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CYP1A1 MspI polymorphism and cervical carcinoma risk in the multi-ethnic population of Malaysia: a case-control study

Background: Tobacco smoking is considered a risk factor for cervical cancer development due to the presence of tobacco based carcinogenic metabolites in cervical cells of female smokers. In this study, we investigated the role of the T3801C (MspI) polymorphism of CYP1A1, a gene encoding an enzyme ne...

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Main Authors: Tan, Yee Hock, Mohd Sidik, Shiran, Syed Hussain, Sharifah Noor Akmal, Lye, Munn Sann, Chong, Pei Pei
Format: Article
Language:English
Published: Asian Pacific Organization for Cancer Prevention 2016
Online Access:http://psasir.upm.edu.my/id/eprint/27982/1/CYP1A1%20MspI%20polymorphism%20and%20cervical%20carcinoma%20risk%20in%20the%20multi-ethnic%20population%20of%20Malaysia%20a%20case-control%20study.pdf
http://psasir.upm.edu.my/id/eprint/27982/
http://www.apocpcontrol.org/page/apjcp_issues_view.php?sid=Entrez:PubMed&id=pmid:26838255&key=2016.17.1.57
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spelling my.upm.eprints.279822016-06-20T05:54:19Z http://psasir.upm.edu.my/id/eprint/27982/ CYP1A1 MspI polymorphism and cervical carcinoma risk in the multi-ethnic population of Malaysia: a case-control study Tan, Yee Hock Mohd Sidik, Shiran Syed Hussain, Sharifah Noor Akmal Lye, Munn Sann Chong, Pei Pei Background: Tobacco smoking is considered a risk factor for cervical cancer development due to the presence of tobacco based carcinogenic metabolites in cervical cells of female smokers. In this study, we investigated the role of the T3801C (MspI) polymorphism of CYP1A1, a gene encoding an enzyme necessary for the initiation of tobacco based carcinogen metabolism, on cervical cancer risk. The T to C substitution may alter CYP1A1 activities, potentially elevating cervical cancer risk. Since results of gene-disease association studies vary according to the study population, the multi-ethnic population of Malaysia provides an excellent representative cohort for identifying and comparing the cervical cancer risk among the 3 major ethnics in Southeast Asia in relation to CYP1A1 MspI polymorphism. Materials and Methods: A total of 195 Thin Prep Pap smear samples from HPV negative and cancer free females were randomly selected as controls while 106 formalin fixed paraffin embedded samples from females with invasive cervical cancer were randomly selected for the cases group. The polymorphisms were identified using restriction fragment length polymorphism (RFLP) PCR. Results: We found no significant associations between CYP1A1 MspI polymorphism and cervical cancer in the general Malaysian female population. However, upon ethnic stratification, the variant C/C genotype was significantly associated with a 4.66-fold increase in cervical cancer risk in Malay females (95% CI= 1.21-17.9; p=0.03). No significant association was observed in the Chinese and Indian females. Additionally, there were no significant associations in the dominant model and allele frequency model analysis in both the general and ethnically stratified female population of Malaysia. Conclusions: Our findings suggest that the C/C genotype of CYP1A1 MspI polymorphism is associated with the development of cervical carcinoma in the Malay females of Malaysia. Asian Pacific Organization for Cancer Prevention 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/27982/1/CYP1A1%20MspI%20polymorphism%20and%20cervical%20carcinoma%20risk%20in%20the%20multi-ethnic%20population%20of%20Malaysia%20a%20case-control%20study.pdf Tan, Yee Hock and Mohd Sidik, Shiran and Syed Hussain, Sharifah Noor Akmal and Lye, Munn Sann and Chong, Pei Pei (2016) CYP1A1 MspI polymorphism and cervical carcinoma risk in the multi-ethnic population of Malaysia: a case-control study. Asian Pacific Journal of Cancer Prevention, 17 (1). pp. 57-64. ISSN 1513-7368 http://www.apocpcontrol.org/page/apjcp_issues_view.php?sid=Entrez:PubMed&id=pmid:26838255&key=2016.17.1.57 10.7314/APJCP.2016.17.1.57
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Background: Tobacco smoking is considered a risk factor for cervical cancer development due to the presence of tobacco based carcinogenic metabolites in cervical cells of female smokers. In this study, we investigated the role of the T3801C (MspI) polymorphism of CYP1A1, a gene encoding an enzyme necessary for the initiation of tobacco based carcinogen metabolism, on cervical cancer risk. The T to C substitution may alter CYP1A1 activities, potentially elevating cervical cancer risk. Since results of gene-disease association studies vary according to the study population, the multi-ethnic population of Malaysia provides an excellent representative cohort for identifying and comparing the cervical cancer risk among the 3 major ethnics in Southeast Asia in relation to CYP1A1 MspI polymorphism. Materials and Methods: A total of 195 Thin Prep Pap smear samples from HPV negative and cancer free females were randomly selected as controls while 106 formalin fixed paraffin embedded samples from females with invasive cervical cancer were randomly selected for the cases group. The polymorphisms were identified using restriction fragment length polymorphism (RFLP) PCR. Results: We found no significant associations between CYP1A1 MspI polymorphism and cervical cancer in the general Malaysian female population. However, upon ethnic stratification, the variant C/C genotype was significantly associated with a 4.66-fold increase in cervical cancer risk in Malay females (95% CI= 1.21-17.9; p=0.03). No significant association was observed in the Chinese and Indian females. Additionally, there were no significant associations in the dominant model and allele frequency model analysis in both the general and ethnically stratified female population of Malaysia. Conclusions: Our findings suggest that the C/C genotype of CYP1A1 MspI polymorphism is associated with the development of cervical carcinoma in the Malay females of Malaysia.
format Article
author Tan, Yee Hock
Mohd Sidik, Shiran
Syed Hussain, Sharifah Noor Akmal
Lye, Munn Sann
Chong, Pei Pei
spellingShingle Tan, Yee Hock
Mohd Sidik, Shiran
Syed Hussain, Sharifah Noor Akmal
Lye, Munn Sann
Chong, Pei Pei
CYP1A1 MspI polymorphism and cervical carcinoma risk in the multi-ethnic population of Malaysia: a case-control study
author_facet Tan, Yee Hock
Mohd Sidik, Shiran
Syed Hussain, Sharifah Noor Akmal
Lye, Munn Sann
Chong, Pei Pei
author_sort Tan, Yee Hock
title CYP1A1 MspI polymorphism and cervical carcinoma risk in the multi-ethnic population of Malaysia: a case-control study
title_short CYP1A1 MspI polymorphism and cervical carcinoma risk in the multi-ethnic population of Malaysia: a case-control study
title_full CYP1A1 MspI polymorphism and cervical carcinoma risk in the multi-ethnic population of Malaysia: a case-control study
title_fullStr CYP1A1 MspI polymorphism and cervical carcinoma risk in the multi-ethnic population of Malaysia: a case-control study
title_full_unstemmed CYP1A1 MspI polymorphism and cervical carcinoma risk in the multi-ethnic population of Malaysia: a case-control study
title_sort cyp1a1 mspi polymorphism and cervical carcinoma risk in the multi-ethnic population of malaysia: a case-control study
publisher Asian Pacific Organization for Cancer Prevention
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/27982/1/CYP1A1%20MspI%20polymorphism%20and%20cervical%20carcinoma%20risk%20in%20the%20multi-ethnic%20population%20of%20Malaysia%20a%20case-control%20study.pdf
http://psasir.upm.edu.my/id/eprint/27982/
http://www.apocpcontrol.org/page/apjcp_issues_view.php?sid=Entrez:PubMed&id=pmid:26838255&key=2016.17.1.57
_version_ 1643829335107305472
score 13.211869

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