Staff View: Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta)

Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta)

Vanadium-dependent haloperoxidases belong to a class of vanadium enzymes that may have potential industrial and pharmaceutical applications due to their high stability. In this study, the 5′-flanking genomic sequence and complete reading frame encoding vanadium-dependent bromoperoxidase (GcVBPO1) wa...

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Main Authors:	Baharum, Hariyanti, Chu, W. C., Teo, Swee Sen, Ng, Kim Yong, Abdul Rahim, Raha, Ho, Chai Ling
Format:	Article
Language:	English
Published:	Elsevier 2013
Online Access:	http://psasir.upm.edu.my/id/eprint/28037/1/28037.pdf http://psasir.upm.edu.my/id/eprint/28037/ http://www.sciencedirect.com/science/article/pii/S0031942213001556
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spelling	my.upm.eprints.280372016-04-25T07:05:06Z http://psasir.upm.edu.my/id/eprint/28037/ Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta) Baharum, Hariyanti Chu, W. C. Teo, Swee Sen Ng, Kim Yong Abdul Rahim, Raha Ho, Chai Ling Vanadium-dependent haloperoxidases belong to a class of vanadium enzymes that may have potential industrial and pharmaceutical applications due to their high stability. In this study, the 5′-flanking genomic sequence and complete reading frame encoding vanadium-dependent bromoperoxidase (GcVBPO1) was cloned from the red seaweed, Fracilaria changii, and the recombinant protein was biochemically characterized. The deduced amino acid sequence of GcVBPO1 is 1818 nucleotides in length, sharing 49% identity with the vanadium-dependent bromoperoxidases from Corralina officinalis and Cor. pilulifera, respectively. The amino acid residues associated with the binding site of vanadate cofactor were found to be conserved. The Km value of recombinant GcVBPO1 for Br− was 4.69 mM, while its Vmax was 10.61 μkat mg−1 at pH 7. Substitution of Arg379 with His379 in the recombinant protein caused a lower affinity for Br−, while substitution of Arg379 with Phe379 not only increased its affinity for Br− but also enabled the mutant enzyme to oxidize Cl−. The mutant Arg379Phe was also found to have a lower affinity for I−, as compared to the wild-type GcVBPO1 and mutant Arg379His. In addition, the Arg379Phe mutant has a slightly higher affinity for H2O2 compared to the wild-type GcVBPO1. Multiple cis-acting regulatory elements associated with light response, hormone signaling, and meristem expression were detected at the 5′-flanking genomic sequence of GcVBPO1. The transcript abundance of GcVBPO1 was relatively higher in seaweed samples treated with 50 parts per thousand (ppt) artificial seawater (ASW) compared to those treated in 10 and 30 ppt ASW, in support of its role in the abiotic stress response of seaweed. Elsevier 2013-08 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/28037/1/28037.pdf Baharum, Hariyanti and Chu, W. C. and Teo, Swee Sen and Ng, Kim Yong and Abdul Rahim, Raha and Ho, Chai Ling (2013) Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta). Phytochemistry, 92. pp. 49-59. ISSN 0031-9422; ESSN: 1873-3700 http://www.sciencedirect.com/science/article/pii/S0031942213001556 10.1016/j.phytochem.2013.04.014
institution	Universiti Putra Malaysia
building	UPM Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Putra Malaysia
content_source	UPM Institutional Repository
url_provider	http://psasir.upm.edu.my/
language	English
description	Vanadium-dependent haloperoxidases belong to a class of vanadium enzymes that may have potential industrial and pharmaceutical applications due to their high stability. In this study, the 5′-flanking genomic sequence and complete reading frame encoding vanadium-dependent bromoperoxidase (GcVBPO1) was cloned from the red seaweed, Fracilaria changii, and the recombinant protein was biochemically characterized. The deduced amino acid sequence of GcVBPO1 is 1818 nucleotides in length, sharing 49% identity with the vanadium-dependent bromoperoxidases from Corralina officinalis and Cor. pilulifera, respectively. The amino acid residues associated with the binding site of vanadate cofactor were found to be conserved. The Km value of recombinant GcVBPO1 for Br− was 4.69 mM, while its Vmax was 10.61 μkat mg−1 at pH 7. Substitution of Arg379 with His379 in the recombinant protein caused a lower affinity for Br−, while substitution of Arg379 with Phe379 not only increased its affinity for Br− but also enabled the mutant enzyme to oxidize Cl−. The mutant Arg379Phe was also found to have a lower affinity for I−, as compared to the wild-type GcVBPO1 and mutant Arg379His. In addition, the Arg379Phe mutant has a slightly higher affinity for H2O2 compared to the wild-type GcVBPO1. Multiple cis-acting regulatory elements associated with light response, hormone signaling, and meristem expression were detected at the 5′-flanking genomic sequence of GcVBPO1. The transcript abundance of GcVBPO1 was relatively higher in seaweed samples treated with 50 parts per thousand (ppt) artificial seawater (ASW) compared to those treated in 10 and 30 ppt ASW, in support of its role in the abiotic stress response of seaweed.
format	Article
author	Baharum, Hariyanti Chu, W. C. Teo, Swee Sen Ng, Kim Yong Abdul Rahim, Raha Ho, Chai Ling
spellingShingle	Baharum, Hariyanti Chu, W. C. Teo, Swee Sen Ng, Kim Yong Abdul Rahim, Raha Ho, Chai Ling Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta)
author_facet	Baharum, Hariyanti Chu, W. C. Teo, Swee Sen Ng, Kim Yong Abdul Rahim, Raha Ho, Chai Ling
author_sort	Baharum, Hariyanti
title	Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta)
title_short	Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta)
title_full	Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta)
title_fullStr	Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta)
title_full_unstemmed	Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta)
title_sort	molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (gcvbpo1) from gracilaria changii (rhodophyta)
publisher	Elsevier
publishDate	2013
url	http://psasir.upm.edu.my/id/eprint/28037/1/28037.pdf http://psasir.upm.edu.my/id/eprint/28037/ http://www.sciencedirect.com/science/article/pii/S0031942213001556
_version_	1643829350841188352
score	13.223943

Molecular cloning, homology modeling and site-directed mutagenesis of vanadium-dependent bromoperoxidase (GcVBPO1) from Gracilaria changii (Rhodophyta)

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