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Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride

Biofilm formation can lead to various consequences in the food processing line such as contamination and equipment breakdowns. Since formation of biofilm can occur in various conditions; this study was carried out using L. monocytogenes ATCC 19112 and its biofilm formation ability tested under vario...

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Main Authors: Lee, Hai Yen, Chai, Lay Ching, Pui, Chai Fung, Mustafa, Shuhaimi, Cheah, Yoke Kqueen, Nishibuchi, Mitsuaki, Radu, Son
Format: Article
Language:English
Published: Brazilian Society for Microbiology 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28067/1/28067.pdf
http://psasir.upm.edu.my/id/eprint/28067/
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spelling my.upm.eprints.280672016-04-21T07:33:07Z http://psasir.upm.edu.my/id/eprint/28067/ Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride Lee, Hai Yen Chai, Lay Ching Pui, Chai Fung Mustafa, Shuhaimi Cheah, Yoke Kqueen Nishibuchi, Mitsuaki Radu, Son Biofilm formation can lead to various consequences in the food processing line such as contamination and equipment breakdowns. Since formation of biofilm can occur in various conditions; this study was carried out using L. monocytogenes ATCC 19112 and its biofilm formation ability tested under various concentrations of sodium chloride and temperatures. Cultures of L. monocytogenes ATCC 19112 were placed in 96-well microtitre plate containing concentration of sodium chloride from 1–10% (w/v) and incubated at different temperature of 4°C, 30°C and 45°C for up to 60 h. Absorbance reading of crystal violet staining showed the density of biofilm formed in the 96-well microtitre plates was significantly higher when incubated in 4°C. The formation of biofilm also occurs at a faster rate at 4°C and higher optical density (OD 570 nm) was observed at 45°C. This shows that storage under formation of biofilm that may lead to a higher contamination along the processing line in the food industry. Formation of biofilm was found to be more dependent on temperature compared to sodium chloride stress. Brazilian Society for Microbiology 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28067/1/28067.pdf Lee, Hai Yen and Chai, Lay Ching and Pui, Chai Fung and Mustafa, Shuhaimi and Cheah, Yoke Kqueen and Nishibuchi, Mitsuaki and Radu, Son (2013) Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride. Brazilian Journal of Microbiology, 44 (1). pp. 51-55. ISSN 1517-8382; ESSN: 1678-4405 10.1590/S1517-83822013005000004
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Biofilm formation can lead to various consequences in the food processing line such as contamination and equipment breakdowns. Since formation of biofilm can occur in various conditions; this study was carried out using L. monocytogenes ATCC 19112 and its biofilm formation ability tested under various concentrations of sodium chloride and temperatures. Cultures of L. monocytogenes ATCC 19112 were placed in 96-well microtitre plate containing concentration of sodium chloride from 1–10% (w/v) and incubated at different temperature of 4°C, 30°C and 45°C for up to 60 h. Absorbance reading of crystal violet staining showed the density of biofilm formed in the 96-well microtitre plates was significantly higher when incubated in 4°C. The formation of biofilm also occurs at a faster rate at 4°C and higher optical density (OD 570 nm) was observed at 45°C. This shows that storage under formation of biofilm that may lead to a higher contamination along the processing line in the food industry. Formation of biofilm was found to be more dependent on temperature compared to sodium chloride stress.
format Article
author Lee, Hai Yen
Chai, Lay Ching
Pui, Chai Fung
Mustafa, Shuhaimi
Cheah, Yoke Kqueen
Nishibuchi, Mitsuaki
Radu, Son
spellingShingle Lee, Hai Yen
Chai, Lay Ching
Pui, Chai Fung
Mustafa, Shuhaimi
Cheah, Yoke Kqueen
Nishibuchi, Mitsuaki
Radu, Son
Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride
author_facet Lee, Hai Yen
Chai, Lay Ching
Pui, Chai Fung
Mustafa, Shuhaimi
Cheah, Yoke Kqueen
Nishibuchi, Mitsuaki
Radu, Son
author_sort Lee, Hai Yen
title Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride
title_short Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride
title_full Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride
title_fullStr Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride
title_full_unstemmed Formation of biofilm by Listeria monocytogenes ATCC 19112 at different incubation temperatures and concentrations of sodium chloride
title_sort formation of biofilm by listeria monocytogenes atcc 19112 at different incubation temperatures and concentrations of sodium chloride
publisher Brazilian Society for Microbiology
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/28067/1/28067.pdf
http://psasir.upm.edu.my/id/eprint/28067/
_version_ 1643829359777153024
score 13.211869

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