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An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli

A stirred tank bioreactor (STB) integrated with an expanded bed adsorption (EBA) system containing anion-exchange resin (Diaion WA30) was developed for in situ removal of acetate to increase the production of α-interferon-2b (α-PrIFN-2b) by Escherichia coli (E. coli). Although the total acetate (9.7...

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Main Authors: Tan, Joo Shun, Ling, Tau Chuan, Mustafa, Shuhaimi, Tam, Yew Joon, Ramanan, Ramakrishnan Nagasundara, Ariff, Arbakariya
Format: Article
Language:English
Published: Elsevier 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28066/1/An%20integrated%20bioreactor-expanded%20bed%20adsorption%20system%20for%20the%20removal%20of%20acetate%20to%20enhance%20the%20production%20of%20alpha-interferon-2b%20by%20Escherichia%20coli.pdf
http://psasir.upm.edu.my/id/eprint/28066/
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spelling my.upm.eprints.280662016-04-21T06:08:18Z http://psasir.upm.edu.my/id/eprint/28066/ An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli Tan, Joo Shun Ling, Tau Chuan Mustafa, Shuhaimi Tam, Yew Joon Ramanan, Ramakrishnan Nagasundara Ariff, Arbakariya A stirred tank bioreactor (STB) integrated with an expanded bed adsorption (EBA) system containing anion-exchange resin (Diaion WA30) was developed for in situ removal of acetate to increase the production of α-interferon-2b (α-PrIFN-2b) by Escherichia coli (E. coli). Although the total acetate (9.79 g/L) secreted by E. coli in the integrated STB/EBA system was higher than that in a bioreactor with dispersed resin or a conventional batch bioreactor, cell growth (14.97 g/L) and α-PrIFN-2b production (867.4 μg/L) were significantly improved owing to the high efficiency of acetate removal from the culture. The production of α-PrIFN-2b in the integrated STB/EBA system was improved by 3-fold and 1.4-fold over that obtained in a conventional batch bioreactor and a bioreactor containing dispersed resins, respectively. Elsevier 2013-04 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28066/1/An%20integrated%20bioreactor-expanded%20bed%20adsorption%20system%20for%20the%20removal%20of%20acetate%20to%20enhance%20the%20production%20of%20alpha-interferon-2b%20by%20Escherichia%20coli.pdf Tan, Joo Shun and Ling, Tau Chuan and Mustafa, Shuhaimi and Tam, Yew Joon and Ramanan, Ramakrishnan Nagasundara and Ariff, Arbakariya (2013) An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli. Process Biochemistry, 48 (4). pp. 551-558. ISSN 1359-5113; ESSN: 1873-3298 10.1016/j.procbio.2013.02.024
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description A stirred tank bioreactor (STB) integrated with an expanded bed adsorption (EBA) system containing anion-exchange resin (Diaion WA30) was developed for in situ removal of acetate to increase the production of α-interferon-2b (α-PrIFN-2b) by Escherichia coli (E. coli). Although the total acetate (9.79 g/L) secreted by E. coli in the integrated STB/EBA system was higher than that in a bioreactor with dispersed resin or a conventional batch bioreactor, cell growth (14.97 g/L) and α-PrIFN-2b production (867.4 μg/L) were significantly improved owing to the high efficiency of acetate removal from the culture. The production of α-PrIFN-2b in the integrated STB/EBA system was improved by 3-fold and 1.4-fold over that obtained in a conventional batch bioreactor and a bioreactor containing dispersed resins, respectively.
format Article
author Tan, Joo Shun
Ling, Tau Chuan
Mustafa, Shuhaimi
Tam, Yew Joon
Ramanan, Ramakrishnan Nagasundara
Ariff, Arbakariya
spellingShingle Tan, Joo Shun
Ling, Tau Chuan
Mustafa, Shuhaimi
Tam, Yew Joon
Ramanan, Ramakrishnan Nagasundara
Ariff, Arbakariya
An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli
author_facet Tan, Joo Shun
Ling, Tau Chuan
Mustafa, Shuhaimi
Tam, Yew Joon
Ramanan, Ramakrishnan Nagasundara
Ariff, Arbakariya
author_sort Tan, Joo Shun
title An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli
title_short An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli
title_full An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli
title_fullStr An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli
title_full_unstemmed An integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by Escherichia coli
title_sort integrated bioreactor-expanded bed adsorption system for the removal of acetate to enhance the production of alpha-interferon-2b by escherichia coli
publisher Elsevier
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/28066/1/An%20integrated%20bioreactor-expanded%20bed%20adsorption%20system%20for%20the%20removal%20of%20acetate%20to%20enhance%20the%20production%20of%20alpha-interferon-2b%20by%20Escherichia%20coli.pdf
http://psasir.upm.edu.my/id/eprint/28066/
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score 13.211869

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