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Probable impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells

Decline in the therapeutic potential of bone marrow-derived mesenchymal stem cells (MSC) is often seen with older donors as compared to young. Although hypoxia is known as an approach to improve the therapeutic potential of MSC in term of cell proliferation and differentiation capacity, its effects...

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Main Authors: Mohd Ali, Norlaily, Boo, Lily, Yeap, Swee Keong, Ky, Huynh, Satharasinghe, Dilan Amila, Liew, Woan Charn, Ong​, Han Kiat, Cheong, Soon Keng, Tunku Zainol Abidin, Tunku Kamarul Zaman
Format: Article
Language:English
Published: PeerJ 2016
Online Access:http://psasir.upm.edu.my/id/eprint/27951/1/27951.pdf
http://psasir.upm.edu.my/id/eprint/27951/
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spelling my.upm.eprints.279512017-04-21T08:25:53Z http://psasir.upm.edu.my/id/eprint/27951/ Probable impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells Mohd Ali, Norlaily Boo, Lily Yeap, Swee Keong Ky, Huynh Satharasinghe, Dilan Amila Liew, Woan Charn Ong​, Han Kiat Cheong, Soon Keng Tunku Zainol Abidin, Tunku Kamarul Zaman Decline in the therapeutic potential of bone marrow-derived mesenchymal stem cells (MSC) is often seen with older donors as compared to young. Although hypoxia is known as an approach to improve the therapeutic potential of MSC in term of cell proliferation and differentiation capacity, its effects on MSC from aged donors have not been well studied. To evaluate the influence of hypoxia on different age groups, MSC from young (< 30 years) and aged (> 60 years) donors were expanded under hypoxic (5% O2) and normal (20% O2) culture conditions. MSC from old donors exhibited a reduction in proliferation rate and differentiation potential together with the accumulation of senescence features compared to that of young donors. However, MSC cultured under hypoxic condition showed enhanced self-renewing and proliferation capacity in both age groups as compared to normal condition. Bioinformatic analysis of the gene ontology (GO) and KEGG pathway under hypoxic culture condition identified hypoxia-inducible miRNAs that were found to target transcriptional activity leading to enhanced cell proliferation, migration as well as decrease in growth arrest and apoptosis through the activation of multiple signaling pathways. Overall, differentially expressed miRNA provided additional information to describe the biological changes of young and aged MSCs expansion under hypoxic culture condition at the molecular level. Based on our findings, the therapeutic potential hierarchy of MSC according to donor's age group and culture conditions can be categorized in the following order: Young (hypoxia) > young (normoxia) > old aged (hypoxia) > old aged (normoxia). PeerJ 2016 Article PeerReviewed application/pdf en http://psasir.upm.edu.my/id/eprint/27951/1/27951.pdf Mohd Ali, Norlaily and Boo, Lily and Yeap, Swee Keong and Ky, Huynh and Satharasinghe, Dilan Amila and Liew, Woan Charn and Ong​, Han Kiat and Cheong, Soon Keng and Tunku Zainol Abidin, Tunku Kamarul Zaman (2016) Probable impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells. PeerJ, 4. art. no. e1536. pp. 1-19. ISSN 2167-8359 10.7717/peerj.1536
institution Universiti Putra Malaysia
building UPM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Putra Malaysia
content_source UPM Institutional Repository
url_provider http://psasir.upm.edu.my/
language English
description Decline in the therapeutic potential of bone marrow-derived mesenchymal stem cells (MSC) is often seen with older donors as compared to young. Although hypoxia is known as an approach to improve the therapeutic potential of MSC in term of cell proliferation and differentiation capacity, its effects on MSC from aged donors have not been well studied. To evaluate the influence of hypoxia on different age groups, MSC from young (< 30 years) and aged (> 60 years) donors were expanded under hypoxic (5% O2) and normal (20% O2) culture conditions. MSC from old donors exhibited a reduction in proliferation rate and differentiation potential together with the accumulation of senescence features compared to that of young donors. However, MSC cultured under hypoxic condition showed enhanced self-renewing and proliferation capacity in both age groups as compared to normal condition. Bioinformatic analysis of the gene ontology (GO) and KEGG pathway under hypoxic culture condition identified hypoxia-inducible miRNAs that were found to target transcriptional activity leading to enhanced cell proliferation, migration as well as decrease in growth arrest and apoptosis through the activation of multiple signaling pathways. Overall, differentially expressed miRNA provided additional information to describe the biological changes of young and aged MSCs expansion under hypoxic culture condition at the molecular level. Based on our findings, the therapeutic potential hierarchy of MSC according to donor's age group and culture conditions can be categorized in the following order: Young (hypoxia) > young (normoxia) > old aged (hypoxia) > old aged (normoxia).
format Article
author Mohd Ali, Norlaily
Boo, Lily
Yeap, Swee Keong
Ky, Huynh
Satharasinghe, Dilan Amila
Liew, Woan Charn
Ong​, Han Kiat
Cheong, Soon Keng
Tunku Zainol Abidin, Tunku Kamarul Zaman
spellingShingle Mohd Ali, Norlaily
Boo, Lily
Yeap, Swee Keong
Ky, Huynh
Satharasinghe, Dilan Amila
Liew, Woan Charn
Ong​, Han Kiat
Cheong, Soon Keng
Tunku Zainol Abidin, Tunku Kamarul Zaman
Probable impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells
author_facet Mohd Ali, Norlaily
Boo, Lily
Yeap, Swee Keong
Ky, Huynh
Satharasinghe, Dilan Amila
Liew, Woan Charn
Ong​, Han Kiat
Cheong, Soon Keng
Tunku Zainol Abidin, Tunku Kamarul Zaman
author_sort Mohd Ali, Norlaily
title Probable impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells
title_short Probable impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells
title_full Probable impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells
title_fullStr Probable impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells
title_full_unstemmed Probable impact of age and hypoxia on proliferation and microRNA expression profile of bone marrow-derived human mesenchymal stem cells
title_sort probable impact of age and hypoxia on proliferation and microrna expression profile of bone marrow-derived human mesenchymal stem cells
publisher PeerJ
publishDate 2016
url http://psasir.upm.edu.my/id/eprint/27951/1/27951.pdf
http://psasir.upm.edu.my/id/eprint/27951/
_version_ 1643829325876690944
score 13.211869

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