soils
author_facet Abdul Rahman, Nor Hashimah
Abdul Rahman, Nor'aini
Abd. Aziz, Suraini
Hassan, Mohd Ali
author_sort Abdul Rahman, Nor Hashimah
title Production of ligninolytic enzymes by newly isolated bacteria from palm oil plantation soils
title_short Production of ligninolytic enzymes by newly isolated bacteria from palm oil plantation soils
title_full Production of ligninolytic enzymes by newly isolated bacteria from palm oil plantation soils
title_fullStr Production of ligninolytic enzymes by newly isolated bacteria from palm oil plantation soils
title_full_unstemmed Production of ligninolytic enzymes by newly isolated bacteria from palm oil plantation soils
title_sort production of ligninolytic enzymes by newly isolated bacteria from palm oil plantation soils
publisher North Carolina State University
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/28084/1/Production%20of%20ligninolytic%20enzymes%20by%20newly%20isolated%20bacteria%20from%20palm%20oil%20plantation%20soils.pdf
http://psasir.upm.edu.my/id/eprint/28084/
http://ojs.cnr.ncsu.edu/index.php/BioRes/article/view/BioRes_08_4_6136_Abdul_Rahman_Production_Ligninolytic_Enzymes
_version_ 1643829364813463552
score 13.211869

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