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1-aminocyclopropane-1-carboxylate oxidase 2 reduction effects on physical and physiological responses of Eksotika papaya

The Malaysian Eksotika papaya (Carica papaya L.) has poor keeping quality due to its fast ripening attribute, which leads to post-harvest losses. This study is aimed at extending the shelf life of this perishable Eksotika fruit using antisense technology. A total of 6,000 Eksotika somatic embryogeni...

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Main Authors: Sekeli, Rogayah, Abdullah, Janna Ong, Muda, Pauziah, Namasivayam, Parameswari, Abu Bakar, Umi Kalsom, Sew, Yun Shin
Format: Article
Language:English
Published: Taylor & Francis 2013
Online Access:http://psasir.upm.edu.my/id/eprint/28057/1/28057.pdf
http://psasir.upm.edu.my/id/eprint/28057/
http://www.tandfonline.com/doi/abs/10.1080/15427528.2013.795205
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spelling my.upm.eprints.280572016-04-25T07:14:52Z http://psasir.upm.edu.my/id/eprint/28057/ 1-aminocyclopropane-1-carboxylate oxidase 2 reduction effects on physical and physiological responses of Eksotika papaya Sekeli, Rogayah Abdullah, Janna Ong Muda, Pauziah Namasivayam, Parameswari Abu Bakar, Umi Kalsom Sew, Yun Shin The Malaysian Eksotika papaya (Carica papaya L.) has poor keeping quality due to its fast ripening attribute, which leads to post-harvest losses. This study is aimed at extending the shelf life of this perishable Eksotika fruit using antisense technology. A total of 6,000 Eksotika somatic embryogenic calli was transformed with the antisense 1-aminocyclopropane-1-carboxylic acid oxidase 2 gene (ACO2) construct, and 46 PCR-positive putative transformants were obtained. Gene expression analysis using real-time PCR on the 46 regenerated putative transgenic lines revealed that 42 showed down-regulation of the ACO2 gene with two- to five-fold differences among the lines. Out of 22 independently selected transformed lines grown under net house conditions, 16 harbored a single copy of the transgene. Physical stature of the transgenic plants was not significantly different from that of the non-transformed seed-derived papaya plants. Physiological evaluations of the transgenic fruits showed a 15-day delay in ripening compared with 4 days of the non-transformed seed-derived papaya fruits. The total soluble solid (TSS) of the transgenic fruits was comparable to that of the non-transformed seed-derived fruits with similar 11-15°Brix, implying the transgenes did not affect the TSS content. Taylor & Francis 2013 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28057/1/28057.pdf Sekeli, Rogayah and Abdullah, Janna Ong and Muda, Pauziah and Namasivayam, Parameswari and Abu Bakar, Umi Kalsom and Sew, Yun Shin (2013) 1-aminocyclopropane-1-carboxylate oxidase 2 reduction effects on physical and physiological responses of Eksotika papaya. Journal of Crop Improvement, 27 (5). pp. 487-506. ISSN 1542-7528; ESSN: 1542-7536 http://www.tandfonline.com/doi/abs/10.1080/15427528.2013.795205 10.1080/15427528.2013.795205
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description The Malaysian Eksotika papaya (Carica papaya L.) has poor keeping quality due to its fast ripening attribute, which leads to post-harvest losses. This study is aimed at extending the shelf life of this perishable Eksotika fruit using antisense technology. A total of 6,000 Eksotika somatic embryogenic calli was transformed with the antisense 1-aminocyclopropane-1-carboxylic acid oxidase 2 gene (ACO2) construct, and 46 PCR-positive putative transformants were obtained. Gene expression analysis using real-time PCR on the 46 regenerated putative transgenic lines revealed that 42 showed down-regulation of the ACO2 gene with two- to five-fold differences among the lines. Out of 22 independently selected transformed lines grown under net house conditions, 16 harbored a single copy of the transgene. Physical stature of the transgenic plants was not significantly different from that of the non-transformed seed-derived papaya plants. Physiological evaluations of the transgenic fruits showed a 15-day delay in ripening compared with 4 days of the non-transformed seed-derived papaya fruits. The total soluble solid (TSS) of the transgenic fruits was comparable to that of the non-transformed seed-derived fruits with similar 11-15°Brix, implying the transgenes did not affect the TSS content.
format Article
author Sekeli, Rogayah
Abdullah, Janna Ong
Muda, Pauziah
Namasivayam, Parameswari
Abu Bakar, Umi Kalsom
Sew, Yun Shin
spellingShingle Sekeli, Rogayah
Abdullah, Janna Ong
Muda, Pauziah
Namasivayam, Parameswari
Abu Bakar, Umi Kalsom
Sew, Yun Shin
1-aminocyclopropane-1-carboxylate oxidase 2 reduction effects on physical and physiological responses of Eksotika papaya
author_facet Sekeli, Rogayah
Abdullah, Janna Ong
Muda, Pauziah
Namasivayam, Parameswari
Abu Bakar, Umi Kalsom
Sew, Yun Shin
author_sort Sekeli, Rogayah
title 1-aminocyclopropane-1-carboxylate oxidase 2 reduction effects on physical and physiological responses of Eksotika papaya
title_short 1-aminocyclopropane-1-carboxylate oxidase 2 reduction effects on physical and physiological responses of Eksotika papaya
title_full 1-aminocyclopropane-1-carboxylate oxidase 2 reduction effects on physical and physiological responses of Eksotika papaya
title_fullStr 1-aminocyclopropane-1-carboxylate oxidase 2 reduction effects on physical and physiological responses of Eksotika papaya
title_full_unstemmed 1-aminocyclopropane-1-carboxylate oxidase 2 reduction effects on physical and physiological responses of Eksotika papaya
title_sort 1-aminocyclopropane-1-carboxylate oxidase 2 reduction effects on physical and physiological responses of eksotika papaya
publisher Taylor & Francis
publishDate 2013
url http://psasir.upm.edu.my/id/eprint/28057/1/28057.pdf
http://psasir.upm.edu.my/id/eprint/28057/
http://www.tandfonline.com/doi/abs/10.1080/15427528.2013.795205
_version_ 1643829356778225664
score 13.211869

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